Method of controlling herbicide resistant weeds in crops

ABSTRACT

The present invention can provide a method having a high control effect on the removal of herbicide resistant weeds in a cultivation area for crops. The method includes a step of treating weeds with one or more of herbicides selected from the group consisting of saflufenacil, trifludimoxazin, flumioxazin, and ethyl [3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2, 4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetate.

TECHNICAL FIELD

This application claims priority to and the benefit of Japanese PatentApplication No. 2018-028514 filed on Feb. 21, 2018, the entire contentsof which are incorporated herein by reference.

The present invention relates to a method of controlling herbicideresistant weeds.

BACKGROUND ART

Saflufenacil, trifludimoxazin, flumioxazin, and ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetateare known as active ingredients for PPO inhibitors. It has been alsodisclosed that a certain type of PPO inhibitor controls PPO-inhibitorresistant Amaranthus palmeri having Gly210 deletion in PPO andPPO-inhibitor resistant ragweed having an Arg98Leu mutation.

CITATION LIST Patent Document

Patent Document 1: US6537948

Patent Document 2: US8987167

Patent Document 3: US8754008

Patent Document 4: WO2018/019842

Patent Document 5: WO2018/019845

Patent Document 6: WO2017/202774

SUMMARY OF THE INVENTION Problems to Be Solved by the Invention

It is an object of the present invention to provide a method having ahigh control effect on the control of herbicide resistant weeds in acultivation area for crops.

Means for solving the Problems

The present invention relates to a method of controlling specificPPO-inhibitor resistant weeds by treating the weeds with one or more ofherbicides selected from the group consisting of saflufenacil,trifludimoxazin, flumioxazin, and ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetranydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetate.

The present invention includes the following aspects.

[1] A method of controlling herbicide resistant weeds, the methodincluding a step of treating herbicide resistant weeds with one or moreof herbicides selected from the group consisting of saflufenacil,trifludimoxazin, flumioxazin, and ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetate,wherein the herbicide resistant weeds are PPO-inhibitor resistant weedshaving one or more mutations selected from the group consisting of anArg128Met mutation, Arg128Gly mutation, Arg128His mutation, andGly399Ala mutation in PPO.

[2] The control method according to [1], wherein the herbicide resistantweed is Amaranthus palmeri, waterhemp, or ragweed.

[3] The control method according to [1] or [2], wherein the method beingperformed in cultivation of a crop.

[4] The control method according to [3], wherein the crop is oneselected from the group consisting of a soybean, corn, cotton, rapeseed, rice, wheat, barley, sugarcane, sugar beet, sorghum, andsunflower.

[5] The control method according to [3] or [4], wherein the crop is acrop to which tolerance to saflufenacil, trifludimoxazin, flumioxazin,or ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetateis imparted.

[6] The control method according to any one of [1] to [5], wherein theherbicide resistant weed is a lactofen resistant weed or fomesafenresistant weed.

Effect of the Invention

Herbicide resistant weeds in a cultivation area for crops can becontrolled by the method of controlling herbicide resistant weedsaccording to the present invention.

Mode for Carrying out the Invention

In the method of controlling herbicide resistant weeds according to thepresent invention, saflufenacil, trifludimoxazin, flumioxazin, or ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetateis applied in a cultivation area for crops before, when, and/or aftersowing crop seeds or in a non-cultivated field.

Examples of the crop in the present invention include corn, cotton, rapeseed, rice, wheat, barley, sugarcane, sugar beet, sorghum, andsunflower. However, no limitation is imposed on the crop as long as itis a type which is usually cultivated as a crop.

The aforementioned plants may be plants producible by natural crossing,plants producible by a mutation, F1 hybrid plants, or transgenic plants(also called genetically modified plants). These plants generally havecharacteristics such as impartment of tolerance to herbicides,accumulation of substances harmful to pests, reduction in sensitivity todiseases, increase in yield potential, improvement in resistance tobiological or non-biological stress factors, accumulation of substances,and improvement in preservability and processability.

The F1 hybrid plants are those which are each a first filial hybridobtained by crossing two different varieties with each other and areusually those having characteristics superior in heterosis, that is anature more excellent than both of the parents. The transgenic plantsare those which are obtained by introducing an exogeneous gene fromother organisms such as microorganisms and have characteristics likethose that cannot be easily obtained by crossbreeding, mutationinduction, or natural recombination in natural environments.

Examples of the technologies used to create the above plants includeconventional type variety improvement; genetic recombinationtechnologies; genome breeding technologies; new breeding technologies;and genome editing technologies. The conventional type varietyimprovement is specifically a technology for obtaining plants havingdesired properties by a spontaneous mutation and crossing. The geneticrecombination technologies are technologies in which a target gene (DNA)is extracted from a certain organism (for example, microorganism) tointroduce it into a genome of a different target organism, thereby newproperties to the organism, and antisense technologies or RNAinterference technologies for imparting new or improved characteristicsby silencing other genes existing in plants. The genome breedingtechnologies are those improving breeding efficiency by using genomeinformation and include DNA marker (also called genome markers orgenetical markers) breeding technologies and genomic selection. Forexample, the DNA marker breeding is a method in which a progeny having agene with a target and useful trait is selected from a lot of crossprogenies by using a DNA marker which is a DNA sequence and is a targetof the presence position of a gene with a specific useful trait on agenome. This method has the characteristics that the time required forbreeding can be efficiently reduced by analyzing the cross progeny byusing a DNA marker when the progeny is a juvenile plant.

Also, the genomic selection is a technique in which a prediction formulais created from a phenotype obtained in advance and genome informationto predict the characteristics from the prediction formula and thegenome information without any evaluation of the phenotype and istechnologies contributing to improvement in efficient breeding. The newbreeding techniques are a generic term of variety-improvement=breeding)techniques that are combinations of molecular biological techniques.Examples of the new breeding techniques include cisgenesis/intragenesis,introduction of an oligonucleotide-directed mutation, RNA-dependent DNAmethylation, genome editing, grafting onto a GM rootstock or scion,reverse breeding, agroinfiltration, and seed production technology(SPT). The genome editing technologies are those in which geneinformation is transformed in a sequence-specific manner which enables,for example, deletion of a base sequence, substitution of an aminosequence, and introduction of an exogenous gene.

Examples of tools for these techniques include sequence-specific genomemodification techniques such as zinc-finger nuclease (ZFN), TALEN,CRISPR/Cas9, CRISPER/Cpfl, and Meganuclease which each enablesequence-specific DNA scission and CAS9 Nickase and Target-AID which areeach created by modifying the aforementioned tools.

Examples of the plants mentioned above include plants listed in GMAPPROVAL DATABASE of genetically modified crops in the electronicinformation site (http://www.isaaa.org/) of INTERNATIONAL SERVICE forthe ACQUISITION of AGRI-BIOTECH APPLICATIONS (ISAAA). More specifically,these examples include herbicide tolerant plants, harmful insecttolerant plants, disease tolerant plants, and quality modified (forexample, increase or decrease in content or change in composition)plants of products (for example, starch, amino acid, and fatty acid),fertile trait modified plants, abiotic stress tolerant plants, or plantsmodified in traits relating to growth and yield.

Examples of plants imparted with tolerance to herbicides are given asfollows.

The mechanism of tolerance to herbicides is obtained, for example, byreducing the compatibility of a chemical with its target, by rapidmetabolism (for example, breakdown or modification) resulting from theexpression of a chemical deactivation enzyme, or by inhibiting theincorporation of a chemical into a plant body or the transfer of thechemical in the plant body.

The plants to which herbicide tolerance is imparted by geneticrecombination technologies include plants to which tolerances to thefollowing inhibitors are imparted by genetic recombination technologies:4-hydroxyphenyl pyruvate dioxygenase (hereinafter abbreviated as HPPD)inhibitors such as isoxaflutole and mesotrione, acetolactate synthetase(hereinafter:abbreviated as ALS) inhibitors such as imidazolinone typeherbicides containing imazethapyr and sulfonylurea type herbicidescontaining thifensulfurcn-methyl, 5-enolpyruvylshikimate-3-phosphatesynthase (hereinafter abbreviated as EPSP) inhibitors such asglyphosate, glutamine synthetase inhibitors such as glufosinate, auxintype herbicides such as 2,4-D and dicamba, and oxynil type herbicidescontaining bromoxynil. Preferable examples of the transgenic plantstolerant to herbicides include cereals such as wheat, barley, rye, andoats, and canola, sorghum, soybean, rice, rape seed, sugar beet,sugarcane, grape, lentil, sunflower, alfalfa, pome fruits, stone fruits,coffee, tea, strawberry, turf grass, and vegetables such as tomato,potato, cucumber, and lettuce, and among them, cereals such as wheat,barley, rye, and oats and soybean, rice, vine, tomato, potato, and pomefruits are more preferable. Examples of the herbicide tolerant plantsare shown below:

Glyphosate herbicide tolerant plants: which are obtained by introducingone or more of a glyphosate tolerant EPSPS gene (CP4 epsps) derived fromAgrobacterium tumefaciens strain CP4, glyphosate metabolism enzyme gene(gat4601, gat4621) obtained by strengthening the metabolism activity ofa glyphosate metabolism enzyme (glyphosate N-acetyltransferase) genederived from Bacillus licheniformis by shuffling technologies,glyphosate metabolism enzyme (glyphosate oxidase) gene (goxv247) derivedfrom Ochrobacterum anthropi strain (LBAA), or EPSPS gene (mepsps,2mepsps) having a glyphosate tolerance mutation derived from corn.Examples of main plants include alfalfa (Medicago sativa), Argentinecanola (Brassica napus), cotton (Gossypium hirsutum L.), creepingbentgrass (Agrostis stolonifera), corn (Zea mays L.), polish canola(Brassica rapa), potato (Solanum tubeHDDrosum L.), soybean (Glycine maxL.), sugar beet (Beta vulgaris), and wheat (Triticum aestivum). Some ofthese glyphosate resistant transgenic plants are commercially available.For example, genetically modified plants expressing glyphosate tolerantEPSPS genes derived from Agrobacterium strains are commerciallyavailable under the names including “Roundup Ready (registeredtrademark)”, genetically modified plants expressing a glyphosatemetabolism enzyme which is derived from Bacillus subtilis and obtainedby strengthening its metabolism activity by shuffling technologies arecommercially available under the names of “Optimum (registeredtrademark) GAT (trademark)”, “Optimum (registered trademark) Glycanola”, and the like. Genetically modified plants expressing the EPSPSgenes having a glyphosate tolerance mutation derived from corn arecommercially available under the name of “GlyTol (trademark)”.

Glufosinate herbicide tolerant plants: which are obtained by introducingone or more of a phosphinothricin N-acetyltransferase (PAT) gene (bar)which is a glufosinate metabolism enzyme derived from Streptomyceshygroscopicus, phosphinothricin N-acetyltransferase (PAT) enzyme gene(pat) which is a glufosinate metabolism enzyme derived from Streptomyesviridochromogenes, and a synthesized pat gene (pat syn) derived fromStreptomyes viridochromogenes strain Tu494. Examples of main plantsinclude Argentine canola (Brassica napus), chicory (Cichorium intybus),cotton (Gossypium hirsutum L.), corn (Zea mays L.), polish canola(Brassica rapa), rice (Oryza sativa L.), soybean (Glycine max L.), andsugar beet (Beta vulgaris). Some of these glufosinate toleranttransgenic plants are commercially available. For example, geneticallymodified plants derived from a glufosinate metabolism enzyme (bar)derived from Streptomyces hygroscopicus and genetically modified plantsderived from Streptomyes viridochromogenes are commercially availableunder the names including “LibertyLink (trademark)”, “InVigor(trademark)”, and “WideStrike (trademark)”. Oxynil type herbicides (forexample, “bromoxynil” tolerant plants: there are oxynil type herbicides,for example, bromoxynil tolerant transgenic plants, which are obtainedby introducing a nitrilase gene (bxn) which is an oxynil type herbicide(for example, bromoxynil) metabolism enzyme derived from Klebsiellapneumoniae subsp.Ozaenae). Examples of main plants include Argentinecanola (Brassica napus), cotton (Gossypium hirsutum L.), and tobacco(Nicotiana tabacum L.). These plants are commercially available underthe names including “Navigator (trademark) canola” and “BXN(trademark)”. There are also the following plants commercially availableunder the following names: ALS herbicide tolerant plants: carnations(Dianthus caryophyllus) “Moondust (trademark)”, “Moonshadow(trademark)”, “Moonshade (trademark)”, “Moonlite (trademark)”, “Moonaqua(trademark)”, “Moonvista (trademark)”, “Moonique (trademark)”,“Moonpearl (trademark)”, “Moonberry (trademark)”, and “Moonvelvet(trademark)” obtained by introducing an ALS herbicide tolerant ALS gene(surB) derived from tobacco (Nicotiana tabacum) as a selective marker;Flax (Linum usitatissumum L.) “CDC Triffid Flax” obtained by introducingan ALS herbicide tolerant ALS gene (als) derived from thale cress(Arabidopsis thaliana); Corn (Zea mays L.) “Optimum (trademark) GAT(trademark)” which is obtained by introducing an ALS herbicide tolerantALS gene (zm-hra) derived from corn and is tolerant to sulfonylurea-typeand imidazolinone-type herbicides; Soybean “Cultivance” which isobtained by introducing an ALS herbicide tolerant ALS gene (csr1-2)derived from thale cress and is tolerant to imidazolinone typeherbicides; and Soybean “Treus (trademark)”, “Plenish (trademark)”, and“Optimum GAT (trademark)”, which is obtained by introducing an ALSherbicide tolerant ALS gene (gm-hra) derived from soybean (Glycine max)and is tolerant to sulfonylurea type herbicides. Also, cotton isavailable into which an ALS herbicide tolerant ALS gene derived fromtobacco (Nicotiana tabacum cv. Xanthi) is introduced. HPPD herbicidetolerant plants: soybean into which mesotrione tolerant HPPD genes(avhppd-03) derived from oats (Avena sativa) and phosphinothricinN-acetyltransferase (PAT) enzyme genes (pat) tolerant to phomesotrionewhich is a glufosinate metabolism enzyme derived from Streptomyesviridochromogenes are both introduced is commercially available underthe name of “Herbicide-tolerant Soybean line (trademark)”.

2,4-D tolerant plants: corn obtained by introducing aryloxyalkanoatedioxygenase genes (aad-1) which are a 2,4-D metabolism enzyme derivedfrom Sphingobium herbicidovorans is commercially available under thename of “Enlist (trademark) maize”. There are soybean and cottonobtained by introducing an allyloxyalkanoate dioxygenase gene (aad-12)which is a 2,4-D metabolism enzyme derived from Delftia acidovorans andthese plants are commercially available under the name of “Enlist(trademark) Soybean”.

Dicamba tolerant plants: there are soybean and cotton obtained byintroducing a Dicamba monooxygenase gene (dmo) which is a dicambametabolism enzyme derived from Stenotrophomonas maltophilia strain DI-6.Soybean (Glycine max L.) obtained by introducing, in addition to theabove gene, a glyphosate tolerant type EPSPS gene (CP4 epsps) derivedfrom Agrobacterium tumefaciens strain CP4 is commercially availableunder the name of “Genuity (registered trademark) Roundup Ready(trademark) 2 Xtend (trademark)”.

Examples of commercially available products of the transgenic plants towhich herbicide tolerance is imparted include glyphosate tolerant corn:“Roundup Ready Corn”, “Roundup Ready 2”, “Agrisure GT”, “AgrisureGT/CB/LL”, “Agrisure GT/RW”, “Agrisure 3000GT”, YieldGard VTRootworm/RR2″, and “YieldGard VT Triple”; glyphosate resistant soybean:“Roundup Ready Soybean” and “Optimum GAT”; glyphosate tolerant cotton:“Roundup Ready Cotton” and “Roundup Ready Flex”; glyphosate tolerantcanola: “Roundup Ready Canola”; glyphosate tolerant alfalfa “RoundupReady Alfalfa”; glyphosate tolerant rice: “Roundup Ready Rice”;glufosinate tolerant corn: “Roundup Ready 2”, “Liberty Link”, “Herculex1”, “Herculex RW”, “Herculex Xtra”, “Agrisure GT/CB/LL”, “AgrisureCB/LL/RW”, and “Bt10”; glufosinate tolerant cotton: “FiberMax LibertyLink”; glufosinate tolerant rice: “Liberty Link Rice”; glufosinatetolerant canola: “InVigor”; glufosinate tolerant rice: “Liberty LinkRice” (product from Bayer); bromoxynil tolerant cotton: “BXN”;bromoxynil tolerant canola: “Navigator” and “Compass”; and glufosinatetolerant canola; “InVigor”. Further plants modified in respect toherbicides are widely known. Examples of these plants include glyphosatetolerant plants such as alfalfa, apple, barley, eucalyptus, flax, grape,lentil, rape seed, pea, potato, rice, sugar beet, sunflower, tobacco,tomato, turf grass, and wheat (see, for example, U.S. Pat. Nos.5,188,642, 4,940,835, 5,633,435, 5,804,425, and 5,627,061); dicambatolerant plants such as bean, cotton, soybean, pea, potato, sunflower,tomato, tobacco, corn, sorghum, and sugarcane (see, for example,WO2008051633, U.S. Pat. Nos. 7,105,724, and 5,670,454); glufosinatetolerant plants such as soybean, sugar beet, potato, tomato, and tobacco(see, for example, U.S. Pat. Nos. 6,376,754, 5,646,024, and 5,561,236);2,4-D resistant plants such as cotton, peppers, apple, tomato,sunflower, tobacco, potato, corn, cucumber, wheat, soybean, sorghum, andcereal crops (see, for example, U.S. Pat. No. 6,153,401, 6,100,446,WO2005107437, U.S. Pat. No. 5,608,147, and U.S. Pat. No. 5,670,454);plants resistant to acetolactate synthase (ALS) inhibitors (for example,sulfonylurea type herbicides and imidazolinone type herbicides) such ascanola, corn, millet, barley, cotton, Indian mustard, lettuce, lentil,melon, millet, oat, rape seed, potato, rice, rye, sorghum, soybean,sugar beet, sunflower, tobacco, tomato, and wheat (see, for example,U.S. Pat. No. 5,013,659, WO2006060634, U.S. Pat. Nos. 4,761,373,5,304,732, 6,211,438, 6,211,439, and 6,222,100), particularly, ricetolerant to imidazolinone type herbicides is well known, and rice andthe like having specific mutations (for example, S653N, S654K, A122T,S653(At)N, S654(At)K, A122(At)T) in an acetohydroxyacid synthase geneare well known (see, for example, US 2003/0217381 and WO200520673); andplants resistant to HPPD inhibitors (for example, isoxazole typeherbicides such as isoxaflutole, triketone type herbicides such assulcotrione and mesotrione, pyrazole type herbicides such aspyrazolynate, and diketonitrile which is a degradation product ofisoxaflutole), such as barley, sugarcane, rice, corn, tobacco, soybean,cotton, rape seed, sugar beet, wheat, and potato (see, for example,WO2004/055191, WO199638567, WO1997049816 and U.S. Pat. No. 6,791,014).

Examples of plants to which herbicide tolerance is imparted classicallyor by genome breeding technologies include rice “Clearfield Rice”, wheat“Clearfield wheat”, sunflower “Clearfield Sunflower”, “lentil(Clearfield lentils”, and canola “Clearfield canola” (BASF products),which are each tolerant to imidazolinone type ALS inhibitors such asimazethapyr and imazamox; soybean “STS soybean” tolerant to sulfonyltype ALS inhibitors such as thifensulfuron methyl; sethoxydim tolerantcorn “SR corn” and “Poast Protected (registered trademark) corn” havingtolerance to acetyl CoA carboxylase inhibitors such as trione-oxime typeor aryloxyphenoxypropionate type herbicides; sunflower “ExpressSun(registered trademark)” having tolerance to sulfonylurea type herbicidessuch as tribenuron; rice “Provisia (trademark) Rice” having tolerance toacetyl CoA carboxylase inhibitors such as quizalofop; and canola“Triazinon Tolerant Canola” having tolerance to a PSII inhibitor.

Examples of plants to which herbicide tolerance is imparted by genomeediting technologies include canola “Su Canola (registered trademark)”having tolerance to sulfonylurea type herbicides and which are developedusing Rapid Trait Development System (RIDS) (registered trademark). ThisRTDS (registered trademark) is a technology corresponding to theintroduction of an oligonucleotide-directed mutation in genome editingtechnologies and is a technology enabling the introduction of a mutationthrough Gene Repair Oligonucleotide (GRON), that is, DNA-RNA chimericoligonucleotide without cutting of DNA in the plant. Also, herbicidetolerant corn reduced in phytic acid content by using zinc fingernuclease to delete an endogenous gene IPK1 (see, for example, Nature459, 437-441, 2009); and an example in which herbicide tolerance isimparted to rice by using CRISPR/Cas9 are given (see, for example, Rice,7, 5 2014).

In the present invention, examples of the crops tolerant to specific PPOinhibitors include those provided with PPO reduced in compatibility withthe inhibitors by genetic recombination technologies. Or, a substancewhich can detoxify and decompose these PPO inhibitors by the aid ofcytochrome P450 monooxygenase may be contained either independently orin combination with the above PPO. These tolerant crops are described inpatent documents such as WO2011085221, WO2012080975, WO2014030090,WO2015022640, WO2015022636, WO2015022639, WO2015092706, WO2016203377,WO2017198859, WO2018019860, WO2018022777, WO2017112589, WO2017087672,WO2017039969, WO2017023778, and non patent documents (Pest ManagementScience, 61, 2005, 277-285).

With regard to the plants to which herbicide tolerance is imparted,examples in which the nature of a rootstock is transferred to a scion inbreeding technologies utilizing grafting include an example in whichglyphosate tolerance is imparted to a scion of non-transgenetic soybeanby using Roundup Ready (registered trademark) soybean having glyphosatetolerance as the rootstock (see, Weed Technology 27:412-416, 2013).

Examples of plants to which pest resistance is imparted are shown below.

Examples of plants to which resistance to lepidopterous pests isimparted by genetic recombination technologies include plants such ascorn (Zea mays L.), soybean (Glycine max L.), cotton (Gossypium hirsutumL.), rice (Oryza sativa L.), poplar (Populus sp.), tomato (Lycopersiconesculentum), and eggplant (Solanum melongena), each obtained byintroducing a gene encoding δ-endotoxin which is an insecticidal proteinderived from Bacillus thuringiensis (hereinafter abbreviated as Btbacteria) which is soil bacteria. Examples of the δ-endotoxin impartingresistance to lepidopterous pests include Cry1A, Cry1Ab, modified Cry1Ab(partially deficient Cry1Ab), Cry1Ac, Cry1Ab-Ac(hybrid protein ofcombined Cry1Ab and Cry1Ac), Cry1C, Cry1F, Cry1Fa2 (modified cry1F),moCry1F (modified Cry1F), Cry1A.105 (hybrid protein of combined Cry1Ab,Cry1Ac, and Cry1F), Cry2Ab2, Cry2Ae, Cry9C, Vip3A, and Vip3Aa20.Examples of plants to which resistance to coleopterous pests is impartedby genetic recombination technologies include plants such as corn andpotato, each obtained by introducing a gene encoding δ-endotoxin whichis an insecticidal protein derived from Bt bacteria which are soilbacteria. Examples of the δ-endotoxin imparting resistance tocoleopterous pests include Cry3A, mCry3A (modified Cry3A), Cry3Bbl,Cry34Ab1, and Cry35Ab1. Examples of plants to which resistance todipterous pests is imparted by genetic recombination technologiesinclude plants such as genetically modified corn (Zea mays L.) obtainedby introducing a synthesized gene encoding a hybrid protein eCry3.1Ab ofa combination of Cry3A and Cry1Ab derived from Bt bacteria which aresoil bacteria, and genetically modified cotton (Gossypium hirsutum L.)obtained by introducing a gene encoding a trypsin inhibitor CpTI derivedfrom cowpea (Vigna unguiculate). Further examples include geneticallymodified poplar obtained by introducing a gene encoding API which is aprotease inhibitor protein A derived from arrowhead (Sagittariasagittifolia). These plants have tolerance to a wide range of pests.

The insecticidal proteins imparting pest resistance to plants includehybrid proteins of the above insecticidal proteins, partially deficientproteins, and modified proteins. The hybrid proteins are produced by acombination of different domains of a plurality of insecticidal proteinsand for example, Cry1Ab-Ac and Cry1A.105 are known. As the partiallydeficient proteins, Cry 1Ab deficient in a part of amino acid sequencesis known. As the modified proteins, Cry1Fa2, moCry1F, mCry3A, and thelike are known that are proteins in which one or plural amino acids ofnatural type δ-endotoxin are substituted. Also, in the amino acidsubstitution like this, a protease recognition sequence which does notexist in nature is preferably inserted into toxin as shown in the case(see WO2003/018810) of Cry3A055.

Monsanto Company has developed cotton (event MON88702) obtained byintroducing a modified BT protein Cry51Aa2 (Cry51Aa2.834_16) by geneticrecombination technologies and the cotton has resistance to genus lygussuch as Lygus lineolaris, Hemiptera such as aphid and Thysanoptera suchas genus Frankliniella.

Other than the above, examples of the insecticidal protein impartingpest resistance to plants by genetic recombination technologies includeinsecticidal proteins derived from Bacillus cereus or Bacilluspopilliae, vegetable proteins Vip1, Vip2, Vip3, and Vip3A derived fromBt bacterium strain AB88, insecticidal proteins derived from nematodesymbiotic (forms a colony in nematode) bacteria, for example,Photorhabdus spp. such as Photorhabdus luminescens and Xenorhabdusnematophilus and Xenorhabdus spp. such as Xenorhabdus nematophilus,toxins produced from animals having neurotoxins specific to insects,such as a scorpion toxin, spider toxin, and bee toxin, toxins offilamentous fungi such as a Streptomycetes toxin, vegetable lectin suchas pea lectin, barley lectin, and snowdrop lectin, protease inhibitorssuch as agglutinin, trypsin inhibitor, serine protease inhibitor,protease inhibitors such as patatin, cystatin, and papain inhibitor,ribosome inactivation proteins (RIP) such as lysine, corn-RIP, abrin,luffin, saporin, and bryodin, steroid metabolism enzymes such as3-hydroxysteroidoxydase, ecdysteroid-UDP-glucosyltransferase, andcholesterol oxidase, ion channel inhibitors such as an ecdysoneinhibitor, HMG-CoA-reductase, and sodium channel or calcium channelinhibitor, juvenile hormone esterase, diuretic hormone receptor,stilbene synthase, bibenzyl synthase, chitinase, and glucanase.

Plants to which pest resistance is imparted by introducing one or two ormore insecticidal protein genes have been already known and some ofthese plants are commercially available. Examples of cotton having pestresistance include “Bollgard (trademark) cotton”, “BXN (trademark) PlusBollgard (trademark) Cotton”, “BXN (trademark) Plus Bollgard (trademark)Cotton”, “JK 1”, “Roundup Ready (trademark) Bollgard (trademark)Cotton”, and “Ingard (trademark)”, which each express an insecticidalprotein Cry1Ac derived from Bt bacteria, “Herculex(trademark) I” and“Herculex(trademark) CB”, which each express an insecticidal proteinmodified Cry1F (Cry1Fa2) derived from Bt bacteria; “VIPCCT (trademark)Cotton” expressing an insecticidal protein Vip3A derived from Btbacteria; “Bollgard II (trademark) cotton”, “Roundup Ready(trademark)Bollgard II (trademark) Cotton”, “Roundup Ready (trademark) Flex(trademark) Bollgard II (trademark) Cotton” and “Fivermax (trademark)Liberty Link (trademark) Bollgard II (trademark)”, which each expressinsecticidal proteins Cry1Ac and Cry2Ab derived from Bt bacteria;“Bollgard III (registered trademark) cotton” and “Bollgard (registeredtrademark) III×Roundup Ready (trademark) Flex (trademark)”, whichexpress insecticidal proteins Cry1Ac, Cry2Ab, and Vip3A derived from Btbacteria, “VIPCOT (trademark) Roundup Ready Flex (trademark) Cotton”expressing insecticidal proteins Vip3A and Cry1Ab derived from Btbacteria; “VIPCOT (registered trademark)” expressing insecticidalproteins Vip3A and Cry1Ac derived from Bt bacteria; “WideStrike(trademark) Cotton”, “WideStrike (trademark) Roundup Ready (trademark)Cotton”, and “Widestrike (trademark) Roundup Ready Flex (trademark)Cotton”, which express insecticidal proteins Cry1Ac and Cry1F derivedfrom Bt bacteria; “VIPCOT (trademark)” Cotton expressing an insecticidalprotein Vip3A derived from Bt bacteria; “Twinlink (trademark) Cotton”and “Glytol (trademark)×Twinlink (trademark), which express insecticidalproteins Cry1Ab and Cry2Ae derived from Bt bacteria; “Widestrike(registered trademark) 3” and “Widestrike (trademark)×Roundup Ready Flex(trademark)×VIPCOT (trademark) Cotton”, which express insecticidalproteins Cry1Ac, Cry1F, and Vip3A derived from Bt bacteria; and “Glytol(trademark)×Twinlink (trademark)×VIPCOT (trademark) Cotton” expressinginsecticidal proteins Cry1Ab, Cry2Ae, and Vip3A derived from Btbacteria”.

Examples of the corn having pest resistance include “YieldGard(registered trademark) Rootworm RW”, “YieldGard (trademark) RW+RR”,“YieldGard (trademark) VT (trademark) Rootworm (trademark) RR2”, and“MaxGard (trademark)”, which express an insecticidal protein Cry3Bblderived from Bt bacteria; “YieldGard (registered trademark) VT Triple”and “YieldGard (trademark) Plus with RR”, which express insecticidalproteins Cry3Bb1 and Cry1Ab derived from Bt bacteria; “Bt Xtra(trademark) Maize” expressing an insecticidal protein Cry1Ac derivedfrom Bt bacteria; “YieldGard Plus (registered trademark)” expressinginsecticidal proteins Cry1Ab and Cry3Bb1 derived from Bt bacteria;“Bt10”, “Liberty Link(trademark) Yieldgard (trademark) Maize”, “Agrisure(trademark) GT/CB/LL”, and “YieldGard (trademark) CB+RR” expressing aninsecticidal protein Cry1Ab derived from Bt bacteria; “YieldGard(trademark) VT Pro (trademark)” and “tenuity (registered trademark) VTDouble Pro (trademark)”, which express insecticidal proteins Cry1A. 105and Cry2Ab2 derived from Bt bacteria; “Agrisure (registered trademark)RW” and “Agrisure (trademark) GT/RW”, which express an insecticidalprotein mCry3A derived from Bt bacteria; “Starlink (trademark) Maize”expressing an insecticidal protein Cry9C derived from Bt bacteria;“YieldGard (trademark)”, “MaizeGard (trademark)”, “NaturGard KnockCut(trademark)”, “Maximizer (trademark)”, “Roundup Ready (trademark)YieldGard (trademark) Maize”, “Agrisure (trademark) CB/LL”, and “Mavera(trademark) YieldGard (trademark) Maize”, which express an insecticidalprotein Cry1Ab derived from Bt bacteria; “Agrisure (registeredtrademark) 3122” expressing insecticidal proteins Cry1Ab, Cry1F,modified Cry3A, Cyr34Ab1, and Cyr35Ab1 derived from Bt bacteria;“Agrisure (registered trademark) Viptera” expressing an insecticidalprotein Vip3Aa20 derived from Bt bacteria; “Agrisure (registeredtrademark) Viptera (trademark) 2100” and “Agrisure (registeredtrademark) Viptera (trademark) 3110”, which express insecticidalproteins Vip3Aa20 and Cry1Ab derived from Bt bacterial; “Agrisure(registered trademark) Viptera (trademark) 3100”, “Agrisure(registeredtrademark) Viptera (trademark) 3111” and “Agrisure (registeredtrademark) Viptera (trademark) 4”, which express insecticidal proteinsVip3Aa20, Cry1Ab, and modified Cry3A derived from Bt bacteria; “Agrisure(registered trademark) Viptera (trademark) 3220” expressing insecticidalproteins Vip3Aa20, Cry1Ab, and modified Cry1F derived from Bt bacteria;“Agrisure (registered trademark” “Duracade (trademark)” expressing aninsecticidal protein eCry3.1Ab (Cry3A-Cry1Ab chimera protein) derivedfrom Bt bacteria; “Agrisure (registered trademark) Duracade (trademark)5122” expressing insecticidal proteins eCry3.1Ab (Cry3A-Cry1Ab chimeraprotein), modified Cry3A, Cry1Ab, and modified Cry1F derived from Btbacteria; “Agrisure (registered trademark) Duracade (trademark) 5222”expressing insecticidal proteins eCry3.1Ab (Cry3A-Cry1Ab chimeraprotein), modified Cry3A, modified Cry1Ab, and Vip3A variant derivedfrom Bt bacteria; “Herculex (trademark) RW” expressing insecticidalproteins Cyr34Ab1 and Cyr35Ab1 derived from Bt bacteria; “Herculex XTRA(trademark)” expressing insecticidal proteins Cyr34Ab1, Cyr35Ab1, andCry1F derived from Bt bacteria; “Genuity (registered trademark) VTTriple Pro (trademark)” expressing insecticidal proteins Cry1A. 105,Cry2Ab2, and Cry3Bb1 derived from Bt bacteria; “Genuity (registeredtrademark) SmartStax (trademark)” expressing insecticidal proteinsCry1F, Cry2Ab, Cyr34Ab1, Cyr35Ab1, Cry3Bb1, and Cry1A. 105 derived fromBt bacteria; “Power Core (trademark)” expressing insecticidal proteins,modified Cry1F, Cry2Ab, and Cry1A. 105 derived from Bt bacteria;“Herculex XTRA (trademark) RR” expressing insecticidal proteins Cry1F,Cyr34Ab1, and Cyr35Ab1 derived from Bt bacteria; “Optimum (registeredtrademark) Intrasect Xtreme” expressing insecticidal proteins, modifiedCry1F, Cyr34Ab1, Cyr35Abl, Cry1Ab, and modified Cry3A derived from Btbacteria; “Optimum (registered trademark) Intrasect XTRA” expressinginsecticidal proteins, modified Cry1F, Cyr34Ab1, Cyr35Ab1, and Cry1Abderived from Bt bacteria; and “Optimum (registered trademark) TRIsect”expressing insecticidal proteins, modified Cry1F and modified Cyr3Aderived from Bt bacteria: these products being all commerciallyavailable.

Examples of other plants having pest resistance include potato “AtlanticNewLeaf (trademark) potato”, “NewLeaf (trademark) Russet Burbankpotato”, “Lugovskoi plus”, “Elizaveta plus”, “Hi-Lite NewLeaf(trademark) Y potato”, “Superior NewLeaf (trademark) potato”, and“Shepody NewLeaf (trademark) Y potato”, which express an inseccidalprotein Cry3A derived from Bt bacteria; rice “hanyou 63” and “Huahui-1”,which express insecticidal proteins Cry1Ab and Cry1Ac derived from Btbacteria; soybean “Intacta (trademark) Roundup Ready (trademark) 2 Pro”expressing an insecticidal protein Cry1Ac derived from Bt bacteria; andeggplant “BARI Bt Begun-1, -2, -3, and -4” expressing an insecticidalprotein Cry1Ac derived from Bt bacteria, these products being eachcommercially available.

Specifically, the following plant products are available: corn“YieldGard corn rootworm” and “YieldGard VT”, “Herculex RW” and“Herculex Rootworm”, and “Agrisure CRW”, which have resistance to cornrootworms; corn “YieldGard corn borer”, “YieldGard plus” and “YieldGardVT Pro”, “Agrisure CB/LL” and “Agrisure 3000GT”, “Hercules I”, and“Hercules II”, “KnockOut”, “NatureGard”, and “StarLink”, which haveresistance to corn borers; corn “Herculex I” and “Herculex Xtra”,“NewLeaf”, “NewLeaf Y”, and “NewLeaf Plus”, which have resistance towestern bean cutworms, corn borers, black cutworms, and fall armyworms;corn “YieldGard Plus” having resistance to corn borers and cornrootworms; cotton “Bollgard I” and “Bollgard II”, which have resistanceto Heliothis virescens; cotton “Bollgard II”, “WideStrike”, and“VipCot”, which have resistance to Heliothis virescens, cottonbollworms, fall armyworms, beet armyworms, cabbage loopers, soybeanloopers, and pink bollworms; potato “NewLeaf”, “NewLeaf Y”, and “NewLeafPlus”, which have resistance to Manduca sexta; and eggplant “Btbrinjal”, “Dumaguete Long Purple”, and “Mara”, which have resistance toLeucimodes orbonalis, fruit borers, and cotton bollworms; (see, forexample, U.S. Pat. No. 5,128,130).

Further plants having insecticidal resistance are usually known andexamples of these plants include rice having resistance to yellow riceborers (see, for example, Molecular Breeding, vol 18 (2006), No. 1),lettuce having resistance to Lepidoptera (see, for example, U.S. Ser.No. 5,349,124), soybean having resistance to Lepidoptera (see, forexample, U.S. Ser. No. 7,432,421), and rice having resistance toLepidoptera (for example, Chilo suppressalis, Parnara guttata, Sesamiainferens, Cnaphalocrocis medinalis, rice caseworm, and rice armyworm)(see, for example, WO2001021821). The methods of manufacturing suchplants are generally well-known to a person skilled in the art and theseplants are disclosed in the above publications.

As plants to which pest resistance is imparted by RNA interferencetechnologies, corn resistant to Lepidoptera insect pests (for example,corn borers, cutworms such as corn earworms and black cutworms, and fallarmyworms) and Coleoptera insect pests (corn rootworms) is commerciallyavailable or is developed under the names of “SmartStax (registeredtrademark)”, “SmartStax (registered trademark) Pro”, “Genuity(registered trademark) SmartStax”.

Examples of plants to which pest resistance is imparted classically orby genome breeding technologies include aphid resistant soybean having aRagl (Resistance Aphid Gene 1) gene; soybean having resistance to Cystonematode; cotton having resistance to Root Knot nematode; rice “KantoETH No.1” having resistance to Nilaparvata lugens; and soybean“FUKUNOMINORI” having resistance to Spodoptera litura.

Resistance to optional insect pests (especially Lepidoptera insects,Coleoptera insects, and Diptera insects), noxious arachnids, and noxiousnematodes are imparted to plants to which resistance to these pests isimparted. Plants to which pest resistance is imparted are preferablyselected from cereal crops (for example, wheat, barley, rye, and oat);corn, canola, sorghum, soybean, rice, rape seed, sugar beet, sugarcane,grape, lentil, sunflower, alfalfa, kernel fruits; stone fruits; peanuts;coffee; tea; strawberries; turf grass; vegetables (for example, tomato,potato, plants of the family Cucurbitaceae, and lettuce), morepreferably selected from soybean, corn, tomatoes, rice, and cereal crops(for example, wheat, barley, rye, and oat), and even more preferablyselected from soybean, rice, corn, and cereal crops (for example, wheat,barley, rye, and oat).

Examples of the plants to which disease tolerance is imparted are givenbelow.

Plants to which disease tolerance is imparted by genetic recombinationtechnologies are those expressing so-called “pathogen related proteins”(PRP, see, for example, EP0392225) or so-called “antifungal proteins”(AFP, see, for example, U.S. Pat. No. 6,861,068). Various antifungalproteins having activity to plant pathogenic fungi are isolated fromspecific plants and become commonly used. Examples of such pathogenicsubstances and plants enabling synthesis of these plant pathogenicsubstances are well known from EP0392225, WO1993/05153, WO1995/33818,and EP0353191. Plants resistant to fungicidal pathogens, viralpathogens, and bacterial pathogens are produced by introducing plantresistant genes. Many resistant genes are identified and isolated, andthen, used to improve plant resistance. However, examples of theresistant genes include N genes (see, for example U.S. Pat. No.5,571,706) introduced into a type of tobacco sensitive to TMV to producea tobacco plant resistant to tobacco mosaic virus (TMV), Prf genes (see,for example, WO 199802545) introduced into a plant to obtainstrengthened pathogenic resistance, and Rps2 genes (see, for example, WO199528423) derived from thale cress (Arabidopsis thaliana) and used tocreate resistance to bacterial pathogens such as Pseudomonas syringae.Plants exhibiting systematic acquired resistance response was obtainedby introducing a nucleic acid molecule encoding the TIR domain of Ngenes (see, for example, U.S. Pat. No. 6,630,618). Further examples ofknown resistant genes include Xa21 genes (see, for example, U.S. Pat.Nos. 5,952,485, 5,977,434, WO1999/09151, and WO1996/22375) introducedinto many rice varieties, Rcgl genes (see, for example, US2006/225152)and prp1 genes (see, for example, U.S. Pat. No. 5,859,332 and WO2008017706) for Colletotrichum resistance, ppv-cp genes (see, forexample, US PP15,154Ps) and P1 genes (see, for example, U.S. Pat. No.5,968,828) to introduce resistance to Plum pox virus, genes such asBlb1, Blb2, Blb3, RB2, and Rpi-vnt1 (see, for example, US7148397) andLRPKml genes (see, for example, WO1999064600) to introduce resistance tophytophthora infestans in potato, P1 genes (see, for example, U.S. Pat.No. 5,968,828) and HA5-1 genes (see, for example, U.S. Pat. Nos.5,877,403 and 6,046,384) for resistance to potato virus Y, PIP genes(see, for example, EP0707069) to introduce a wide range of resistance toviruses such as potato virus X (PVX), potato virus Y (PVY), and potatoleafroll virus (PLRV), and genes such as NI16 genes, ScaM4 genes, andScaMS genes (see, for example, U.S. Pat. No. 6,706,952 and EP1018553) ofthale cress (Arabidopsis) to obtain fungus resistance. Common beanshaving resistance to Bean golden mosaic virus (hereinafter referred toas BGMV) are plants to which the resistance is imparted by RNAinterference (hereinafter referred to as RNAi) technologies and in whichdouble stranded RNA genes (sense and antisense acl genes) of areplication protein are introduced to inhibit the synthesis ofreplication protein of BGMV, thereby exhibiting resistance to BGMV. Themethods of manufacturing such plants are generally well-known to aperson skilled in the art and are described in the above publications.

Examples of antipathogenic substances which can be expressed by theseplants include ion channel blockers (for example, a sodium channelblocker and calcium channel blocker), for example, a viral KP1, KP4, orKP6 toxin; stilbene synthase; bibenzyl synthase; chitinase; glucanase;so-called “pathogenic related protein” (PRP: see, for example,EP0392225); antipathogenic substances produced by microorganisms, forexample, peptide antibiotics or heterocyclic antibiotics (see, forexample, WO1995/33818) or a protein factor or polypeptide factor(so-called “plant disease resistant genes” described in WO2003/000906)relating to plant pathogen protection.

Antipathogenic substances produced by plants can protect the plants fromvarious pathogens such as true fungi, virus, and bacteria. Useful plantsarousing increased interest in relation to the present invention areselected from cereal crops (for example, wheat, barley, rye, and oat),soybean, corn, rice, rape seed, kernel fruits, stone fruits, peanut,coffee, tea, strawberry, and turf grass, plants and vegetables (forexample, tomato and potato), cucurbits, papaya, melon, lenses, andlettuce, preferably from soybean, tomato, rice, and cereal crops (forexample, wheat, barley, rye, and oat), and more preferably from soybean,rice, and cereal crops (for example, wheat, barley, rye, and oat).

Examples of Plants having resistance to fungal pathogens include soybean(see, for example, WO 2008017706) having resistance to Asian soybeanrust; solanaceous plants such as cotton, tomato, and potato (see, forexample, U.S. Pat. Nos. 5,859,332, 7,148,397, and EP1334979) havingresistance to phytophthora infestans; corn (see, for example,US2006/225152) having resistance to the genus Colletotrichum such asColletotrichum graminicola, apple (see, for example, WO1999064600)having resistance to venturia inaequalis; plants, for example, rice,wheat, barley, rye, corn, oat, potato, melon, soybean, sorghum (see, forexample, U.S. Pat. No. 6,646,184 and EP1477557) having resistance tofusarium diseases (for example, fusarium graminearum, fusariumsporotrichioides, fusarium lateritium, fusarium pseudograminearum,fusarium sambucinum, fusarium culmorum, fusarium poae, fusariumacuminatum, and fusarium equiseti); and plants such as corn, soybean,cereal crops (particularly, wheat, rye, barley, oat, rye, and rice),tobacco, sorghum, sugarcane, and potato (see, for example, U.S. Pat.Nos. 5,859,332, 5,689,046, 6,706,952, EP1018553, and U.S. Pat. No.6,020,129), which have a wide range of fungicidal resistance.

Plants having resistance to bacterial pathogens and plants covered bythe present invention include rice having resistance to xylellafastidiosa (see, for example, U.S. Pat. No. 6,232,528); plants such asrice, cotton, soybean, potato, sorghum, corn, wheat, barley, sugarcane,tomato, and pepper (see, for example, WO2006/42145, U.S. Pat. Nos.5,952,485, 5,977,434, WO1999/09151, and WO1996/22375), which haveresistance to bacterial blight; and tomato (see, for example, Can. J.Plant Path., 1983, 5:251-255) having resistance to pseudomonas syringae.

Examples of plants having resistance to viral pathogens include kernelfruits (for example, plum, almond, apricot, cherry, peach, andnectarine) having resistance to Plum pox virus (PPV, see, for example,US PP15154Ps and EP0626449); potato (see, for example, U.S. Pat. No.5,968,828) having resistance to potato virus Y; plants such as potato,tomato, cucumber, and leguminous plants, which have resistance to tomatospotted with virus (TSWV, see, for example, EP0626449 and U.S. Pat. No.5,973,135); corn having resistance to corn streak disease virus (see,for example, U.S. Pat. No. 6,040,496); papaya having resistance topapaya ringspot virus (PRSV, see, for example, U.S. Pat. Nos. 5,877,403and 6,046,384); plants of the family Cucurbitaceae (for example,cucumber, melon, watermelon, and pumpkin) and solanaceous plants (forexample, potato, tobacco, tomato, eggplant, paprika, capsicum, andpepper), which have resistance to cucumber mosaic virus (CMV, see, forexample, U.S. Pat. No. 6,849,780); plants of the family Cucurbitaceae(for example, cucumber, melon, watermelon, and pumpkin) (see, forexample, U.S. Pat. No. 6,015,942), which have resistance to watermelonmosaic potyvirus 2 (WMV2) and zucchini yellow mosaic potyvirus (ZYMV);potato having resistance to potato leafroll virus (PLRV, see, forexample, U.S. Pat. No. 5,576,202); potato (see, for example, EP0707069)having a wide range of resistance to virus such as potato virus X (PVX),potato virus Y (PVY), and potato leafroll virus (PLRV); and common bean(see, for example, Mol Plant Microbe Interact. 2007 Jun.; 20(6):717-26.)having resistance to Bean golden mosaic virus.

There are plants having resistance to antibiotics (for example,kanamycin, neomycin, and ampicillin). A naturally derived bacteriumnptll gene expresses an enzyme that blocks the effect of antibioticskanamycin and neomycin. A gene ampR (also known as blaTEM1) that is anampicillin resistant gene is derived from bacteria Salmonella paratyphiand is used as a marker gene in the transformation of microorganisms andplants. The ampR participates in the synthesis of p-lactamase that is anenzyme which neutralizes antibiotics of a penicillin group includingampicillin. Examples of plants having resistance to antibiotics includepotato, tomato, flax, canola, rape seed, cole seed, and corn (see, forexample, Plant Cell Reports, 20, 2001, 610-615, Trends in Plant Science,11, 2006, 317-319, Plant Molecular Biology, 37, 1998, 287-296, Mol GenGenet., 257, 1998, 606-13. Plant Cell Reports, 6, 1987, 333-336, FederalRegister (USA), vol 60, No.113, 1995, P31139, Federal Register (USA),vol 67, No.226, 2002, P70392, Federal Register (USA), vol 63, No.88,1998, P25194, Federal Register (USA), vol 60, No.141, 1995, P37870,Canadian Food Inspection Agency, FD/OFB-095-264-A, October, 1999, andFD/OFB-099-127-A, October, 1999). The above plants are selectedpreferably from soybean, tomatoes, and cereal crops (for example, wheat,barley, rye, and oat) and most preferably from soybean and cereal crops(for example, wheat, barley, rye, and oat).

As available plants to which resistance to plant virus is imparted,there are products including papaya “Rainbow”, “SunUp”, and Huanong No.1″ to which resistance to Papaya ringspot virus is imparted; potato“Innate (registered trademark) Hibernate”, “Innate (registeredtrademark) Glaciate”, and “Innate (registered trademark) Acclimate”having resistance to phytophthora infestans; and potato “Newleaf(trademark)” having resistance to potato virus Y and/or potato leafrollvirus (PLRV).

Examples of plants to which disease tolerance is imparted classically orby genome breeding technologies include rice to which tolerance to blast(Magnaporthe oryzae) is imparted; rice to which tolerance to sheathblight disease (Rhizoctonia solani) is imparted; wheat to whichtolerance to red rust (Puccinia triticina) is imparted; wheat to whichtolerance to yellow rust (Puccinia striiformis f. sp. tritici) isimparted; wheat to which tolerance to black rust (Puccinia graminis f.sp. tritici) is imparted; wheat to which tolerance to powdery mildew(Blumeria graminis f. sp. tritici) is imparted; wheat to which toleranceto speckled leaf blotch (Zymoseptoria tritici) is imparted; wheat towhich tolerance to glume blotch (Stagonospora nodorum) is imparted;wheat to which tolerance to yellow spot (Pyrenophora tritic-repentis) isimparted; barley to which tolerance to powdery mildew (Blumeria graminisf. sp. hordei) is imparted; barley to which tolerance to small rust(Puccnia hordei) is imparted; barley to which tolerance to net blotch(Pyrenophora teres) is imparted; barley to which tolerance to scald(Rhynchosporium commune) is imparted; barley to which tolerance toRammularia disease (Ramularia collo-cygni) is imparted; corn to whichtolerance to anthrax (Anthracnose stalk rot) is imparted; corn to whichtolerance to Gray leaf spot disease (Gray leaf spot) is imparted; cornto which tolerance to bacterial blight (Goss's wilt) is imparted; cornto which tolerance to foot rot (Fusarium stalk rot) is imparted; soybeanto which tolerance to Asian soybean rust is imparted; soybean to whichtolerance to soybean stem and root blight (phytophthora rot) isimparted; soybean to which tolerance to Sudden death syndrome isimparted; pepper to which tolerance to plague (Phytophthora) isimparted; lettuce to which tolerance to powdery mildew is imparted;tomato to which tolerance to bacterial wilt is imparted; tomato to whichtolerance to Geminivirus is imparted; lettuce to which tolerance toDowny mildew is imparted; cruciferous plants such as rape seed, cabbage,brussels sprout, cauliflower, collard greend (Borekale), and broccoli towhich tolerance to Clubroot is imparted; cruciferous plants such as rapeseed, cabbage, brussels sprout, cauliflower, collard greend (Borekale),and broccoli to which tolerance to Black leg is imparted; and melon towhich tolerance to melon fusarium wilt caused by Fusarium oxysporumf.sp. melonis is imparted (see, for example, WO2009000736).

Examples of plants to which disease tolerance is imparted classically orby genome breeding technologies include bread wheat (see, for example,Nat. Biotech.,32,947-951, 2014) made resistant to powdery mildew byusing TALEN and CRISPR/Cas9 to delete powdery mildew resistant genes(MILDEW RESIST=LOCUS 0, hereinafter abbreviated as MLO); slmlol tomato(Tomelo) (see, for example, Scientific Reports 7, Article number: 482,2017) made tolerant to powdery mildew by using CRISPR/Cas9 to delete aS1MLC1 gene that is one of MLO; rice (see, for example, Nat.Biotechnol.30, 390-392, 2012) made tolerant to Xanthomonas oryzae pv. Oryzaecausing rice bacterial leaf blight by using TALEN to undergo edition ofOsSWEET 14 genes in rice; rice (see PLoS ONE 11:e0154027. doi:10.1371/journal.pone.0154027, 2016) made tolerant to Magnaporthe oryzaecausing rice blast by using CRISPR/Cas9 to modify OsERF922 genes inrice; cucumber (see Mol. PlantPathol. 17, 7 1140-1153, 2016) madetolerant to cucumber vein yellow ingvirus(CVYV), zucchini yellow mosaicvirus(ZYMV), and papaya ringspot virus-typeW (PRSV-W) by usingCRISPR/Cas9 to degrade a recessive eIF4E (eukaryote translationinitiation factor 4E) gene; and soybean (see Mol Plant Pathol17(1)127-39, 2016) made tolerant to Phytophthora root and stem rotcaused by Phytophthora sojae, by using CRIPR/Cas9 to degrade RXLReffecter genes (Avr4/6).

Examples of plants to which disease tolerance is imparted by newbreeding technologies include apple (see, for example, Plant Biotech.J.,12, 2-9, 2014) into which Rvi6 (previously called HcrVf2) gene havingtolerance to Apple scab caused by Venturia inaequalis is introduced byusing cisgenesis and which has tolerance to Apple scab; and sweet cherry(see, Plant Biotech. J., 12, 1319-1328, 2014) prepared by grafting inwhich a transgenic rootstock having tolerance to the infection of Prunusnecrotic ringspot virus transfers its tolerant nature to a nontransgenicscion as an example of transferring the nature of a rootstock to a scionin breeding technologies utilizing grafting.

Example of plants in which contents in these plants are modified aregiven below.

The modification of contents in a plant implies increase and decrease insynthesis of modified compounds or synthetic amount of chemicalsubstances as compared with the corresponding wild-type plants. Thereare, for example, modified plants increased or decreased in the contentsof vitamins, amino acids, proteins, and starch, and various oils andmodified plants reduced in nicotine content.

Examples of plants modified in content by genetic recombinationtechnologies include alfalfa reduced in lignin content by RNAinterference effects produced by introducing a double strand RNA ofS-adenosyl-L-methionine: trans-caffeoyl CoA-methyltransferase (ccomt)gene derived from alfalfa relating to lignin production; canola“Laurical (trademark) Canola” increased in the content oftriacylglyceride containing lauric acid by introducing 12 : 0 ACPthioesterase derived from laurier (Umbellularia californica) relating tofatty acid synthesis; soybean “Plenish (trademark)” or “Treus(trademark)” increased in oleic acid content through reduction of geneexpression by introducing a partial gene sequence (gm-fad2-1) of ω-6desaturase which is an unsaturated enzyme of fatty acid and is derivedfrom soybean; Soybean “Vistive Gold (trademark)” reduced in fatty acidcontent by introducing a gene creating a double strand DNA of anacyl-acyl carrier-protein-thioesterase gene (fatbl-A) derived fromsoybean and a gene creating a double strand DNA of a δ-12 desaturasegene (fad2-1A) derived from soybean; genetically modified soybeanincreased in the content of ω3 fatty acid by introducing a δ-6desaturase gene (Pj.D6D) derived from primrose and δ-12 desaturase gene(Nc. Fad3) derived from red bread mold; corn “Enogen (registeredtrademark)” increased in productivity of bioethanol by introducing aheat resistant α-amylase gene (amy797E) of Thermococcales sp. relatingto amylolysis; corn “Mavera (trademark) Maize” and “Mavera (trademark)YieldGard (trademark) Maize” increased in productivity of lysin byintroducing a dihydrodipicolinate synthetase gene (cordapA) derived fromCorynebacterium glutamicum relating to the production of lysin that isan amino acid; potato “Amflora (trademark)” and “Starch Potato”decreased in amylose content and increased in amylopectin content instarch particles by introducing an antisense gene gbss of starchsynthetase (granule-bound starch synthase enzame, GBSS) derived frompotato; potato “Innate (registered trademark) Cultivate”, “Innate(registered trademark) Generate”, “Innate (registered trademark)Accelerate”, “Innate (registered trademark) Invigorate”, “Innate(registered trademark) Glaciate”, “Innate (registered trademark)Acclimate”, and “Innate (registered trademark) Hibernate” reduced inamylolysis by introducing genes pPhL and pR1 creating a double strandRNA of transcription factor genes PhL and R1 promoting amylolysis andderived from potato, reduced in the synthesis of asparagine (for thepurpose of reducing the accumulation of asparagine and reducing sugarrelating to the generation of acrylamide that is a carcinogenicsubstance and is generated by heating), and reduced in black spotformation by introducing a gene PPO5 creating a double strand RNA ofpolyphenol oxidase gene PPO5 derived from potato; tobacco reduced innicotine content by introducing an antisense gene (NtQPT1) ofquinolinate phosphoribosyltransferase QpTase derived from tobacco(Nicotiana tabacum); and Golden rice that is a rice plant whichproduces) β-carotene in its albumen tissue and can harvest ricecontaining vitamin A by introducing a phytoene synthetase gene (psy)derived from daffodil (Narcissus pseudonarcissus) andcarotene-desaturase gene (crtl) derived from soil bacteria (Erwiniauredovora) synthesizing carotenoid to express in a way specific toalbumen. Other examples of plants modified in content by geneticrecombination technologies include potato and corn (see, for example,U.S. Pat. No. 6,784,338, US20070261136, and WO199704471) modified inamylopectin content; canola, corn, cotton, grape, cattail, catalpa,rice, soybean, rape seed, wheat, sunflower, bitter gourd, safflower, andvernonia plants (see, for example, U.S. Pat. Nos. 7,294,759, 7,157,621,5,850,026, 6,441,278, 5,723,761, 6,380,462, 6,365,802, 6,974,898,WO2001079499, US20060075515, and U.S. Pat. No. 7,294,759), which aremodified in oil content; sunflower (see, for example, U.S. Pat. No.6,084,164) increased in in fatty acid content; soybean (see, forexample, U.S. Pat. No. 6,864,362) decreased in allergen content; tobacco(see, for example, US20060185684, WO2005000352, and WO2007064636)reduced in nicotine content; canola and soybean (see, for example,Bio/Technology 13, 1995, 577-582) increased in lysin content; corn andsoybean (see, for example, U.S. Pat. No. 6,946,589 and U.S. Pat. No.6,905,877) modified in the compositions of methionine, leucin,isoleucine, and valine; soybean (see, for example, EP0929685 andWO1997041239) increased in the content of sulfur amino acid; tomato(see, for example, U.S. Pat. No. 6,727,411) increased in the content offree amino acid (for example, asparagine, aspartic acid, serine,threonine, alanine, histidine, and glutamic acid); corn (see WO05077117)increased in amino acid content; potato, corn, and rice (see, forexample, WO1997044471 and U.S. Pat. No. 7,317,146) modified in starchcontent; tomato, corn, grape, alfalfa, apple, pulses, and pea (see, forexample, WO0004175) modified in flavonoid content; corn, rice, sorghum,cotton, and soybean (see, for example, US20080235829) modified in theamount of phenolic compound; tomato and canola (see, for example, U.S.Pat. Nos. 6,797,498 and 7,348,167) increased in the content of vitaminA; tomato, canola, soybean, wheat, sunflower, rice, corn, barley, andrye (see, for example, U.S. Pat. No. 7,348,167 and WO2004058934)increased in the amount of vitamin E; and alfalfa, apple, bean, corn,grape, tomato, and pea (see, for example, WO0004175) modified inflavonoid content. The methods of manufacturing such plants aregenerally well-known to a person skilled in the art and these plantsare, for example, disclosed in the above publications. These plants arepreferably selected from soybean, canola, tomatoes, rice, and cerealcrops (for example, wheat, barley, rye, and oat) and more preferablyfrom soybean, canola, rice, wheat, and barley.

As plants modified in content either classically or by genome breedingtechnologies, rape seed “Nexera (registered trademark) Canola” producingunsaturated ω-9 fatty acid; soybean “Yumeminori” reduced in allergencontent; and rice developed with the intension of improving taste, forexample, rice “Yumepirika” reduced in amylose content are commerciallyavailable. Also, citrus fruits modified in fruit characteristics(weight, amount of fragrance, succulence, and sugar content of fruit) bygenomic selection (see, Scientific Reports 7, 4721, 2017).

Plants modified in plant nutrient utilization are those improved inassimilation or metabolization of nitrogen or phosphorus. Plants havingnitrogen assimilation ability and nitrogen utilization ability enhancedby genetic recombination technologies are selected from canola, corn,wheat, sunflower, rice, tobacco, soybean, cotton, alfalfa, tomato,wheat, potato, sugar beet, sugarcane, and rape seed (see, for example,WO1995009911, WO1997030163, U.S. Pat. Nos. 6,084,153, 5,955,651, and6,864,405). Plants improved in phosphorous uptake by geneticrecombination technologies include alfalfa, barley, canola, corn,cotton, tomato, rape seed, rice, soybean, sugar beet, sugarcane,sunflower, wheat, and potato (see, for example, U.S. Pat. No. 7,417,181and US 20050137366). The methods of manufacturing such plants aregenerally known to a person skilled in the art and these plants are, forexample, disclosed in the above publications. These plants arepreferably selected from soybean, tomato, and cereal crops (for example,wheat, barley, rye, and oat) and more preferably from soybean, rice,corn, and wheat.

As plants modified in fertility trait and the like by geneticrecombination technologies, plants to which male sterility and fertilityrestoring traits are imparted are exemplified. Examples of these plantsinclude corn and chicory to which a male sterility trait is imparted byexpressing a ribonuclease gene (barnase) derived from Bacillusamyloliquefaciens in tapetum cells of an anther; corn to which malesterility trait is imparted by introducing a DNA adeninemethylase gene(dam) derived from Escherichia coli; corn controlled in fertility traitby introducing an α-amylase gene (zm-aal) derived from corn imparting amale sterility trait and a ms45 protein gene (ms45) derived from cornimparting a fertility restoring trait; canola to which fertilityrestoring ability is imparted by expressing a ribonuclease inhibitoryprotein gene (barstar) derived from Bacillus in tapetum cells of ananther; and canola controlled in a fertility trait by expressing aribonuclease gene (barnase) derived from Bacillus imparting a malesterility trait and a ribonuclease inhibitory protein gene (barstar)derived from Bacillus imparting a fertility restoring trait. Otherexamples of plants to which a fertility trait is imparted by geneticrecombination technologies include tomato, rice, Indian mustard, wheat,soybean, and sunflower (see, for example, U.S. Pat. Nos. 6,720,481,6,281,348, 5,659,124, 6,399,856, 7,345,222, 7,230,168, 6,072,102,EP1135982, WO2001092544, and WO1996040949). The methods of manufacturingsuch plants are generally known to a person skilled in the art and theseplants are, for example, disclosed in the above publications. Theseplants are preferably selected from corn, canola, soybean, tomatoes, andcereal crops (for example, wheat) and most preferably from corn, canola,soybean, rice, and wheat.

Plants to which non-biological stress tolerance is imparted are thoseincreased in tolerance to nonbiological stress condition such asdrought, high salt content, high light intensity, high UV irradiation,chemical contamination (for example, high concentrations of heavymetals), low or high temperature, limited supply of nutrients, andcollective stress (see, for example, WO200004173, WO2007131699,CA2521729, and US20080229448).

Examples of plants to which non-biological stress tolerance is impartedinclude rice, corn, soybean, sugarcane, alfalfa, wheat, tomato, potato,barley, rape seed, bean, oak, sorghum, and cotton, which have toleranceto drought (see, for example, WO2005048693, WO2008002480, and WO2007030001); corn, soybean, wheat, cotton, rice, rape seed, and alfalfa,which have tolerance to low temperature (see, for example, U.S. Pat. No.4,731,499 and WO2007112122); and rice, cotton, potato, soybean, wheat,barley, rye, sorghum, alfalfa, grape, tomato, sunflower, and tobacco,which have tolerance to high salt content (see, for example, U.S. Pat.Nos. 7,256,326, 7,034,139, and WO/2001/030990). Examples of these plantsalso include corn “DroughtGard (registered trademark)” (product fromMonsanto) into which a cold shock protein gene cspB of Bacillus subtilisis introduced.

As the plants to which abiotic stress tolerance is imparted eitherclassically or by genetic recombination technologies, for example, cornhaving drought tolerance are developed under the trade names of“Agrisure Artesian (registered trademark)” and “Optimum (registeredtrademark) AQUAmax (trademark)”.

The modifications of maturation characteristics are, for example,delayed maturation, delayed softening, and acceleration of maturation.Examples of plants modified in maturation characteristics by geneticrecombination technologies include melon and tomato improved in shelflife by introducing a S-adenosylmethionine hydrolase gene (sam-K)derived from colon bacillus bacteriophage T3 relating to generation ofethylene of a plant hormone, tomato improved in shelf life byintroducing a partially deficient ACC synthetase gene derived fromtomato relating to generation of ethylene of a plant hormone, an Accdeaminase gene derived from Pseudomonas chlororaphis which degrades ACCthat is an ethylene precursor, a gene generating a double strand RNA ofa polygalacturonase gene which is derived from tomato and degrades cellwall pectin, or ACC oxidase gene derived from tomato relating togeneration of ethylene, and tomato “FLAVR SAVR (trademark)” improved inshelf life by introducing a gene pg generating a double strand RNA of apolygalacturonase gene derived from tomato. Other examples of plantsmodified by genetic recombination technologies include tomato, melon,raspberry, strawberry, mask melon, pepper, and papaya which are eachproduced by way of delayed maturation (see, for example, U.S. Pat. Nos.5,767,376, 7,084,321, 6,107,548, 55,981,831, WO1995035387, U.S. Pat.Nos. 5,952,546, 5,512,466, WO1997001952, WO1992/008798, Plant Cell.1989, 53-63. Plant Molecular Biology, 50, 2002). The methods ofmanufacturing such plants are generally known to a person skilled in theart and these plants are, for example, disclosed in the abovepublications. These plants are preferably selected from fruits (tomato,climbing plants, melon, papaya, banana, pepper, raspberry, andstrawberry); stone fruits (for example, cherry, apricot, and peach);kernel fruits (for example, apple and European pear); and citrus fruits(for example, citron, lime, orange, shaddock, grape fruits, andmandarin) and more preferably from tomato, melon, papaya, climbingplants, apple, banana, orange, and strawberry, and tomato, melon, andpapaya are most preferable.

Examples of plants modified in other qualities by genetic recombinationtechnologies include canola “Phytaseed (registered trademark) Canola”improved in the degradation of endogenous phytic acid by introducing a3-phytase gene (phyA) derived from Aspergillus niger that is an enzymethat breaks down plant phytic acid; carnation “Moondust (trademark)”,“Moonshadow (trademark)”, “Moonshade (trademark)”, “Moonlite(trademark)”, “Moonaqua (trademark)”, “Moonvista(trademark)”, “Moonique(trademark)”, “Moonpearl(trademark)”, “Moonberry (trademark)”, and“Moonvelvet (trademark)” controlled to change each flower color to blueby introducing a dihydroflavonol-4-reductase gene derived from petunia(Petunia hybrida) that is an enzyme producing delphinidin that is a bluepigment and its derivative or a flavonoid-3′,5′-hydroxylase gene derivedfrom petunia, pansy (Viola wittrockiana), salvia (Salvia splendens), orcarnation; rose controlled to change each flower color to blue byintroducing an anthocyanin-5-acyltransferase gene derived from torenia(Torenia sp.) that is an enzyme producing delphinidin that is a bluepigment and its derivative and a flavonoid-3,5′-hydroxylase gene derivedfrom pansy; genetically modified rice having pollinosis deactivationeffect associated with an immunotolerance action by introducing anantigen protein gene (7crp) of modified cedar pollen; corn improved inthe degradation of endogenous phytic acid by introducing a 3-phytasegene (phyA) derived from Aspergillus niger; and cotton producinghigh-quality fibers improved in fiber micronaire, fiber strengthincrease, length uniformity, and color (see, for example, WO 1996/26639,U.S. Pat. Nos. 7,329,802, 6,472,588, and WO 2001/17333).

Examples of plants modified in trait relating to plant growth and yieldinclude plants improved in growing ability. As plants modified bygenetic recombination technologies, soybean has been developed which isimproved in plant growth with the expectation of resultant high yield byintroducing a gene (bbx32) encoding a transcription factor controllingdaily periodicity specific to thale cress; and corn has been alsodeveloped which is increased in female panicle weight with theexpectation of resultant high yield by introducing a transcriptionfactor gene (athb17) belonging to homeodomain-leucine 14 zipper (HD-Zip)family, class II (HD-Zip II) derived from thale cress.

Examples of plants modified in quality by genome editing technologiesinclude corn “ZFN-12 maize” reduced in phytic acid content by using zincfinger nuclease to delete an IPK1 gene encodinginositol-1,3,4,5,6-pentakisphosphate 2-kinase that is a phytic acidsynthetic enzyme; and mushroom to which browning tolerance is impartedby using CRISPR-Cas9 to delete a gene encoding a polyphenol oxidase(see, for example, Nature.,Vol 532, 21 Apr., 2016).

Examples of plants modified in quality by new breeding technologiesinclude apple “Arctic (registered trademark)” which is reduced inpolyphenol oxidase expression level and is resistant to browning byusing cisgenesis to introduce low polyphenol oxidase (enzyme causingbrowning) production gene sequence GEN-03 isolated from apples into anew apple variety; and an example (see, for example, Physiol Plantarum,124, 465-475, 2005) in which salt tolerance is imparted to anontransgenic tomato scion by using a tomato roctstock having salttolerance as an example in which the nature of a rootstock istransferred to a scion in variety improvement technologies utilizinggrafting.

With regard to rice, genes are known which have tolerance to manydiseases, noxious insects, and abiotic stress and resistant varietiesobtained by introducing these tolerance factors are enthusiasticallymade. As examples of genes having tolerance to rice diseases and abioticstress, Nilaparvata lugens resistant genes such as BPH1, BPH2, BPH3,BPH4, BPH5, BPH6, BPH7, BPH8, BPH9, BPH10, BPH11, BPH12, BPH13, BPH14,BPH15, BPH17, BPH18, BPH19, BPH2O, BPH21, BPH22, BPH23, BPH24, BPH25,BPH26, BPH27, BPH28, BPH29, BPH32, qBPH-12, qBPHR-1, qBPHR-3, qBPHR-8,qBPHR-5-1qBPHR-5-2qBPHR-11-1, qBPHR-11-2; Sogatella furcifera resistantspecies such as WBPH1, WBPH2, WBPH3, WBPH4, WBPH5, WBPH6, OVC, q0VA-5-2,q0VA1-3, q0VA5-1; Laodelphax striatella resistant genes such as Qsbph2b,Qsbph3, Qsbph3b, Qsbph3c, Qsbph3d, Qsbph4, Qsbph8, Qsbphll, Qsbph11d,Qsbph11e, Qsbph11f, Qsbph11g, Qsbph12a; sidewinder resistant genes suchas GLH, GLH1, GLH3, GLH4, GLH5, GLH6, GLH7, GLH8, GLH9, GLH10, GLH11,GLH12, GLH13, GRH1, GRH2, GRH3, GRH4, GRHS, Zlh1, Zlh2, Zlh3, _(q)GRH-4,qGRH-2, qGRH-5, qGRH-6, qGRH-11, _(q)GRH-3; Chilo suppressails resistantgenes such as SB; rice blast resistant genes such as Pii, PI65, PIZT,PI24, PI29, PI25, Pi-jnw1, PB1, PIQ6, PID3, PI67, PITQ5, PITP, PITQ6,PLM2, PISE3, IPI, PISE1, PI157, PIQ4, PI21, PIA, PIB, PIK, PIKUR1,PIKUR2, PI3, PIF, PIZH, PIR4, PIR7, PI30, PI, PIGD2, PIG, PIGD3, PIGD1,PIZ, PI(, PI18, PIM, PI17, PI20, PI1, PI19, PI5, PISH, PI10, P19, PI21,PI22, PI44, PI22, PI113, PII, PIB1, PIQ1, PIQ2, PIQ3, PIIS1, PII2, PI62,PI12, IPI3, PI14, PI15, PI16, PIT, PI11, PI6, PI23, PI14, PI11, PIIS2,PIB2, PI12, PI39, PI40, PITA, PIR2-3, PIR9-2, PIR12-2, PIRF2-1,_(q)RBR-2, _(q)RBR-3, PI27, PI28, PI26, PIGM, PI47, PI48, PI7, PI56,PI49, PI34, PIKG, P138, P132, P131, P146, PIX, PIXY, Pita3, P141, P142,PI2, PI36, PI37, PIKH, PIKM, PIKP, PI35, PIZ5, PIB2, PI43, PI50, PI51,PID1, PIY1, PIY2, PI55-2, PICC39, PI55-1, PIBH8, PIR7A, PIR7B, PID2,PI33, _(q)BFR4-1, _(q)BFR4-2, _(q)RBR-2, qRBR-3, qRBR-8, qRBR-1-1,_(q)RBR-1-3, _(q)RBR-7-1, _(q)RBR-7-2, qRBR-9-1, qRBR-9-2, qRBR-9-3,_(q)RBR-1-2, _(q)RBR-1-4; leaf stripe disease resistant genes such asSTVA, STVB, and Stvb-i; leaf bright resistant genes such as XA21D, XA,XA40, XA NM, XA8, XA33, XA34, XA35, XA36, XA37, XA7, XA3, XA25, XA28,XA29, XA30, XA31, XA32, XA38, XA39, XA11, XA16, XA17, XA18, XA19, XA20,XA14, XA2, XA12, XA1, XA K, XA A, XA H, XA10, XA23, XA22, XA24, XA21,SERRT13, XA4, XA5, XA13; sheath blight disease resistant genes such asqSB-2, qSB-3, qSB-7, qSB-11, and qSB-9-1; brown spot resistant genessuch as CE; yellow dwarf resistant genes such as YDV; black streakeddwarf resistant genes such as BSV; high-temperature ripening resistantgenes such as Amy1A, Amy1C, Amy3A, and Amy3B; low-amylose genes such asdu13, qAC9.3, rsrl, Wx, and Wx1-1; lodging resistant genes such as AP01,SCM2, and Sd1; sprouting resistant genes such as Sdr4; low-temperatureresistant genes such as CTBT, CTB2, and qLTG3-1; drought tolerant genessuch as Drol; genes such as DEPT, Cnla, GPS, SPIKE, PTB1, TAWAWA1, WFP,IPA1, GS3, GSS, GS6, GL3.1, GW2, GW8, qGL3, qSS7, and qSW5 which relateto chaff number or seed form; genes controllingphotoperiod-insensitivity such as Hd1, Ghd8, and DTH8; endospermmealiness genes such as FLO4 and PDIL1; lipoxygenase deletion (reducingold-rice smell) genes such as LOX3; and genes such as Alk relating toamylopectin chain length are known. Rice varieties into which one or twoor more of these genes are incorporated simultaneously are developed andput on the market.

The above plants include plant lines imparted with two or more naturesspecific to parent lines by crossing a line added with two or more ofthe properties like those mentioned above, for example, abiotic stresstolerance, disease tolerance, herbicide tolerance, pest tolerance,growth and yield traits, nutrient-uptake, product qualities, andsterility trait with a plant of the same kind, or with a plant havingdifferent natures by using genetic recombination technologies, classicalbreeding technologies, genome breeding technologies, new breedingtechnologies, or genome editing technologies.

Examples of commercially available plants to which tolerance to two ormore herbicides is imparted include cotton “GlyTol (trademark)LibertyLink (trademark)” and “GlyTol (trademark) LibertyLink(trademark)” having tolerance to glyphosate and glufosinate; corn“Roundup Ready (trademark) LibertyLink (trademark) Maize” havingtolerance to glyphosate and glufosinate; soybean “Enlist (trademark)Soybean” having tolerance to glufosinate and 2,4-D; soybean “Genuity(trademark) Roundup Ready (trademark) 2 Xtend (trademark)” havingtolerance to glyphosate and dicamba; corn and soybean “OptimumGAT(trademark)” having tolerance to glyphosate and ALS inhibitors;genetically modified soybean “Enlist E3 (trademark)” and “Enlist(trademark) Roundup Ready 2 Yield (trademark)” having tolerance to threeherbicides: glyphosate, glufosinate, and 2,4-D; genetically modifiedcorn “Enlist (trademark) Roundup Ready (registered trademark) Corn 2”having tolerance to glyphosate, 2,4-D, and allyloxyphenoxypropionatetype (FDPs) herbicides; genetically modified corn “Enlist (trademark)Roundup Ready (registered trademark) Corn 2” having tolerance toglyphosate, 2,4-D, and allyloxyphenoxypropionate type (FDPs) herbicides;genetically modified cotton “Bollgard II (registered trademark)XtendFlex (trademark) Cotton” having tolerance to dicamba, glyphosate,and glufosinate; and genetically modified cotton “Enlist (trademark)Cotton” having tolerance to three herbicides: glyphosate, glufosinate,and 2,4-D. Other than the above, cotton having tolerance to glufosinateand 2,4-D, cotton having tolerance to both glufosinate and dicamba, cornhaving tolerance to both glyphosate and 2,4-D, soybean having toleranceto both glyphosate and HPPD herbicides, and genetically modified cornhaving tolerance to glyphosate, glufosinate, 2,4-D,allyloxyphenoxypropionate type (FDPs) herbicides, and cyclohexadionetype (DIMS) herbicides are also developed.

Examples of commercially available products of plants to which herbicidetolerance and pest resistance are imparted include corn “YieldGardRoundup Ready” and “YieldGard Roundup Ready 2” having glyphosatetolerance and corn borer resistance; corn “Agrisure CB/LL” havingglufosinate tolerance and corn borer resistance; corn “Yield Gard VTRoot worm/RR2” having glyphosate tolerance and corn rootworm resistance;corn “Yield Gard VT Triple” having glyphosate tolerance and cornrootworm resistance and corn borer resistance; corn “Herculex I” havingglufosinate tolerance and Lepidoptera insect pest resistance (Cry1F)(resistance to, for example, a western bean cutworm, corn borer, blackcutworm, and fall armyworm); corn “YieldGard Corn Rootworm/Roundup Ready2” having glyphosate tolerance and corn root worm resistance; corn“Agrisure GT/RW” having glufosinate tolerance and Coleoptera insect pestresistance (Cry3A) (resistance to, for example, a western corn rootworm,northern corn rootworm, and Mexican corn rootworm); corn “Herculex RW”having glufosinate tolerance and Coleoptera insect pest resistance(Cry34/35Abl) (resistance to, for example, a western corn rootworm,northern corn rootworm, and Mexican corn rootworm); corn “Yield Gard VTRoot worm/RR2” having glyphosate tolerance and corn rootworm resistance;and cotton “Boligard 3 (registered trademark) XtendFlex (registeredtrademark)” having dicamba tolerance, glyphosate tolerance, glufosinatetolerance, and Lepidoptera insect pest resistance (resistance to, forexample, bollworms, tobacco budworm, and armyworms).

Examples of commercially available plants to which disease tolerance andpest resistance are imparted include potato “Hi-Lite NewLeaf (trademark)Y Potato”, “NewLeaf (trademark) Y Russet Burbank Potato”, and “ShepodyNewLeaf (trademark) Y Potato” to which potato virus Y tolerance and pestresistance are imparted; and potato “NewLeaf (trademark) Plus RussetBurbank Potato” to which potato leaf roll virus tolerance and pestresistance are imparted.

Examples of commercially available plants to which herbicide toleranceand modified product quality are imparted include canola “InVigor(trademark) Canola” to which glufosinate tolerance and fertility traitare imparted; corn “InVigor (trademark) Maize” to which glufosinatetolerance and fertility trait are imparted; and soybean “Vistive Gold(trademark)” modified in glyphosate tolerance and oil content.

Examples of commercially available plants having three or more traitsinclude corn “Herculex I/Roundup Ready 2” having glyphosate tolerance,glufosinate tolerance, and Lepidoptera insect pest resistance (Cry1F)(specifically, resistance to western bean cutworm, corn borer, blackcutworm, and fall armyworm); corn “YieldGard Plus/Roundup Ready 2”having glyphosate tolerance, corn rootworm resistance, and corn borerresistance; corn “Agrisure GT/CB/LL” having glyphosate tolerance,glufosinate tolerance, and corn borer resistance; corn “Herculex Xtra”having glufosinate tolerance, Lepidoptera insect pest resistance(Cry1F), and Coleoptera insect pest resistance (Cry34/35Ab1)(specifically, resistance to Lepidoptera insect pests such as a westernbean cutworm, corn borer, black cutworm, and fall armyworm andresistance to Coleoptera insect pests such as western corn rootworm,northern corn rootworm, and Mexican corn rootworm); corn “AgrisureCB/LL/RW” having glufosinate tolerance, corn borer resistance (Cry1Ab),and Coleoptera insect pest resistance (Cry3A) (specifically, resistanceto Coleoptera insect pests such as western corn rootworm, northern cornrootworm, and Mexican corn rootworm); corn “Agrisure (trademark) 3000GT”having glyphosate tolerance +corn borer resistance (Cry1Ab), andColeoptera insect pest resistance (Cry3A) (specifically, resistance towestern corn rootworm, northern corn rootworm, and Mexican cornrootworm); corn “Mavera high-value corn” having glyphosate tolerance,resistance to a corn rootworm and European corn borer, and a high lysinetrait; corn “Optimum (registered trademark) Leptra (trademark)” havingresistance to pests such as a European corn borer, southwestern cornborer, corn earworm, fall armyworm, black cutworm, and western beanwormcausing damages on the ground,

Soybean “Credenz (registered trademark) soybean” which is added withresistance to frogeye leaf spot, Sudden death syndrome, southern stemcanker, Phytophthora root rot, southern root-knot nematode, Scierotiniawhite mold, brown stem rot, and soybean cyst nematode, is improved iniron chlorosis, and is modified in chloride sensitivity, and cotton“Stoneville (registered trademark) Cotton” to which tolerance to aplurality of herbicides and pest resistance are imparted, while thereare nine cotton varieties ST5517GLTP, ST4848GLT, ST4949GLT, ST5020GLT,ST5115GLT, ST6182GLT, ST4747GLB2, ST4946GLB2, and ST6448GLB2 to copewith the situation of the outbreak of weeds and noxious insects on thefields in various districts.

Plants which are commercially available or are developed are listedbelow (A1 to A550). It is to be noted that the items in parenthesesindicate [Plant Name, Event Name, Event Code, Tradename] and NA means“No information” or “Unavailable information”. Most of these plants arelisted in the registered database (GM APPROVAL DATABASE) in theelectronic information site (http://www.isaaa.org/) of INTERNATIONALSERVICE for the ACQUISITION of AGRI-BIOTECH APPLICATIONS (ISAAA).

A1:[alfalfa, J101, MON-00101-8, Roundup Ready(trademark)Alfalfa],A2:[alfalfa, J101×J163, MON-00101-8×MON-00163-7, RoundupReady(trademark)Alfalfa], A3: [alfalfa, J163, MON-00163-7, RoundupReady(trademark)Alfalfa], A4:[alfalfa, KK179, MON-00179-5,HarvXtra(trademark)], A5: [alfalfa, KK179×J101, MON-00179-5×MON-00101-8], A6: [apple, GD743, OKA-NB001-8, Arctic (trademark)“Gclden Delicious”Apple], A7: [apple, GS784, OKA-NB002-9, Arctic (trademark)], A8: [apple,NF872, OKA-NB003-1, Arctic (trademark) Fuji Apple], A9: [Argentinecanola, 23-18-17 (Event 18), CGN-89111-8, Laurical(trademark) Canola],A10: [Argentine canola, 23-198 (Event 23), CGN-89465-2, Laurical(trademark) Canola], All: [Argentine canola, 61061, DP-061061-7], A12:[Argentine canola, 73496, DP-073496-4, Optimum (registered trademark)Gly canola], A13: [Argentine canola, 73496×RF3, DP-073496-4×ACS-BN003-6,NA], A14: [Argentine canola, GT200 (RT200), MON-89249-2, RoundupReady(trademark) Canola], A15: [Argentine canola, GT73 (RT73),MON-00073-7, Roundup Ready(trademark) Canola], A16: [Argentine canola,HCN10 (Topas 19/2), NA, Liberty Link(trademark)Independence(trademark)], A17: [Argentine canola, HCN28 (T45),ACS-BN008-2, InVigor(trademark) Canola], A18: [Argentine canola,HCN28×MON88302, ACS-BN008-2×MON-88302-9,InVigor(trademark)×TruFlex(trademark) Roundup Ready(trademark) Canola],A19: [Argentine canola, HCN92 (Topas 19/2), ACS-BN007-1, LibertyLink(trademark) Innovator(trademark)], A20: [Argentine canola,HCN92×MON88302, ACS-BN007-1×MON-88302-9, Liberty Link(trademark)Innovator(trademark)−TruFlex(trademark) Roundup Ready(trademark)Canola], A21: [Argentine canola, MON88302, MON-88302-9,TruFlex(trademark) Roundup Ready(trademark) Canola], A22: [Argentinecanola, MON88302×MS8×RF3, MON-88302-9×ACS-BN005-8×ACS-BN003-6,InVigor(trademark)×TruFlex(trademark) Roundup Ready(trademark) Canola],A23: [Argentine canola, MON88302×RF3, MON-88302-9×ACS-BN003-6, NA], A24:[Argentine canola, MPS961, NA, Phytaseed(trademark) Canola], A25:[Argentine canola, MPS962, NA, Phvtaseed(trademark) Canola], A26:[Argentine canola, MPS963, NA, Phytaseed(trademark) Canola], A27:[Argentine canola, MPS964, NA, Phytaseed(trademark) Canola], A28:[Argentine canola, MPS965, NA, Phytaseed(trademark) Canola], A29:[Argentine canola, MS1 (B91-4), ACS-BN004-7, InVigor(trademark) Canola],A30: [Argentine canola, MS1×MON88302, ACS-BN004-7×MON-88302-9,InVigor(trademark)×TruFlex(trademark) Roundup Ready(trademark) Canola],A31: [Argentine canola, MS1×RF1 (PGS1), ACS-BN004-7×ACS-BN001-4,InVigor(trademark) Canola], A32: [Argentine canola, MS1×RF1 (PGS2),ACS-BN004-7×ACS-BN002-5, InVigor(trademark) Canola], A33: [Argentinecanola, MS1×RF3, ACS-BN004-7×ACS-BN003-6, InVigor(trademark) Canola],A34: [Argentine canola, MS8, ACS-BN005-8, InVigor(trademark) Canola],A35:[Argentine canola, MS8×MON88302, ACS-BN005-8×MON-88302-9,InVigor(trademark)×TruFlex(trademark) Roundup Ready(trademark) Canola],A36:[Argentine canola, MS8×RF3, ACS-BN005-8×ACS-BN003-6,InVigor(trademark) Canola], A37: [Argentine canola, MS8×RF3×GT73 (R173),ACS-BN005-8×ACS-BN003-6×MON-00073-7, NA], A38:[Argentine canola,OXY-235, ACS-BN011-5, Navigator(trademark) Canola], A39: [Argentinecanola, PHY14, NA, NA], A40:[Argentine canola, PHY23, NA, NA], A41:[Argentine canola, PHY35, NA, NA], A42: [Argentine canola, PHY36, NA,NA], A43:[Argentine canola, RF1 (B93-101), ACS-BN001-4,InVigor(trademark) Canola], A44:[Argentine canola, RF1×MON88302,CS-BN001-4×MON-88302-9, InVigor(trademark)×TruFlex(trademark) RoundupReady(trademark) Canola], A45:[Argentine canola, RF2 (B94-2),ACS-BN002-5, InVigor(trademark) Canola], A46:[Argentine canola,RF2×MON88302, ACS-BN002-5×MON-88302-9, InVigor(trademark)×TruFlex(trademark) Roundup Ready(trademark) Canola],A47:[Argentine canola, RF3, ACS-BN003-6, InVigor(trademark) Canola],A48:[bean, EMBRAPA5.1, EMB-PV051-1, NA], A49:[carnation, 11(7442),FLO-07442-4, Moondust(trademark)], A50:[carnation, 11363(1363A),FLO-11363-1, Moonshadow(trademark)], A51:[carnation, 1226A(11226),FLO-11226-8, Moonshade(trademark)], A52:[carnation, 123.2.2(40619),FLO-40619-7, Moonshade(trademark)], A53:[carnation, 123.2.38(40644),FLO-40644-4, Moonlite(trademark)], A54:[carnation, 123.8.12,FLO-40689-6, Moonaqua(trademark)], A55:[carnation, 123.8.8(40685),FLO-40685-1, Moonvista(trademark)], A56:[carnation, 1351A(11351),FLO-11351-7, Moonshade(trademark)], A57:[carnation, 1400A(11400),FLO-11400-2, Moonshade(trademark)], A58:[carnation, 15, FLO-00015-2,Moondust(trademark)], A59:[carnation, 16, FLO-00016-3,Moondust(trademark)], A60:[carnation, 19907, IFD-19907-9,Moonl_(i)te(trademark)], A61:[carnation, 25947, IFD-25947-1,Moonpearl(trademark)], A62:[carnation, 25958, IFD-25958-3,Moonberry(trademark)], A63:[carnation, 26407, IFD-26407-2,Moonvelvet(trademark)], A64:[carnation, 4, FLO-00004-9,Moondust(trademark)], A65:[carnation, 66, FLO-00066-8,NA]A66:[carnation, 959A(11959), FLO-11959-3, Moonshade(trademark)],A67:[carnation, 988A(11988), FLO-11988-7, Moonshade(trademark)],A68:[chicory, RM3-3, NA, SeedLink(trademark)], A69:[chicory, RM3-4, NA,SeedLink(trademark)], A70: [chicory, RM3-6, NA, SeedLink(trademark)],A71 : [cotton, 19-51a, DD-01951A-7, NA], A72 : [cotton, 281-24-236,DAS-24236-5, NA], A73 : [cotton, 281-24-236×3006-210-23(MXB-13),DAS-24236-5×DAS-21023-5, WideStrike(trademark)Cotton], A74:[cotton,281-24-236×3006-210-23×COT102×81910,DAS-24236-5×DAS-21023-5×SYN-IR102-7×DAS-81910-7, NA], A75:[cotton,3006-210-23, DAS-21023-5, NA], A76:[cotton,3006-210-23×281-24-236×MON1445, DAS-21023-5×DAS-24236-5×MON-01445-2,WideStrike (trademark)RoundupReadv (trademark) Cotton], A77:[cotton,3006-210-23×281-24-236×MON88913,DAS-21023-5NA×NADAS-24236-5NA×NAMON-88913-8,Widestrike(trademark)RoundupReadyFlex (trademark)Cotton], A78:[cotton,3006-210-23×281-24-236×MON88913×COT102,DAS-21023-5NA×NADAS-24236-5NA×NAMON-88913-8NA×NASYN-IR102-7, Widestrike(trademark)RoundupReadyFlex (trademark)Cotton], A79:[cotton, 31707, NA,BXN (trademark)PlusBollgard (trademark)Cotton], A80:[cotton, 31803, NA,BXN (trademark)PlusBollgard (trademark)Cotton], A81:[cotton,31807×31808, NA, NA], A82:[cotton, 31807, NA,BXN(trademark)PlusBollgard(trademark)Cotton], A83:[cotton, 31808, NA,BXN (trademark)PlusBollgard (trademark)Cotton], A84:[cotton, 42317, NA,BXN (trademark)PlusBollgard (trademark)Cotton], A85:[cotton, 81910,DAS-81910-7, NA], A86:[cotton, BNLA-601, NA, NA], A87:[cotton,BXN10211(10211), BXN-10211-9, NA], A88:[cotton, BXN10215(10215),BXN-10215-4NA, BXN(trademark)Cotton], A89:[cotton, BXN10222(10222),BXN-10222-2, BXN(trademark)Cotton], A90:[cotton, BXN10224(10224),BXN-10224-4NA, BXN(trademark)Cotton], A91:[cotton, COT102(IR102),SYN-IR102-7, VIPCOT(trademark)Cotton], A92:[cotton, COT102×COT67B,SYN-IR102-7NA×NASYN-IR67B-1, VIPCOT (trademark) Cotton], A93:[cotton,COT102×COT67E×MON88913, SYN-IR102-7NA×NASYN-IR67B-1NA×NAMON-88913-8,VIPCOT(trademark)RoundupReadyFlex(trademark)Cotton], A94:[cotton,COT102×MON15985, SYN-IR102-7NA×NAMON-15985-7, Bollgard(registeredtrademark) III], A95:[cotton, COT102×MON15985×MON88913,SYN-IR102-7NA×NAMON-15985-7NA×NAMON-88913-8, Bollgard(registeredtrademark)III×RoundupReady(trademark)Flex(trademark)], A96:[cotton,COT102×MON15985×MON88913×MON88701,SYN-IR102-7NA×NAMON-15985-7NA×NAMON-88913-8NA×NAMONNA88701-3, NA],A97:[cotton, COT67B(IR67B), SYN-IR67B-1, NA], A98:[cotton, Event1, NA,JK1], A99:[cotton, GFMCry1A, GTL-GFM311-7, NA], A100:[cotton, GHB119,BCS-GH005-8, NA], A101:[cotton, GHB614, BCS-GH002-5, GlyTol(trademark)],A102:[cotton, GHB614×LLCotton25, BCS-GH002-5NA×NAACS-GH001-3,GlyTol(trademark)LibertyLink(trademark)], A103:[cotton,GHB614×LLCotton25×MON15985, BCS-GH002-5NA×NAACS-GH001-3NA×NAMON-15985-7,NA], A104:[cotton, GHB614×MON15985, BCS-GH002-5NA×NAMON-15985, NA],A105:[cotton, GHB614×T304-40×GHB119,BCS-GH002-5NA×NABCS-GH004-7NA×NABCS-GH005-8,Glytol(trademark)xTwinlink(trademark)], A106:[cotton,GHB614×T304-40×GHB119×COT102,BCS-GH002-5NA×NABCS-GH004-7NA×NABCS-GH005-8NA×NASYN-IR102-7,Glytol(trademark)×Twinlink(trademark)×VIPCOT(trademark)Cott on],A107:[cotton, GK12, NA, NA], A108:[cotton, LLCotton25, ACS-GH001-3NA,Fihermax(trademark)LihertyLink(trademark)], A109:[cotton,LLCotton25×MON15985, ACS-GH001-3NA×NAMON-15985-7,Fibermax(trademark)LibertyLink(trademark)BollgardII(tradema rk)],A110:[cotton, MLS9124, NA, NA], A111:[cotton, MON1076, MON-89924-2,Bollgard(trademark)Cotton], A112:[cotton, MON1445, MON-01445-2,RoundupReady(trademark)Cotton], A113:[cotton, MON15985, MON-15985-7,BollgardII(trademark)Cotton], A114:[cotton, MON15985×MON1445,MON-15985-7NA×NAMON-01445-2,RoundupReady(trademark)BollgardII(trademark)Cotton], A115:[cotton,MON1698, MON-89383-1, RoundupReady(trademark)Cotton], A116:[cotton,MON531, MON-00531-6, Bollgard(trademark)Cotton,Ingard(trademark)],A117:[cotton, MON531kMON1445, MON-0531-6NA×NAMON-01445-2,RoundupReady(trademark)Bollgard(trademark)Cotton], A118:[cotton, MON757,MON-00757-7, Bollgard(trademark)Cotton], R119:[cotton, MON88701,MONNA88701-3, NA], A120:[cotton, MON88701×MON88913,MONNA88701-3NA×NAMON-88913-8, NA], A121:[cotton,MON88701×MON88913×MON15985,MONNA88701-3NA×NAMON-88913-8NA×NAMON-15985-7, Bollgard II(registeredtrademark)×tendFlex(trademark)Cotton], R122:[cotton, MON88913,MON-88913-8, RoundupReady(trademark)Flex(trademark)Cotton],A123:[cotton, MON88913×MON15985, MON-88913-8NA×NAMON-15985-7,RoundupReady(trademark)Flex(trademark)BollgardII(trademark) Cotton],A124:[cotton, NgweChi6Bt, NA, NgweChi6Bt], A125:[cotton, SGK321, NA,NA], A126:[cotton, T303-3, BCS-GH003-6, NA], A127:[cotton, T304-40,BCS-GH004-7, NA], A128:[cotton, T304-40×GHB119,BCS-GH004-7NA×NABCS-GH005-8, TwinLink(trademark)Cotton], A129:[Creepingbentgrass, ASR368, SMG-36800-2, NA], A130:[eggplant, BtBrinjalEventEE1,BtBrinjalEventEE1, BARIBtBegun-1,-2,-Sand-4], A131:[eucalyptus, H421,H421, GMEucalyptus], A132:[flax, FP967(CDCTriffid), CDC-FLO01-2,CDCTriffidFlax], A133:[ lentil, RH44, RH44,Clearfield(trademark)lentil], A134:[corn, 32138, DP-32138-132138,2138SPTmaintainer], A135:[corn, 3272, SYN-E3272-5, Enogen(trademark)],A136:[corn, 3272×Bt11, SYN-E3272-5×SYN-BT011-1, NA], A137:[corn,3272×Bt11×GA21, SYN-E3272-5×SYN-BT011-1×MON-00021-9, NA], A138:[corn,3272×Bt11×MIR604, SYN-E3272-5×SYN-BT011-1×SYN-IR604-5, NA], A139:[corn,3272×BT11×MIR604×GA21, SYN-E3272-5×SYN-BT011-1×SYN-IR604-5×MON-00021-9,NA], A140:[corn, 3272×Bt11×MIR604×TC1507×5307×GA21,SYN-E3272-5×SYN-BT011-1×SYN-IR604-5×DAS-01507-1×SYN-05307-1×MON-00021-9,NA], A141:[corn, 3272×GA21, SYN-E3272-5×MON-00021-9, NA], A142:[corn,3272×MIR604, SYN-E3272-5×SYN-IR604-5, NA], A143:[corn, 3272×MIR604×GA21,SYN-E3272-5×SYN-IR604-5×MON-00021-9, NA], A144:[corn, 33121,DP-033121-3, NA], A145:[corn, 4114, DP-004114-3, NA], A146:[corn, 5307,SYN-05307-1, Agrisure(registered trademark)Duracade(trademark)],A147:[corn, 5307×GA21, SYN-05307-1×MON-00021-9, NA], A148:[corn,5307×MIR604×Bt11×TC1507×GA21,SYN-05307-1×SYN-IR604-5×SYN-BT011-1×DAS-01507-1×MON-00021-9,Agrisure(registered trademark)Duracade(trademark)5122], A149:[corn,5307×MIR604×Bt11×TC1507×GA21×MIR162,SYN-05307-1×SYN-IR604-5×SYN-BT011-1×DAS-01507-1×MON-00021-9×SYN-IR162-4,Agrisure(registered trademark)Duracade(trademark)5222], A150:[corn,59122, DAS-59122-7, Herculex(trademark)RW], A151:[corn, 59122×DAS40278,DAS-59122-7×DAS-40278-9, NA], A152:[corn, 59122×GA21,DAS-59122-7×MON-00021-9, NA], A153:[corn, 59122×MIR604,DAS-59122-7×SYN-IR604-5, NA], A154:[corn, 59122×MIR604×GA21,DAS-59122-7×SYN-IR604-5×MON-00021-9, NA], A155:[corn,59122×MIR604×TC1507, DAS-59122-7×SYN-IR604-5×DAS-01507-1, NA],A156:[corn, 59122×MIR604×TC1507×GA21,DAS-59122-7×SYN-IR604-5×DAS-01507-1×MON-00021-9, NA], A157: [corn,59122×MON810, DAS-59122-7×MON-00810-6, NA], A158:[corn,59122×MON810×MIR604, DAS-59122-7×MON-00810-6×SYN-IR604-5, NA],A159:[corn, 59122×MON810×NK603, DAS-59122-7×MON-00810-6×MON-00603-6,NA], A160:[corn, 59122×MON810×NK603×MIR604,DAS-59122-7×MON-00810-6×MON-00603-6×SYN-IR604-5, NA], A161: [corn,59122×MON88017, DAS-59122-7×MON-88017-3, NA], A162:[corn,59122×MON88017×DAS40278, DAS-59122-7×MON-88017-3×DAS-40278-9, NA],A163:[corn, 59122×NK603, DAS-59122-7×MON-00603-6,Herculex(trademark)RWRoundupReady(trademark)2], A164:[corn,59122×NK603×MIR604, DAS-59122-7×MON-00603-6×SYN-IR604-5, NA],A165:[corn, 59122×TC1507×GA21, DAS-59122-7×DAS-01507-1×MON-00021-9, NA],A166:[corn, 676, PH-000676-7, NA], A167:[corn, 678, PH-000678-9, NA],A168:[corn, 680, PH-000680-2, NA], A169:[corn, 98140, DP-098140-6,Optimum(trademark)GAT(trademark)], A170:[corn, 98140×59122,DP-098140-6×DAS-59122-7, NA], A171:[corn, 98140×TC1507,DP-098140-6×DAS-01507-1, NA], A172:[corn, 98140×TC1507×59122,DP-098140-6×DAS-01507-1×DAS-59122-7, NA], A173:[corn, Bt10, NA, Bt10],A174:[corn, Bt11(X4334CBR,X4734CBR), SYN-BT011-1,AcTrisure(trademark)CB/LL], A175:[corn, Bt11×5307,SYN-BT011-1×SYN-05307-1, NA], A176:[corn, Bt11×5307×GA21,SYN-BT011-1×SYN-05307-1×MON-00021-9, NA], A177:[corn, Bt11×59122,SYN-BT011-1×DAS-59122-7, NA], A178: [corn, Bt11×59122×GA21,SYN-BT011-1×DAS-59122-7×MON-00021-9, NA], A179: [corn,Bt11×59122×MIR604, SYN-BT011-1×DAS-59122-7×SYN-IR604-5, NA], A180:[corn, Bt11×59122×MIR604×GA21,SYN-BT011-1×DAS-59122-7×SYN-IR604-5×MON-00021-9, NA], A181: [corn,Bt11×59122×MIR604×TC1507,SYN-BT011-1×DAS-59122-7×SYN-IR604-5×DAS-01507-1, NA], A182:[corn,BT11×59122×MIR604×TC1507×GA21,SYN-BT011-1×DAS-59122-7×SYN-IR604-5×DAS-01507-1×MON-00021-9,Agrisure(registered trademark)3122], A183:[corn, Bt11×59122×TC1507,SYN-BT011-1×DAS-59122-7×DAS-01507-1, NA], A184: [corn,Bt11×59122×TC1507×GA21, SYN-BT011-1×DAS-59122-7×DAS-01507-1×MON-00021-9,NA], A185:[corn, Bt11×GA21, SYN-BT011-1×MON-00021-9, Agrisure(trademark) GT/CB/LL] A186: [corn, Bt11×MIR162, SYN-BT011-1×SYN-IR162-4,Agrisure(registered trademark)Viptera(trademark)2100], A187:[corn,Bt11×MIR162×5307, SYN-BT011-1×SYN-IR162-4×SYN-05307-1, NA], A188:[corn,Bt11×MIR162×5307×GA21, SYN-BT011-1×SYN-IR162-4×SYN-05307-1×MON-00021-9,NA], A189: [corn, Bt11×MIR162×GA21, SYN-BT011-1×SYN-IR162-4×MON-00021-9,Agrisure(registered trademark)Viptera(trademark)3110], A190:[corn,BT11×MIR162×MIR604, SYN-BT011-1×SYN-IR162-4×SYN-IR604-5,Agrisure(registered trademark)Viptera(trademark)3100], A191:[corn,BT11×MIR162×MIR604×5307,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×SYN-05307-1, NA], A192: [corn,Bt11×MIR162×MIR604×5307×GA21,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×SYN-05307-1×MON-00021-9, NA], A193:[corn, Bt11×MIR162×MIR604×GA21,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×MON-00021-9, Agrisure(registeredtrademark)Viptera(trademark)3111,Agrisure(registeredtrademark)Viptera(trademark)4], A194:[corn,Bt11×MIR162×MIR604×MON89034×5307×GA21,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×MON-89034-3×SYN-05307-1×MON-00021-9,NA], A195:[corn, BT11×MIR162×MIR604×TC1507,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×DAS-01507-1, NA], A196:[corn,BT11×MIR162×MIR604×TC1507×5307,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×DAS-01507-1×SYN-05307-1, NA],A197:[corn, Bt11×MIR162×MIR604×TC1507×GA21,SYN-BT011-1×SYN-IR162-4×SYN-IR604-5×DAS-01507-1×MON-00021-9, NA],A198:[corn, Bt11×MIR162×MON89034, SYN-BT011-1×SYN-IR162-4×MON-89034-3,NA], A199:[corn, Bt11×MIR162×MON89034×GA21,SYN-BT011-1×SYN-1R162-4×MON-89034-3×MON-00021-9, NA], A200:[corn,Bt11×MIR162×TC1507, SYN-BT011-1×SYN-IR162-4×DAS-01507-1, NA],A201:[corn, Bt11×MIR162×TC1507×5307,SYN-BT011-1×SYN-IR162-4×DAS-01507-1×SYN-05307-1, NA], A202: [corn,Bt11×MIR162×TC1507×5307×GA21,SYN-BT011-1×SYN-IR162-4×DAS-01507-1×SYN-05307-1×MON-00021-9, NA], A203:[corn, Bt11×MIR162×TC1507×GA21,SYN-BT011-1×SYN-IR162-4×DAS-01507-1×MON-00021-9,Agrisure(trademark)Viptera3220], A204:[corn, Bt11×MIR604,SYN-BT011-1×SYN-IR604-5, Agrisure(trademark)CB/LL/RW], A205:[corn,Bt11×MIR604×5307, SYN-BT011-1×SYN-IR604-5×SYN-05307-1, NA], A206:[corn,Bt11×MIR604×5307×GA21, SYN-BT011-1×SYN-IR604-5×SYN-05307-1×MON-00021-9,NA], A207:[corn, BT11×MIR604×GA21, SYN-BT011-1×SYN-IR604-5×MON-00021-9,Agrisure(trademark)3000GT], A208:[corn, Bt11×MIR604×TC1507,SYN-BT011-1×SYN-IR604-5×DAS-01507-1, NA], A209:[corn,Bt11×MIR604×TC1507×5307,SYN-BT011-1×SYN-IR604-5×DAS-01507-1×SYN-05307-1, NA], A210:[corn,Bt11×MIR604×TC1507×GA21,SYN-BT011-1×SYN-IR604-5×DAS-01507-1×MON-00021-9, NA], A211:[corn,Bt11×MON89034, SYN-BT011-1×MON-89034-3, NA], A212:[corn,Bt11×MON89034×GA21, SYN-BT011-1×MON-89034-3×MON-00021-9, NA],A213:[corn, Bt11×TC1507, SYN-BT011-1×DAS-01507-1, NA], A214:[corn,Bt11×TC1507×5307, SYN-BT011-1×DAS-01507-1×SYN-05307-1, NA], A215:[corn,Bt11×TC1507×5307×GA21, SYN-BT011-1×DAS-01507-1×SYN-05307-1×MON-00021-9,NA], A216:[corn, Bt11×TC1507×GA21, SYN-BT011-1×DAS-01507-1×MON-00021-9,NA], A217:[corn, Bt176(176), SYN-EV176-9,NaturGardKnockOut(trademark,Maximizer(trademark)], A218:[corn,BVLA430101, NA, NA], A219:[corn, CBH-351, ACS-ZMO04-3,Starlink(trademark)Maize], A220:[corn, DAS40278, DAS-40278-9,Enlist(trademark)Maize], A221:[corn, DAS40278×NK603,DAS-40278-9×MON-00603-6, NA], A222:[corn, DBT418, DKB-89614-9,BtXtra(trademark)Maize], A223:[corn, DLL25(B16), DKB-89790-5, NA],A224:[corn, GA21, MON-00021-9,RoundupReady(trademark)Maize,Agrisure(trademark)GT], A225:[corn,GA21×MON810, MON-00021-9×MON-00810-6,RoundupReady(trademark)YieldGard(trademark)maize], A226:[corn, GA21×T25,MON-00021-9×ACS-ZMO03-2, NA], A227:[corn, HCEM485, HCEM485, NA],A228:[corn, LY038, REN-00038-3, Mavera(trademark)Maize], A229:[corn,LY038×MON810, REN-00038-3×MON-00810-6,Mavera(trademark)YieldGard(trademark)Maize], A230:[corn, MIR162,SYN-IR162-4, Agrisure(trademark)Viptera], A231:[corn, MIR162×5307,SYN-IR162-4×SYN-05307-1, NA], A232:[corn, MIR162×5307×GA21,SYN-IR162-4×SYN-05307-1×MON-00021-9, NA], A233:[corn, MIR162×GA21,SYN-IR162-4×MON-00021-9, NA], A234:[corn, MIR162×MIR604,SYN-IR162-4×SYN-IR604-5, NA], A235:[corn, MIR162×MIR604×5307,SYN-IR162-4×SYN-IR604-5×SYN-05307-1, NA], A236:[corn,MIR162×MIR604×5307×GA21,SYN-1R162-4×SYN-IR604-5×SYN-05307-1×MON-00021-9, NA], A237:[corn,MIR162×MIR604×GA21, SYN-IR162-4×SYN-IR604-5×MON-00021-9, NA],A238:[corn, MIR162×MIR604×TC1507×5307,SYN-IR162-4×SYN-IR604-5×DAS-01507-1×SYN-05307-1, NA], A239:[corn,MIR162×MIR604×TC1507×5307×GA21,SYN-IR162-4×SYN-1R604-5×DAS-01507-1×SYN-05307-1×MON-00021-9, NA],A240:[corn, MIR162×MIR604×TC1507×GA21,SYN-IR162-4×SYN-IR604-5×DAS-01507-1×MON-00021-9, NA], A241:[corn,MIR162×MON89034, SYN-IR162-4×MON-89034-3, NA], A242:[corn,MIR162×MON89034×GA21, SYN-IR162-4×MON-89034-3×MON-00021-9, NA],A243:[corn, MIR162×NK603, SYN-IR162-4×MON-00603-6, NA], A244:[corn,MIR162×TC1507, SYN-IR162-4×DAS-01507-1, NA], A245:[corn,MIR162×TC1507×5307, SYN-IR162-4×DAS-01207-1×SYN-05307-1, NA],A246:[corn, MIR162×TC1507×5307×GA21,SYN-IR162-4×DAS-01507-1×SYN-05307-1×MON-00021-9, NA], A247:[corn,MIR162×TC1507×GA21, SYN-IR162-4×DAS-01507-1×MON-00021-9, NA],A248:[corn, MIR604, SYN-IR604-5, Agrisure(trademark)RW], A249:[corn,MIR604×5307, SYN-IR604-5×SYN-05307-1, NA], A250:[corn, MIR604×5307×GA21,SYN-IR604-5×SYN-05307-1×MON-00021-9, NA], A251:[corn, MIR604×GA21,SYN-IR604-5×MON-00021-9, Agrisure(trademark)GT/RW], A252:[corn,MIR604×NK603, SYN-IR604-5×MON-00603-6, NA], A253:[corn, MIR604×TC1507,SYN-IR604-5×DAS-01507-1, Optimum(registeredtrademark)TRIsect(trademark)], A254:[corn, MIR604×TC1507×5307,SYN-IR604-5×DAS-01507-1×SYN-05307-1, NA], A255:[corn,M1R604×TC1507×5307×GA21, SYN-IR604-5×TC1507×SYN-05307-1×MON-00021-9,NA], A256:[corn, MIR604×TC1507×GA21, SYN-IR604-5×TC1507×MON-00021-9,NA], A257:[corn, MON801(MON80100), MON801, NA], A258:[corn, MON802,MON-80200-7, NA], A259:[corn, MON809, PH-MON-809-2, NA], A260:[corn,MON810, MON-00810-6, YieldGard(trademark), MaizeGard(trademark)],A261:[corn, MON810×MIR162, MON-00810-6×SYN-IR162-4, NA], A262:[corn,MON810×MIR162×NK603, MON-00810-6×SYN-IR162-4×MON-00603-6, NA], A263:[corn, MON810×MIR604, MON-00810-6×SYN-IR604-5, NA], A264:[corn,MON810×MON88017, MON-00810-6×MON-88017-3, YieldGard(trademark)VTTriple],A265:[corn, MON810×NK603×MIR604, MON-00810-6×MON-00603-6×SYN-IR604-5,NA], A266:[corn, MON832, NA, RoundupReady(trademark)Maize], A267:[corn,MON863, MON-00863-5, YieldGard(trademark)RootwormRW,MaxGard(trademark)],A268:[corn, MON863×MON810, MON-00863-5×MON-00810-6,YieldGard(trademark)Plus], A269:[corn, MON863×MON810×NK603,MON-00603-6×MON-00810-6×MON-00863-5, YieldGard(trademark)PluswithRR],A270:[corn, MON863×NK603, MON-00863-5×MON-00603-6,YieldGard(trademark)RW+RR], A271:[corn, MON87403, MON87403-1, NA],A272:[corn, MON87411, MON-87411-9, NA], A273:[corn, MON87419,MON87419-8, NA], A274:[corn, MON87427, MON-87427-7,RoundupReady(trademark)Maize], A275:[corn, MON87427×59122,MON-87427-7×DAS-59122-7, NA], A276:[corn, MON87427×MON88017,MON-87427-7×MON-88017-3, NA], A277:[corn, MON87427×MON88017×59122,MON-87427-7×MON-88017-3×DAS-59122-7, NA], A278:[corn, MON87427×MON89034,MON-87427-7×MON-89034-3, NA], A279:[corn, MON87427×MON89034×59122,MON-87427-7×MON-89034-3×DAS-59122-7, NA], A280:[corn,MON87427×MON89034×MON88017, MON-87427-7×MON-89034-3×MON-88017-3, NA],A281:[corn, MON87427×MON89034×MON88017×59122,MON-87427-7×MON-89034-3×MON-88017-3×59122, NA], A282:[corn,MON87427×MON89034×NK603, MON-87427-7×MON-89034-3×MON-00603-6, NA],A283:[corn, MON87427×MON89034×TC1507,MON-87427-7×MON-89034-3×DAS-01507-1, NA], A284:[corn,MON87427×MON89034×TC1507×59122,MON-87427-7×MON-89034-3×DAS-01507-1×DAS-59122-7, NA], A285:[corn,MON87427×MON89034×TC1507×MON87411×59122,MON-87427-7×MON-89034-3×DAS-01507-1×MON-87411-9×DAS-59122-7, NA],A286:[corn, MON87427×MON89034×TC1507×MON88017,MON-87427-7×MON-89034-3×DAS-01507-1×MON-88017-3, NA], A287:[corn,MON87427×MON89034×MIR162×NK603,MON-87427-7×MON-89034-3×SYN-IR162-4×MON-00603-6, NA], A288: [corn,MON87427×MON89034×TC1507×MON88017×59122,MON-87427-7×MON-89034-3×DAS-01507-1×MON-88017-3×DAS-59122-7, NA],A289:[corn, MON87427×NK603, MON-87427-7×MON-00603-6, NA], A290:[corn,MON87427×TC1507, MON-87427-7×DAS-01507-1, NA], A291:[corn,MON87427×TC1507×59122, MON-87427-7×DAS-01507-1×DAS-59122-7, NA],A292:[corn, MON87427×TC1507×MON88017,MON-87427-7×DAS-01507-1×MON-88017-3, NA], A293:[corn,MON87427×TC1507×MON88017×59122,MON-87427-7×DAS-01507-1×MON-88017-3×DAS-59122-7, NA], A294:[corn,MON87460, MON-87460-4, Genuity(registered trademark)DroughtGard(trademark)], A295:[corn, MON87460×MON88017,MON-87460-4×MON-88017-3, NA], A296:[corn, MON87460×MON89034×MON88017,MON-87460-4×MON-89034-3×MON-88017-3, NA], A297:[corn,MON87460×MON89034×NK603, MON-87460-4×MON-89034-3×MON-00603-6, NA], A298:[corn, MON87460×NK603, MON-87460-4×MON-00603-6, NA], A299:[corn,MON88017, MON-88017-3,YieldGard(trademark)VT(trademark)Rootworm(trademark)RR2], A300:[corn,MON88017×DAS40278, MON-88017-3×DAS-40278-9, NA], A301:[corn, MON89034,MON-89034-3, YieldGard(trademark)VTPro(trademark)], A302:[corn,MON89034×59122, MON-89034-3×DAS-59122-7, NA], A303:[corn,MON89034×59122×DAS40278, MON-89034-3×DAS-59122-7×DAS-40278-9, NA],A304:[corn, MON89034×59122×MON88017,MON-89034-3×DAS-59122-7×MON-88017-3, NA], A305:[corn,MON89034×59122×MON88017×DAS40278,MON-89034-3×DAS-59122-7×MON-88017-3×DAS-40278-9, NA], A306:[corn,MON89034×DAS40278, MON-89034-3×DAS-40278-9, NA], A307:[corn,MON89034×MON87460, MON-89034-3×MON-87460-4, NA], A308:[corn,MON89034×MON88017, MON-89034-3×MON-88017-3, Genuity(registeredtrademark)VTTriplePro(trademark)], A309:[corn,MON89034×MON88017×DAS40278, MON-89034-3×MON-88017-3×DAS-40278-9, NA],A310:[corn, MON89034×NK603, MON-89034-3×MON-00603-6, Genuity(registeredtrademark)VTDoublePro(trademark)], A311:[corn, MON89034×NK603×DAS40278,MON-89034-3×MON-00603-6×DAS-40278-9, NA], A312:[corn, MON89034×TC1507,MON-89034-3×DAS-01507-1, NA], A313:[corn, MON89034×TC1507×59122,MON-89034-3×DAS-01507-1×DAS-59122-7, NA], A314:[corn,MON89034×TC1507×59122×DAS40278,MON-89034-3×DAS-01507-1×DAS-59122-7×DAS-40278-9, NA], A315:[corn,MON89034×TC1507×DAS40278, MON-89034-3×DAS-01507-1×DAS-40278-9, NA],A316:[corn, MON89034×TC1507×MON88017,MON-89034-3×DAS-01507-1×MON-88017-3, NA], A317:[corn,MON89034×TC1507×MON88017×59122,MON-89034-3×DAS-01507-1×MON-88017-3×DAS-59122-7, Genuity(registeredtrademark) SmartStax(trademark)], A318:[corn,MON89034×TC1507×MON88017×59122×DAS40278,MON-89034-3×1DAS-01507-1×MON-88017-3×DAS-59122-7×DAS-40278-9, NA],A319:[corn, MON89034×TC1507×MON88017×DAS40278,MON-89034-3×DAS-01507-1×MON-88017-3×DAS-59122-7×DAS-40278-9, NA],A320:[corn, MON89034×TC1507×NK603, MON-89034-3×DAS-01507-1×MON-00603-6,PowerCore(trademark)], A321:[corn, MON89034×TC1507×NK603×DAS40278,MON-89034-3×DAS-01507-1×MON-00603-6×DAS-40278-9, NA], A322:[corn,MON89034×TC1507×NK603×M1R162,MON-89034-3×DAS-01507-1×MON-00603-6×SYN-IR162-4, NA], A323:[corn,MON89034×TC1507×NK603×MIR162×DAS40278,MON-89034-3×1DAS-01507-1×MON-00603-6×SYN-1R162-4×DAS-40278-9, NA],A324:[corn, MON89034×GA21, MON-89034-3×MON-00021-9, NA], A325:[corn,MS3, ACS-ZM001-9, InVigor(trademark)Maize], A326:[corn, MS6,ACS-ZM005-4, InVigor(trademark)Maize], A327:[corn, MZHGOJG, SYN-000JG-2,NA], A328:[corn, MZIR098, SYN-00098-3, NA], A329:[corn, NK603,MON-00603-6, RoundupReady(trademark)2Maize], A330:[corn,NK603×MON810×4114×M1R604,MON-00603-6×MON-00810-6×DP004114-3×SYN-1R604-4, NA], A331:[corn,NK603×MON810, MON-00603-6×MON-00810-6, YieldGard(trademark)CB+RR],A332:[corn, NK603×T25, MON-00603-6×ACS-ZMO03-2,RoundupReady(trademark)LibertyLink(trademark)Maize], A333:[corn, T14,ACS-ZM002-1, LibertyLink(trademark)Maize], A334:[corn, T25, ACS-ZM003-2,LibertyLink(trademark)Maize], A335:[corn, T25×MON810,ACS-ZMO03-2×MON-00810-6,LibertyLink(trademark)Yieldgard(trademark)Maize], A336:[corn, TC1507,DAS-01507-1, Herculex(trademark)I,Herculex(trademark)CB], A337: [corn,TC1507×59122×MON810×MIR604×NK603,DAS-01507-1×DAS-59122-7×MON-00810-6×SYN-IR604-5×MON-00603-6,Optimum(trademark)IntrasectXtreme], A338:[corn,TC1507×MON810×MIR604×NK603,DAS-01507-1×MON-00810-6×SYN-IR604-5×MON-00603-6, NA], A339: [corn,TC1507×5307, DAS-01507-1×SYN-05307-1, NA], A340: [corn,TC1507×5307×GA21, DAS-01507-1×SYN-05307-1×MON-00021-9, NA], A341:[corn,TC1507×59122, DAS-01507-1×DAS-59122-7, Hercule×XTRA(trademark)],A342:[corn, TC1507×59122×DAS40278, DAS-01507-1×DAS-59122-7×DAS-40278-9,NA], A343:[corn, TC1507×59122×MON810,DAS-01507-1×DAS-59122-7×MON-00810-6, Optimum(registeredtrademark)AcreMax(registered trademark)Xtra], A344:[corn,TC1507×59122×MON810×MIR604,DAS-01507-1×DAS-59122-7×MON-00810-6×SYN-IR604-5, Optimum(registeredtrademark) AcreMax(registered trademark) XTreme], A345:[corn,TC1507×59122×MON810×NK603,DAS-01507-1×DAS-59122-7×MON-00810-6×MON-00603-6,Optimum(trademark)IntrasectXTRA], A346:[corn, TC1507×59122×MON88017,DAS-01507-1×DAS-59122-7×MON-88017-3, NA], A347:[corn,TC1507×59122×MON88017×DAS40278,DAS-01507-1×DAS-59122-7×MON-88017-3×DAS-40278-9, NA], A348:[corn,TC1507×59122×NK603, DAS-01507-1×DAS-59122-7×MON-00603-6,Hercule×XTRA(trademark)RR], A349:[corn, TC1507×59122×NK603×MIR604,DAS-01507-1×DAS-59122-7×MON-00603-6×SYN-IR604-5, NA], A350:[corn,TC1507×DAS40278, DAS-01507-1×DAS-40278-9, NA], A351:[corn, TC1507×GA21,DAS-01507-1×MON-00021-9, NA], A352:[corn, TC1507×MIR162×NK603,DAS-01507-1×SYN-IR162-4×MON-00603-6, Optimum(registeredtrademark)Leptra(trademark)], A353:[corn, TC1507×MIR604×NK603,DAS-01507-1×SYN-IR604-5×MON-00603-6, Optimum(trademark)TRIsect],A354:[corn, TC1507×MON810, DAS-01507-1×MON-00, NA], A355:[corn,TC1507×MON810×MIR162, DAS-01507-1×MON-00810-6×SYN-IR162-4, NA], A356:[corn, TC1507×MON810×MIR162×NK603,DAS-01507-1×MON-00810-6×SYN-IR162-4×MON-00603-6, NA], A357:[corn,TC1507×MON810×MIR604, DAS-01507-1×MON-00810-6×SYN-IR604-5, NA],A358:[corn, TC1507×MON810×NK603, DAS-01507-1×MON-00810-6×MON-00603-6,Optimum(trademark)Intrasect], A359:[corn, TC1507×MON810×NK603×MIR604,DAS-01507-1×MON-00810-6×MON-00603-6×SYN-IR604-5, NA], A360:[corn,TC1507×MON88017, DAS-01507-1×MON-88017-3, NA], A361: [corn,TC1507×MON88017×DAS40278, DAS-01507-1×MON-88017-3×DAS-40278-9, NA],A362:[corn, TC1507×NK603, DAS-01507-1×MON-00603-6,Herculex(trademark)IRR], A363:[corn, TC1507×NK603×DAS40278,DAS-01507-1×MON-00603-6×DAS-40278-9, NA], A364:[corn, TC6275,DAS-06275-8, NA], A365:[corn, VCO-01981-5, VCO-01981-5, NA], A366:[corn,DK404SR, DK404SR, NA], A367:[corn, EXP1910IT, EXP1910IT, NA],A368:[melon, MelonA, NA, NA], A369:[melon, MelonB, NA, NA],A370:[papaya, 55-1, CUH-CP551-8, Rainbow,SunUp], A371:[papaya, 63-1,CUH-CP631-7, NA], A372:[papaya, HuanongNo.1, NA, HuanongNo.1],A373:[papaya, X17-2, UFL-X17CP-6, NA], A374:[petunia, Petunia-CHS, NA,NA], A375:[plum, C-5, ARS-PLMC5-6, NA], A376:[Polish canola, HCR-1, NA,NA], A377:[Polish canola, ZSR500, NA,Hysyn101RRRoundup-Ready(trademark)], A378:[Polish canola, ZSR502, NA,Hysyn101RRRoundup-Ready(trademark)], A379:[Polish canola, ZSR503, NA,Hysyn101RRRoundup-Ready(trademark)], A380:[poplar,Btpoplar,poplar12(Populusnigra), NA, NA], A381:[poplar,Hybridpoplarclone741, NA, NA], A382:[potato, 1210amk, NA,Lugovskoiplus], A383:[potato, 2904/lkgs, NA, Elizavetaplus],A384:[potato, AM04-1020, BPS-A1020-5, StarchPotato], A385:[potato,ATBT04-27, NMK-89367-8, AtlanticNewLeaf(trademark)potato], A386:[potato,ATBT04-30, NMK-89613-2, AtlanticNewLeaf(trademark)potato], A387:[potato,ATBT04-31, NMK-89170-9, AtlanticNewLeaf(trademark)potato], A388:[potato,ATBT04-36, NMK-89279-1, AtlanticNewLeaf(trademark)potato], A389:[potato,ATBT04-6, NMK-89761-6, AtlanticNewLeaf(trademark)potato], A390:[potato,BT06, NMK-89812-3, NewLeaf(trademark)RussetBurbankpotato], A391:[potato,BT10, NMK-89175-5, NewLeaf(trademark)RussetBurbankpotato], A392:[potato,BT12, NMK-89601-8, NewLeaf(trademark)RussetBurbankpotato], A393:[potato,BT16, NMK-89167-6, NewLeaf(trademark)RussetBurbankpotato], A394:[potato,BT17, NMK-89593-9, NewLeaf(trademark)RussetBurbankpotato], A395:[potato,BT18, NMK-89906-7, NewLeaf(trademark)RussetBurbankpotato], A396:[potato,BT23, NMK-89675-1, NewLeaf(trademark)RussetBurbankpotato], A397:[potato,E12, SPS-00E12-8, Innate(registered trademark)Cultivate], A398:[potato,E24, SPS-00E24-2, NA], A399:[potato, EH92-527-1, BPS-25271EH92-527-1-9,Amflora(trademark)], A400:[potato, F10, SPS-00F10-7, Innate(registeredtrademark)Generate], A401:[potato, F37, SPS-00F37-7, NA], A402:[potato,G11, SPS-00G11-9, NA], A403:[potato, H37, SPS-00H37-9, NA],A404:[potato, H50, SPS-00H50-4, NA], A405:[potato, HLMT15-15, NA,Hi-LiteNewLeaf(trademark)Ypotato], A406:[potato, HLMT15-3, NA,Hi-LiteNewLeaf(trademark)Ypotato], A407:[potato, HLMT15-46, NA,Hi-LiteNewLeaf(trademark)Ypotato], A408:[potato, J3, SPS-000J3-4,Innate(registered trademark)Accelerate], A409:[potato, J55, SPS-00J55-2,NA], A410:[potato, J78, SPS-00J78-7, NA], A411:[potato, RBMT15-101,NMK-89653-6, NewLeaf(trademark)YRussetBurbankpotato], A412:[potato,RBMT21-129, NMK-89684-1, NewLeaf(trademark)PlusRussetBurbankpotato],A413:[potato, RBMT21-152, NA,NewLeaf(trademark)PlusRussetBurbankpotato], A414:[potato, RBMT21-350,NMK-89185-6, NewLeaf(trademark)PlusRussetBurbankpotato], A415:[potato,RBMT22-082, NMK-89896-6, NewLeaf(trademark)PlusRussetBurbankpotato],A416:[potato, RBMT22-186, NA,NewLeaf(trademark)PlusRussetBurbankpotato], A417:[potato, RBMT22-238,NA, NewLeaf(trademark)PlusRussetBurbankpotato], A418:[potato,RBMT22-262, NA, NewLeaf(trademark)PlusRussetBurbankpotato],A419:[potato, SEMT15-02, NMK-89935-9, ShepodyNewLeaf(trademark)Ypotato],A420:[potato, SEMT15-07, NA, ShepodyNewLeaf(trademark)Ypotato],A421:[potato, SEMT15-15, NMK-89930-4, ShepodyNewLeaf(trademark)Ypotato],A422:[potato, SPBT02-5, NMK-89576-1, SuperiorNewLeaf(trademark)potato],A423:[potato, SPBT02-7, NMK-89724-5, SuperiorNewLeaf(trademark)potato],A424:[potato, TIC-AR233-5, TIC-AR233-5, NA], A425:[potato, V11,SPS-00V11-6, Innate(registered trademark) Invigorate], A426:[potato, W8,SPS-000W8-4, Innate(registered trademark)Glaciate], A427:[potato, X17,SPS-00X17-5, Innate(registered trademark)Acclimate], A428:[potato, Y9,SPS-000Y9-7, Innate(registered trademark)Hibernate], A429:[rice,7Crp#10, NA, NA], A430:[rice, GMShanyou63, NA, BTShanyou63], A431:[rice,Huahui-1/TT51-1, NA, Huahui-1], A432:[rice, LLRICF06, ACS-0S001-4,LibertyLink(trademark)rice], A433:[rice, LLRICE601, BCS-OS003-7,LibertyLink(trademark)rice], A434:[rice, LLRICE62, ACS-0S002-5,LibertyLink(trademark)rice], A435:[rice, Tarommolaii+crylAb, NA, NA],A436:[rice, CL121×CL141×CFX51, CL121×CL141×CFX51,Clearfield(trademark)Rice], A437:[rice, IMINTA-1×IMINTA-4,IMINTA-1×IMINTA-4, Clearfield(trademark)Rice], A438:[rice, PWC16, PWC16,NA], A439:[rose, WKS82/130-4-1, IFD-52401-4, NA], A440:[rose,WKS92/130-9-1, IFD-52901-9, NA], A441:[soybean,260-05(G94-1,G94-19,G168), DD-026005-3, NA], A442:[soybean, A2704-12,ACS-GM005-3, LibertyLink(trademark)soybean], A443:[soybean, A2704-21,ACS-GM004-2, LibertyLink(trademark)soybean], A444:[soybean, A5547-127,ACS-GM006-4, LibertyLink(trademark)soybean], A445:[soybean, A5547-35,ACS-GM008-6, LibertyLink(trademark)soybean], A446:[soybean, CV127,BPS-CV127-9, Cultivance], A447:[soybean, DAS44406-6, DAS-44406-6, NA],A448:[soybean, DAS68416-4, DAS-68416-4, Enlist(trademark)Soybean],A449:[soybean, DAS68416-4×MON89788, DAS-68416-4×MON-89788-1, NA],A450:[soybean, DAS81419, DAS-81419-2, NA], A451:[soybean,DAS81419×DAS44406-6, DAS-81419-2×DAS-44406-6, NA], A452:[soybean,DP305423, DP-305423-1, Treus(trademark) or, Plenish(trademark)],A453:[soybean, DP305423×GTS40-3-2, DP-305423-1×MON-04032-6, NA],A454:[soybean, DP356043, DP-356043-5, OptimumGAT(trademark)],A455:[soybean, FG72(FG072-2,FG072-3), MST-FG072-3, NA], A456:[soybean,FG72×A5547-127, MST-FG072-3×ACS-GM006-4, NA], A457:[soybean,GTS40-3-2(40-3-2), MON-04032-6, RoundupReady(trademark)soybean],A458:[soybean, GU262, ACS-GM003-1, LibertyLink(trademark)soybean],A459:[soybean, IND-00410-5, IND-00410-5, erdecaHB4Soybean],A460:[soybean, MON87701, MON-87701-2, NA], A461:[soybean,MON87701×MON89788, MON-87701-2×MON-89788-1,Intacta(trademark)RoundupReady(trademark)2Pro], A462:[soybean, MON87705,MON-87705-6, VistiveGold(trademark)], A463:[soybean, MON87705×MON87708,MON-87705-6×MON-87708-9, NA], A461:[soybean, MON87705×MON87708×MON89788,MON-87705-6×MON-87708-9×MON-89788-1, NA], A465:[soybean,MON87705×MON89788, MON-87705-6×MON-89788-1, NA], A466:[soybean,MON87708, MON-87708-9, Genuity(registeredtrademark)RoundupReady(trademark)2 Xtend(trademark)], A167:[soybean,MON87708×MON89788, MON-87708-9×MON-89788-1, Roundup Ready 2Xtend(registered trademark)NA], A468:[soybean, MON87712, MON-87712-4,NA], A469:[soybean, MON87751, MON-87751-7, NA], A470:[soybean,MON87751×MON87701×MON87708×MON89788,MON-87751-7×MON-87701-2×MON87708×MON89788, NA], A171:[seybean, MON87769,MON87769-7, NA], A472:[soybean, MON87769×MON89788,MON-87769-7×MON-89788-1, NA], A473:[soybean, MON89788, MON-89788-1,Genuity(registered trademark)Roundup Ready 2 Yield(trademark)],A474:[soybean, SYHTOH2, SYN-000H2-5, Herbicide-tolerantSoybeanline],A475:[soybean, W62, ACS-GM002-9, LibertyLink(trademark)soybean],A476:[soybean, W98, ACS-GM001-8, LibertyLink(trademark)soybean],A477:[soybean, OT96-15, OT96-15, NA], A478:[Cucurbitapepo, CZW3,SEM-OCZW3-2, NA], A479:[Cucurbita pepo, ZW20, SEM-0ZW20-7, NA],A480:[sugar beet, GTSB77(T9100152), SY-GTSB77-8,InVigor(trademark)sugarbeet], A481:[sugar beet, H7-1, KM-000H71-4,Roundup Ready(trademark)sugarbeet], A482:[sugar beet, T120-7,ACS-BV001-3, LibertyLink(trademark)sugarbeet], A483:[sugarcane,CTB141175/01-A, CTB141175/01-A, NA], A484:[sugarcane, NXI-1T, NXI-1T,NA], A485:[sugarcane, NXI-4T, NXI-4T, NA], A486:[sugarcane, NXI-6T,NXI-6T, NA], A487:[sunflower, X81359, X81359,Clearfield(trademark)Sunflower], A488:[sweet pepper, PK-SP01, X81359,NA], A489:[tobacco, C/F/93/08-02, NA, NA], A490:[tobacco, Vector21-41,NA, NA], A491:[tomato, 1345-4, NA, NA], A492:[tomato, 35-1-N, NA, NA],A493:[tomato, 5345, NA, NA], A494:[tomato, 8338, CGN-89322-3, NA],A495:[tomato, B, SYN-0000B-6, NA], A496:[tomato, Da, SYN-000DA-9, NA],A497:[tomato, DaDongNo9, NA, NA], A498:[tomato,F(1401F,h38F,11013F,7913F), SYN-0000E-1, FLAVRSAVR(trademark)],A499:[tomato, FLAVRSAVR, CGN-89564-2, FLAVRSAVR(trademark)],A500:[tomato, HuafanNol, NA, NA], A501:[tomato, PK-TM8805R(8805R), NA,NA], A502:[wheat, MON71800, MON-71800-3, RoundupReady(trademark)wheat],A503: [wheat, AP205CL, AP205CL, Clearfield(trademark)Wheat],A504:[wheat, AP602CL, AP602CL, Clearfield(trademark)Wheat], A505:[wheat,BW255-2×BW238-3, BW255-2×BW238-3, Clearfield(trademark)Wheat],A506:[wheat, BW7, BW7, Clearfield(trademark)Wheat], A507:[wheat,Teal11A, Tea111A, Clearfield(trademark)Wheat], A508:[wheat, SWP965001,SWP965001, NA], A509:[corn, NA, NA, Agrisure Artesian(trademark)], A510:[corn, NA, NA, Optimum(registered trademark)AQUAmax(registeredtrademark)], A511:[canola, NA, NA, SUcanola(registered trademark)], A512:[canola, NA, NA, Nexera(registeredtrademark)Canola], A513:[soybean, NA, NA, STS soybean], A514:[corn, NA,NA, SR corn], A514:[sunflower, NA, NA, ExpressSun(registered trademark)], A515:[rice, NA, NA, Golden rice], A516:[corn, NA, NA, PoastProtected(registered trademark) corn], A517:[sunflower, NA, NA,ExpressSun(registered trademark)], A518:[rice, NA, NA,Rrovisia(trademark)Rice], A519:[canola, NA, NA, Triazinon TolerantCanola], A520:[corn, NA, NA, SmartStax(registered trademark) Pro],A521:[soybean, NA, NA, Credenz(registered trademark)soybean],A522:[corn, TC1507×59122, DAS-01507-1×DAS-59122-7, Optimum(registeredtrademark)AcreMax(registered trademark)1], A523:[corn,4114×MON810×MIR604, DP-004114-3×MON-00810-6×SYN-IR604-5,Qrome(trademark)], A524:[cotton, NA, NA, Stoneville(registeredtrademark) Cotton ST5517GLTP], A525:[cotton, NA, NA,Stoneville(registeredtrademark)Cotton ST4848GLT], A526:[cotton, NA, NA,Stoneville(registered trademark)Cotton ST4949GLT], A527:[cotton, NA, NA,Stoneville(registered trademark) Cotton ST5020GLT], A528: [cotton, NA,NA, Stoneville (registered trademark) Cotton ST5115GLT], A529:[cotton,NA, NA, Stoneville(registered trademark) Cotton ST6182GLT],A530:[cotton, NA, NA, Stoneville(registered trademark) CottonST4747GLB2], A531:[cotton, NA, NA, Stoneville(registeredtrademark)CottonST4946GLB2], A532:[cotton, NA, NA, Stoneville(registered trademark)Cotton ST6448GLB2], A533:[soybean, NA, NA, Enlist E3(trademark)],A534:[soybean, NA, NA, Enlist(trademark)Roundup Ready 2 Yield(registeredtrademark)], A535:[corn, NA, NA, Enlist(trademark)RoundupReady(registered trademark)Corn 2], A536:[corn, NA, NA,Smartstax(registered trademark)Enlist(trademark)], A537:[corn, NA, NA,Powercore(registered trademark)Enlist(trademark)], A538:[cotton, NA, NA,Enlist(trademark)Cotton], A539:[cotton, NA, NA, Bollgard 3(registeredtrademark)XtendFlex(registered trademark)], A540:[corn, NA, NA,SmartStax(registered trademark)RIB Complete(registered trademark) Corn],A541:[corn, NA, NA, VT Double PRO(registered trademark)RIBComplete(registered trademark)Corn], A542:[corn, NA, NA, VT DoublePRO(registered trademark) Corn], A543:[corn, NA, NA, Genuity(registeredtrademark)VT Triple PRO(registered trademark) RIB Complete Corn],A544:[corn, NA, NA, Trecepta(trademark) Corn], A545:[soybean, NA, NA,FUKUNOMINORI], A546: [ soybean, NA, NA, Yumeminori], A547:[corn, NA, NA,ZFN-12 maize], A548:[rice, NA, NA, Yumepirika], A549:[rice NA, NA, KantoBPH No.1], A550:[cotton, NA, MON88702, NA]

In the present invention, a neonicotinoide type compound, diamide typecompound, carbamate type compound, organic phosphorous type compound,biological nematicidal compound, other insecticidal compound andnematicidal compound, azole type compound, strobilurin type compound,metalaxyl type compound, SDHI compound, other fungicidal compound, orplant growth regulator may be applied as the compound of a compoundgroup A consisting of an insecticidal compound, nematicidal compound,fungicidal compound, or plant growth regulator in the cultivation ofcrops.

As the neonicotinoid compound which may be applied to crop seeds in thepresent invention, the following compounds are given as examples.clothianidin, imidacloprid, nitenpyram, acetamiprid, thiamethoxam,flupyradifurone, sulfoxaflor, triflumezopyrim, dicloromezotiaz,thiacloprid, and dinotefuran.

As the diamide type compound which may be applied to crop seeds in thepresent invention, the following compounds are given as examples.flubendiamide, chlorantraniliprole, cyantraniliprole, cyclaniliprole,broflanilide, tetraniliprole, and cyhalodiamide.

As the carbamate type compound which may be applied to crop seeds in thepresent invention, the following compounds are given as examples.aldicarb, oxamyl, thiodicarb, carbofuran, carbosulfan, and dimethoate.

As the organic phosphorous type compound which may be applied to cropseeds in the present invention, the following compounds are given asexamples. fenamiphos, imicyafos, fensulfothion, terbufos, fosthiazate,phosphocarb, dichlofenthion, isamidofos, isazophos, ethoprophos,cadusafos, chlorpyrifo, heterofos, mecarphon, phorate, thionazin,triazophos, diamidafos, fosthietan, and phosphamidon.

As the biological nematicide compound which may be applied to crop seedsin the present invention, the following compounds are given as examples.Harpin Protein, Pasteuria nishizawae, Pasteuria penetrans, Pasteuriausage, Myrothecium verrucaria, Burholderia cepacia, Bacilluschitonosporus, Paecilomyces lilacinus, Bacillus amyloliquefaciens,Bacillus firmus, Bacillus subtillis, Bacillus pumulis, Trichodermaharzianum, Hirsutella rhossiliensis, Hirsutella minnesotensis,Verticillium chlamydosporum, and Arthrobotrys dactyloides.

As the other insecticidal compound which may be applied to crop seeds inthe present invention, the following compounds are given as examples.fipronil, ethiprole, beta-cyfluthrin, tefluthrin, chlorpyrifos,abamectin, spirotetramat, tioxazafen, fluazaindolizine, fluensulfone,and fluxametamide.

As the azole type compound which may be applied to crop seeds in thepresent invention, the following compounds are given as examples.azaconazole, bitertanol, bromuconazole, cyproconazole, difenoconazole,diniconazole, epoxyconazole, fenbuconazole, fluquinconazole,flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole,metconazole, myclobutanil, penconazole, propiconazole, prothioconazole,simeconazole, tebuconazole, tetraconazole, triadimenol, triticonazole,fenarimol, nuarimol, pyrifenox, imazalil, oxpoconazole fumarate,efurazoate, prochloraz, triflumizole, ipfentrifluconazole, andmefentrifluconazole.

As the strobilurin type compound which may be applied to crop seeds inthe present invention, the following compounds are given as examples.kresoxim-methyl, azoxystrobin, trifloxystrobin, fluoxastrobin,picoxystrobin, pyraclostrobin, dimoxystrobin, pyribencarb,metominostrobin, orysastrobin, and mandestrobin.

As the metalaxyl type compound which may be applied to crop seeds in thepresent invention, the following compounds are given as examples.Metalaxyl and metalaxyl-M or mefenoxam.

As the SDHI compound which may be applied to crop seeds in the presentinvention, the following compounds are given as examples. sedaxane,penflufen, carboxin, boscalid, furametpyr, flutolanil, fluxapyroxad,isopyrazam, fluopyram, isofetamid, pyraziflumid, pydiflumetofen,fluindapyr, inpyrfluxam, and thifluzamide.

As the plant growth regulator which may be applied to crop seeds in thepresent invention, the following compounds are given as examples.Ethephon, chlormequat-chloride, mepiquat-chloride,4-oxo-4-(2-phenyethyl)aminobutylic acid (hereinafter also referred to as“compound 2”).

As the other fungicidal compounds which may be applied to crop seeds inthe present invention, the following compounds are given as examples.Tolclofos-methyl, thiram, captan, carbendazim, thiophanate-methyl,mancozeb, thiabendazole, isotianil, triazoxide, picarbutrazox, andoxathiapiprolin.

In the present invention, the compounds of the above group A to beapplied to seeds are known compounds and can be synthesized based onalready published technical literatures, and also, commerciallyavailable formulations and standard products can be obtained from themarket to use.

In the present invention, compounds of a group B consisting ofinsecticidal compounds or fungicidal compounds may be applied to plantstems and leaves of a crop in the growing period of the crop.Specifically, examples of the compounds of the group B include astrobilurin type compound, azole type compound, SDHI compound,pyrethroid type compound, benzoylphenylurea compound, organicphosphorous type insecticidal compound, neonicotinoid type compound, anddiamide type compound. [0066]

In the present invention, examples of the strobilurin type compoundapplied for the foliar treatment in the growing period of a crop includethe following compounds: Pylacrostrobin, azoxystrobin, mandestrobin,trifloxystrobin, and picoxystrobin.

In the present invention, examples of the azole type compound appliedfor the foliar treatment in the growing period of a crop include thefollowing compounds: Prothioconazole, epoxiconazole, tebuconazole,cyproconazole, propiconazole, metconazole, bromuconazole, tetraconazole,triticonazole, ipfentrifluconazole, and mefentrifluconazole.

In the present invention, examples of the SDHI compound applied for thefoliar treatment in the growing period of a crop include the followingcompounds: Benzovindiflupyl, bixafen, fluxapyroxad, fluindapyr, andinpyrfluxam.

In the present invention, other examples of the fungicidal compoundapplied for the foliar treatment in the growing period of a crop includethe following compounds: Tolclofos-methyl and ethaboxam.

In the present invention, examples of the pyrethroid type compoundapplied for the foliar treatment in the growing period of a crop includethe following compounds: Bifenthrin, A-cyhalothrin, y-cyhalothrin,cypermethrin, fenpropathrin, ethofenprox, silafluofen, andesfenvalerate.

In the present invention, examples of the benzoylphenylurea compoundapplied for the foliar treatment in the growing period of a crop includethe following compounds: Teflubenzuron and triflumuron.

In the present invention, examples of the organic phosphorous typeinsecticidal compound applied for the foliar treatment in the growingperiod of a crop include the following compounds: Acephate and methomyl.

In the present invention, examples of the neonicotinoid typeinsecticidal compound applied for the foliar treatment in the growingperiod of a crop include the following compounds: Imidacloprid,clothianidin, thiamethoxam, sulfoxaflor, flupyradifurone,triflumezopyrim, and dicloromezotiaz.

In the present invention, examples of the diamide type insecticidalcompound applied for the foliar treatment in the growing period of acrop include the following compounds: Flubendiamide,chlorantraniliprole, cyantraniliprole, broflanilide, tetraniliprol, andcyhalodiamide.

In the present invention, the compounds of the above group B to beapplied to foliar treatment are known compounds and can be synthesizedbased on already known patent literatures, and also, commerciallyavailable formulations and standard products can be obtained from themarket to use.

In the case of applying a compound of the group A to crop seeds, thecompound of the group A is usually mixed with a carrier such as a solidcarrier and liquid carrier and, according to the need, further addedwith a formulation auxiliary agent such as a surfactant to make aformualation. Preferable formualtion type is an aqueous liquidsuspension formulation.

As the compounds of the group A which may be applied to crop seeds inthe present invention, formulations each containing a single ingredientmay be used either singly or in combinations, or a formulationcontaining two or more ingredients may be used.

The amount of the compound of the group A to be used for the treatmentis in a range of generally 0.2 to 5000 g and preferably 0.5 to 1000 gper 100 kg of seeds. Examples of the method of applying activeingredients to plant seeds include a method in which the seed ispowder-coated with a formulation containing the active ingredients, amethod in which the seed is dipped in the formulation containing theactive ingredients, a method in which the formulation containing theactive ingredients is sprayed onto the seed, and a method in which theseed is coated with the carrier containing the active ingredients.

In the present invention, ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetateis applied to a cultivation area before and/or after the crop seeds aresown.

In the present invention, any crop cultivation area may be used withoutany particular limitation as long as it is a place capable of growingcrops and examples of the cultivation area include a farmland,cultivation field, no-till field, park, levee, nursery tray, nurserybox, and nursery land.

Ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,1-dioxo-1,2,3,1-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetateis a known compound and is represented by the formula 1. This compoundis hereinafter called “compound X”. The compound X may be manufacturedby a known method such as U.S. Pat. No. 6,537,948.

In a step of applying saflufenacil, trifludimoxazin, flumioxazin, orcompound X, each of these compounds is usually mixed with a carrier suchas a solid carrier or liquid carrier, to which a formulation auxiliaryagent such as a surfactant is further added according to the need tomake a formulation. The formulation type is preferably an aqueous liquidsuspension, oil suspension, wettable powder, water dispersible granule,granule, aqueous emulsion, oil-based emulsion, and emulsifiableconcentrate, among which the emulsifiable concentrate is morepreferable. Moreover, a formulation containing compound X singly as anactive ingredient may be independently used or may be used incombination with a formulation containing other herbicides as activeingredients. Also, a formulation containing saflufenacil,trifludimoxazin, flumioxazin, or compound X, and other herbicide asactive ingredients may be used. Also, a formulation containingsaflufenacil, trifludimoxazin, flumioxazin, or compound X, and otherherbicide as active ingredients may be used in combination with aformulation containing other herbicide as active ingredients.

Examples of the method of applying saflufenacil, trifludimoxazin,flumioxazin, or compound X onto a cultivation area include a method inwhich each of these compounds is sprayed on the soil of the cultivationarea and a method in which each of these compounds is sprayed on weedsafter these weeds sprout. The spraying is usually performed afterdiluting the compound in water and the amount of water to be sprayed isusually 50 to 1000 L/ha, preferably 100 to 500 L/ha, and more preferably150 to 300 L/ha though no particular limitation is imposed on it.

The amount of saflufenacil, trifludimoxazin, flumioxazin, or compound Xto be used in the step of applying it onto a cultivation area isgenerally 1 to 1000 g, preferably 2 to 500 g, more preferably 5 to 200g, and even more preferably 10 to 100 g per 10000 of the cultivationarea. In the step of applying saflufenacil, trifludimoxazin,flumioxazin, or compound X for treatment in a cultivation area, anadjuvant may be added to saflufenacil, trifludimoxazin, flumioxazin, orcompound X, followed by applying. Although no particular limitation isimposed on the kind of adjuvant, examples of the adjuvant include an oiltype such as Agri-Dex or MSO, nonionic type (ester or ether ofpolyoxyethylene) such as Induce, anionic type (substituted sulfonate)such as gramine S, and cationic type (polyoxyethyleneamine)such asGenamin T 200 BM, and organic silicone type such as Silwett L77.

The pH and hardness of the spraying liquid when applying saflufenacil,trifludimoxazin, flumioxazin, or compound X for treatment in acultivation area are usually in a range from pH 5 to 9 and in a rangefrom 0 to 500 in hardness though no particular limitation is imposed onthe pH and hardness. When applying saflufenacil, trifludimoxazin,flumioxazin, or compound X for treatment in a cultivation area, the timeperiod of the application is usually in a range from 5 a.m. to 9 p.m.,though no particular limitation is imposed on the time period and thephoton flux density is usually 10 to 2500 _(i)imol/m²/s.

In the present invention, crop seeds are sown in a cultivation areausing a usual method. In the method of controlling resistant weedsaccording to the present invention, saflufenacil, trifludimoxazin,flumioxazin, or compound X may be applied for the treatment beforesowing crop seeds or may be applied when and/or after sowing crop seeds.Specifically, the number of treatments using saflufenacil,trifludimoxazin, flumioxazin, or compound X is any one of one timebefore, when, or after sowing crop seeds, two times excluding the timebefore sowing crop seeds, two times excluding the time when sowing cropseeds, or two times excluding the time after sowing crop seeds, or threetimes allotted to perform treatment in every timing before, when, andafter the sowing.

In the case of applying saflufenacil, trifludimoxazin, flumioxazin, orcompound X before sowing crop seeds, the compound is applied usuallybetween 50 days before sowing and just before sowing, preferably between30 days before sowing and just before sowing, more preferably between 20days before sowing and just before sowing, and even more preferablybetween 10 days before sowing and just before sowing.

In the case of applying saflufenacil, trifludimoxazin, flumioxazin, orcompound X after sowing the crop seeds, the compound is usually appliedduring a period just after sowing and before blooming. The compound ismore preferably applied during a period just after sowing and beforesprouting or during a period between 1 and 6 leaf stages of the crop.When applying saflufenacil, trifludimoxazin, flumioxazin, or compound Xto perform foliar treatment during a period between 1 and 6 leaf stagesof the crop, this treatment may be performed simultaneously when orsequentially before or after performing the aforementioned foliartreatment using the compound of the group B. In the case of thesequential treatments, there is no particular limitation to the order.

The case where saflufenacil, trifludimoxazin, flumioxazin, or compound Xis applied when sowing crop seeds implies the case where a seedingmachine is integrated with a spraying machine.

When a compound of the above group B is applied for foliar treatmentduring a crop growing period, the compound of the group B is usuallymixed with a carrier such as a solid carrior or liquid carrier and,according to the need, a formulation auxiliary agent such as asurfactant is added to make a formulation. The formulation is preferablyan emulsifiable concentrate, aqueous suspension, or aqueous solubleliquid.

When a compound of the above group B is applied for foliar treatmentduring a crop growing period, it is applied preferably 10 to 120 daysafter sowing crop seeds and more preferably 21 to 90 days after sowingcrop seeds. At this time, one compound selected from the compounds ofthe group B may be only applied or a plurality of compounds selectedfrom the compounds of the group B may be applied for the treatment. Whenthe plurality of compounds is applied, a plurality of formulations eachsingly containing one of these compounds may be used and at this time,these formulations are used either in combination or sequentially. Also,when the plurality of compounds is applied, a mixed formulationcontaining a plurality of compounds as active ingredients may be used.

The amount of the compound of the group B to be applied is generally 5to 5000 g, preferably 20 to 2000 g, and more preferably 500 to 1500 gper 10000 m². In this case, when applying the compound of the group B ina cultivation area, an adjuvant may be mixed in the compound to applythe mixture in the treatment.

Examples of the weed species that are the subjects to be controlled bysaflufenacil, trifludimoxazin, flumioxazin, or compound X in the presentinvention include, but not limited to, the following weeds:

Urticaceae: Urtica urens

Polygonaceae: Polygonum convolvulus, Polygonum lapathifolium, Polygonumpensylvanicum, Polygonum persicaria, Polygonum longisetum, Polygonumaviculare, Polygonum arenastrum, Polygonum cuspidatum, Rumex japonicus,Rumex crispus, Rumex obtusifolius, Rumex acetosa

Portulacaceae: Portulaca oleracea

Caryophyllaceae: Stellaria media, Stellaria aquatica, Cerastiumholosteoides, Cerastium glomeratum, Spergula arvensis, Silene gallica

Molluginaceae: Mollugo verticillata

Chenopodiaceae: Chenopodium album, Chenopodium ambrosioides, Kochiascoparia, Salsola kali, Atriplex spp.

Amaranthaceae: Amaranthus retroflexus, Amaranthus viridis, Amaranthuslividus, Amaranthus spinosus, Amaranthus hybridus, Amaranthus palmeri,Amaranthus patulus, waterhemp (Amaranthus tuberculatus =Amaranthus rudis=Amaranthus tamariscinus), Amaranthus blitoides, Amaranthus deflexus,Amaranthus quitensis, Alternanthera philoxeroides, Alternantherasessilis, Alternanthera tenella

Papaveraceae: Papaver rhoeas, Papaver dubium, Argemone mexicana

Brassicaceae: Raphanus raphanistrum, Raphanus sativus, Sinapis arvensis,Capsella bursa-pastoris, Brassica juncea, Brassica napus, Descurainiapinnata, Rorippa islandica, Rorippa sylvestris, Thlaspi arvense, Myagrumrugosum, Lepidium virginicum, Coronopus didymus

Capparaceae: Cleome affinis

Fabaceae: Aeschynomene indica, Aeschynomene rudis, Sesbania exaltata,Cassia obtusifolia, Cassia occidentalis, Desmodium tortuosum, Desmodiumadscendens, Desmodium illinoense, Trifolium repens, Pueraria lobata,Vicia angustifolia, Indigofera hirsuta, Indigofera truxillensis, Vignasinensis

Oxalidaceae: Oxalis corniculata, Oxalis strica, Oxalis oxyptera

Geraniaceae: Geranium carolinense, Erodium cicutarium

Euphorbiaceae: Euphorbia helioscopia, Euphorbia maculata, Euphorbiahumistrata, Euphorbia esula, Euphorbia heterophylla, Euphorbiabrasiliensis, Acalypha australis, Croton glandulosus, Croton lobatus,Phyllanthus corcovadensis, Ricinus communis

Malvaceae: Abutilon theophrasti, Sida rhombiforia, Sida cordifolia, Sidaspinosa, Sida glaziovii, Sida santaremnensis, Hibiscus trionum, Anodacristata, Malvastrum coromandelianum

Onagraceae: Ludwigia epilobioides, Ludwigia octovalvis, Ludwigiadecurre, Oenothera biennis, Oenothera laciniata

Sterculiaceae: Waltheria indica

Violaceae: Viola arvensis, Viola tricolor

Cucurbitaceae: Sicyos angulatus, Echinocystis lobata, Momordicacharantia

Lythraceae: Ammannia multiflora, Ammannia auriculata, Ammannia coccinea,Lythrum salicaria, Rotala indica

Elatinaceae: Elatine triandra, Elatine californica

Apiaceae: Oenanthe javanica, Daucus carota, Conium maculatum

Araliaceae: Hydrocotyle sibthorpioides, Hydrocotyle ranunculoides

Ceratophyllaceae: Ceratophyllum demersum

Cabombaceae: Cabomba caroliniana

Haloragaceae: Myriophyllum aquaticum, Myriophyllum verticillatum,Myriophyllum spicatum, Myriophyllum heterophyllum, and the like

Sapindaceae: Cardiospermum halicacabum

Primulaceae: Anagallis arvensis

Asclepiadaceae: Asclepias syriaca, Ampelamus albidus

Rubiaceae: Galium aparine, Galium spurium var. echinospermon, Spermacocelatifolia, Richardia brasiliensis, Borreria alata

Convolvulaceae: Ipomoea nil, Ipomoea hederacea, Ipomoea purpurea,Ipomoea hederacea var. integriuscula, Ipomoea lacunosa, Ipomoea triloba,Ipomoea acuminata, Ipomoea hederifolia, Ipomoea coccinea, Ipomoeaquamoclit, Ipomoea grandifolia, Ipomoea aristolochiafolia, Ipomoeacairica, Convolvulus arvensis, Calystegia hederacea, Calystegiajaponica, Merremia hedeacea, Merremia aegyptia, Merremia cissoides,Jacquemontia tamnifolia

Boraginaceae: Myosotis arvensis

Lamiaceae: Lamium purpureum, Lamium amplexicaule, Leonotis nepetaefolia,Hyptis suaveolens, Hyptis lophanta, Leonurus sibiricus, Stachys arvensis

Solanaceae: Datura stramonium, Solanum nigrum, Solanum americanum,Solanum ptycanthum, Solanum sarrachoides, Solanum rostratum, Solanumaculeatissimum, Solanum sisymbriifolium, Solanum carolinense, Physalisangulata, Physalis subglabrata, Nicandra physaloides

Scrophulariaceae: Veronica hederaefolia, Veronica persica, Veronicaarvensis, Lindernia procumbens, Lindernia dubia, Lindernia angustifolia,Bacopa rotundifolia, Dopatrium junceum, Gratiola japonica

Plantaginaceae: Plantago asiatica, Plantago lanceolata, Plantago major,Callitriche palustris

Asteraceae: Xanthium pensylvanicum, Xanthium occidentale, Xanthiumitalicum, Helianthus annuus, Matricaria chamomilla, Matricariaperforata, Chrysanthemum segetum, Matricaria matricarioides, Artemisiaprinceps, Artemisia vulgaris, Artemisia verlotorum, Solidago altissima,Taraxacum officinale, Galinsoga ciliata, Galinsoga parviflora, Seneciovulgaris, Senecio brasiliensis, Senecio grisebachii, Conyza bonariensis,Conyza smatrensis, Conyza canadensis, ragweed (Ambrosiaartemisiaefolia), Ambrosia trifida, Bidens tripartita, Bidens pilosa,Bidens frondosa, Bidens subalternans, Cirsium arvense, Cirsium vulgare,Silybum marianum, Carduus nutans, Lactuca serriola, Sonchus oleraceus,Sonchus asper, Wedelia glauca, Melampodium perfoliatum, Emiliasonchifolia, Tagetes minuta, Blainvillea latifolia, Tridax procumbens,Porophyllum ruderale, Acanthospermum australe, Acanthospermum hispidum,Cardiospermum halicacabum, Ageratum conyzoides, Eupatorium perfoliatum,Eclipta alba, Erechtites hieracifolia, Gamochaeta spicata, Gnaphaliumspicatum, Jaegeria hirta, Parthenium hysterophorus, Siegesbeckiaorientalis, Soliva sessilis, Eclipta prostrata, Eclipta alba, Centipedaminima

Alismataceae: Sagittaria pygmaea, Sagittaria trifolia, Sagittariasagittifolia, Sagittaria montevidensis, Sagittaria aginashi, Alismacanaliculatum, Alisma plantago-aquatica

Limnocharitaceae: Limnocharis flava

Hydrocharitaceae: Limnobium spongia, Hydrilla verticillata, Najasguadalupensis

Araceae: Pistia stratiotes

Lemnaceae: Lemna aoukikusa, Spirodela polyrhiza, Wolffia spp.

Potamogetonaceae: Potamogeton distinctus, Potamogeton crispus,Potamogeton illinoensis, Stuckenia pectinata, and the like

Liliaceae: Allium canadense, Allium vineale, Allium macrostemon

Pontederiaceae: Eichhornia crassipes, Heteranthera limosa, Monochoriakorsakowii, Monochoria vaginalis

Commelinaceae: Commelina communis, Commelina bengharensis, Commelinaerecta, Murdannia keisak

Poaceae: Echinochloa crus-galli, Echinochloa oryzicola, Echinochloacrus-galli var formosensis, Echinochloa oryzoides, Echinochloa colona,Echinochloa crus-pavonis, Setaria viridis, Setaria faberi, Setariaglauca, Setaria geniculata, Digitaria ciliaris, Digitaria sanguinalis,Digitaria horizontalis, Digitaria insularis, Eleusine indica, Poa annua,Poa trivialis, Poa pratensis, Alopecurus aequalis, Alopecurusmyosuroides, Avena fatua, Sorghum halepense, Sorghum vulgare, Agropyronrepens, Lolium multiflorum, Lolium perenne, Lolium rigidum, Bromuscatharticus, Bromus sterilis, Bromus japonicus, Bromus secalinus, Bromustectorum, Hordeum jubatum, Aegilops cylindrica, Phalaris arundinacea,Phalaris minor, Apera spica-venti, Panicum dichotomiflorum, Panicumtexanum, Panicum maximum, Brachiaria platyphylla, Brachiariaruziziensis, Brachiaria plantaginea, Brachiaria decumbens, Brachiariabrizantha, Brachiaria humidicola, Cenchrus echinatus, Cenchruspauciflorus, Eriochloa villosa, Pennisetum setosum, Chloris gayana,Chloris virgata, Eragrostis pilosa, Rhynchelitrum repens, Dactylocteniumaegyptium, Ischaemum rugosum, Isachne globosa, Oryza sativa, Paspalumnotatum, Paspalum maritimum, Paspalum distichum, Pennisetumclandestinum, Pennisetum setosum, Rottboellia cochinchinensis,Leptochloa chinensis, Leptochloa fascicularis, Leptochloa filiformis,Leptochloa panicoides, Leersia japonica, Leersia sayanuka, Leersiaoryzoides, Glyceria leptorrhiza, Glyceria acutiflora, Glyceria maxima,Agrostis gigantea, Agrostis stolonifera, Cynodon dactylon, Dactylisglomerata, Eremochloa ophiuroides, Festuca arundinacea, Festuca rubra,Imperata cylindrica, Miscanthus sinensis, Panicum virgatum, Zoysiajaponica

Cyperaceae: Cyperus microiria, Cyperus iria, Cyperus compressus, Cyperusdifformis, Cyperus flaccidus, Cyperus globosus, Cyperus nipponics,Cyperus odoratus, Cyperus serotinus, Cyperus rotundus, Cyperusesculentus, Kyllinga gracillima, Kyllinga brevifolia, Fimbristylismiliacea, Fimbristylis dichotoma, Eleocharis acicularis, Eleochariskuroguwai, Schoenoplectiella hotarui, Schoenoplectiella juncoides,Schoenoplectiella wallichii, Schoenoplectiella mucronatus,Schoenoplectiella triangulatus, Schoenoplectiella nipponicus,Schoenoplectiella triqueter, Bolboschoenus koshevnikovii, Bolboschoenusfluviatilis

Equisetaceae: Equisetum arvense, Equisetum palustre

Salviniaceae: Salvinia natans

Azollaceae: Azolla japonica, Azolla imbricata

Marsileaceae: Marsilea quadrifolia.

Other weeds: filamentous algae (Pithophora and Cladophora), mosses,bryophyte, hornwort, cyanobacteria, bracken, and suckers of perennialcrops (for example, pome fruits, stone fruits, berries, tree nuts,citrus fruits, hop, and grape).

In the present invention, specific PPO inhibitor resistant weeds whichare control targets each have one or more mutations selected from anArg128Met mutation, Arg128Gly mutation, Arg128His mutation, andGly399Ala mutation. PPO means protoporphyrinogen oxidase. Although PPO1and PPO2 are usually included in PPO of weeds, the above mutation may becontained in any one of PPO1 and PPO2 or both. The case where themutation is contained in PPO2 is preferable.

For example, Arg128Met means that the relevant mutation exists on the128th (the number is standardized in PPO2 of waterhemp) amino acid. InPPO2 of a ragweed, the relevant mutation corresponds to the 98th aminoacid (Weed Science 60, 335-344) as known by the notation “Arg98Leu”. Thenotation “Arg98” in this case is equal to “Arg128” defined in thepresent invention. The Arg128Met mutation and Arg128G1y mutation in PPOof a weed that is a control target in the present invention are known inthe case of Amaranthus palmeri (Pest Management Science 73, 1159-1563),the Arg128His mutation is known in the case of Lolium rigidum (WSSAannual meeting, 2018), and the Gly399Ala mutation is known in the caseof Amaranthus palmeri (WSSA annual meeting, 2018). Although resistantweeds disclosed in these reports are efficiently controlled in thepresent invention, the weed in the present invention is not limited tothe above resistant weeds. In other words, other weeds having therelevant amino acid mutations are controlled in the same manner. Notonly Amaranthus palmeri having an Arg128Met mutation, Arg128Glymutation, Arg128His mutation, or Gly399Ala mutation, but also, forexample, waterhemp having the same mutations, ragweed having the samemutations, Lolium rigidum having the same mutations, Lolium multiflorumhaving the same mutations, and Euphorbia heterophylla having the samemutations are efficiently controlled.

In the method of controlling resistant weeds according to the presentinvention, the above PPO inhibitor resistant weeds can be controlled byapplying saflufenacil, trifludimoxazin, flumioxazin, or compound X in acrop cultivation area. In this case, the above PPO inhibitor resistantweeds are not resistant to the compound to be applied. In the PPOinhibitor resistant weeds, there is no particular limitation to anintraspecific variation in traits other than PPC inhibitor resistance.In other words, the above PPO inhibitor resistant weeds may also includethose which are deteriorated in sensitivity to specific herbicides otherthan the PPO inhibitor and have traits resistant to these herbicides.The deterioration in sensitivity and the resistance may be due to eithera mutation at a target site (target-site mutation) or a factor that isnot a mutation at a target site (non target-site mutation). Examples ofthe non target-site mutation include increase in metabolism,malabsorption, transition failure, and external excretion. Examples ofthe factor promoting metabolism include those caused by increase in theactivities of metabolases such as cytochrome P450 monooxygenase,allylacylamidase, esterase, and glutathione S-transferase. Examples offactor of the external excretion include those caused by transportationinto vacuoles which is driven by an ABC transporter. Examples of thetarget-site mutations include any one of the followings in ALS genes orthose substituted with a plurality of amino acids: Ala122Thr, Ala122Val,Ala122Tyr, Pro197Ser, Pro197His, Pro197Thr, Pro197Arg, Pro197Leu,Pro197G1n, Pro197Ala, Pro19711e, Ala205Val, Ala205Phe, Asp376Glu,Arg377His, Trp574Leu, Trp574Gly, Trp574Met, Ser653Thr, Ser653Thr,Ser653Asn, Ser63511e, Gly654Glu, and Gly645Asp. Similarly, examples ofthe deterioration in the sensitivity of weeds caused by target-sitemutations include any one of the followings in ACCase genes or thosesubstituted with a plurality of amino acids: Ile1781Leu, Ile1781Val,Ile1781Thr, Trp1999Cys, Trp1999Leu, Ala2004Val, Trp2027Cys, Ile2041Asn,11e2041Val, Asp2078Gly, and Cys2088Arg. Similarly, examples of thedeterioration in the sensitivity of weeds caused by target-sitemutations include those substituted with amino acids in EPSP genes suchas Thr102Ile, Pro106Ser, Pro106Ala, and Pro106Leu. Particularly,glyphosate resistant weeds such as Eleusine indica, Lolium multiflorum,Lolium rigidum, waterhemp, and Echinochloa colona containing one or aplurality of these mutations (for example, a double mutation ofThr102Ile and Pro106Ser) are efficiently controlled even if they furtherhave the above mutations in PPO. Similarly, examples of thedeterioration in the sensitivity of weeds caused by mutations at atarget site include those increased in the number of copies of EPSPgenes, and weeds such as Amaranthus palmeri, waterhemp, and Kochiascoparia which each have the above mutation and glyphosate resistanceare efficiently controlled even if they each further have the abovemutations in PPO. Conyza canadensis, Conyza sumatrensis, and Conyzabonariensis which are resistant to glyphosate in which an ABCtransporter is involved are efficiently controlled even if they eachhave the above mutations in PPO.

In the method of controlling resistant weeds according to the presentinvention, one or more different herbicides, plant growth regulators,and safeners may be used in combination with saflufenacil,trifludimoxazin, flumioxazin, or compound X. Here, the description “usedin combination with” includes blending (tank mixing), mixing(premixing), and sequential treatments and in the case of the sequentialtreatments, the order is not particularly limited.

Examples of the above herbicides, plant growth regulators, and safenersinclude the following compounds:

Herbicides: 2,3,6-TBA (2,3,6-trichlorobenzoic acid),2,3,6-TBA-dimethylammonium, 2,3,6-TBA lithium salt, 2,3,6-TBA potassiumsalt, 2,3,6-TBA sodium salt, 2,4-D, 2,4-D choline salt, 2,4-D BAPMA salt(2,4-D N,N-bis(3-aminopropyl)methylamine salt), 2,4-D-2-butoxypropyl,2,4-D-2-ethylhexyl, 2,4-D-3-butoxypropyl, 2,4-D ammonium, 2,4-D-butotyl,2,4-D-butyl, 2,4-D-diethylammonium, 2,4-D-dimethylammonium, 2,4-Ddiolamine salt, 2,4-D-dodecylammonium, 2,4-D-ethyl,2,4-D-heptylammonium, 2,4-D-isobutyl, 2,4-D-isooctyl, 2,4-D-isopropyl,2,4-D-isopropylammonium, 2,4-D-lithium salt, 2,4-D-meptyl, 2,4-D-methyl,2,4-D-octyl, 2,4-D-pentyl, 2,4-D-propyl, 2,4-D-sodium salt,2,4-D-tefuryl, 2,4-D-tetradecylammonium, 2,4-D-triethylammonium,2,4-D-tris(2-hydroxypropyl)ammonium, 2,4-D-trolamine salt, 2,4-DB,2,4-DB choline salt, 2,4-DB BAPMA salt (2,4-DBN,N-bis(3-aminopropyl)methylamine salt), 2,4-DB-butyl,2,4-DB-dimethylammonium, 2,4-DB-isoctyl, 2,4-DB-potassium salt, 2,4-DBsodium salt, acetochlor, acifluorfen, acifluorfen-sodium salt,aclonifen, ACN(2-amino-3-chloronaphthalene-1,4-dione), alachlor,allidochlor, alloxydim, ametryn, amicarbazone, amidosulfuron,aminocyclopyrachlor, aminocyclopyrachlor-methyl,aminocyclopyrachlor-potassium salt, aminopyralid (aminopyralid),aminopyralid choline salt, aminopyralid-potassium salt,aminopyralid-tris(2-hydroxypropyl)ammonium, amiprophos-methyl, amitrole,anilofos, asulam, atrazine, azafenidin, azimsulfuron, beflubutamid,benazolin-ethyl, bencarbazone, benfluralin, benfuresate, bensulfuron,bensulfuron-methyl, bensulide, bentazon, benthiocarb, benzfendizone,benzobicyclon, benzofenap, benzthiazuron, bialafos (bialafos orbialaphos), bicyclopyrone, bifenox, bispyribac, bispyribac-sodium salt,bromacil, bromobutide, bromofenoxim, bromoxynil, bromoxynil-octanoate,butachlor, butafenacil, butamifos, butralin, butroxydim, butylate,cafenstrole, carbetamide, carfentrazone, carfentrazone-ethyl,chlomethoxyfen, chloramben, chloridazon, chlorimuron, chlorimuron-ethyl,chlorobromuron, chlorotoluron, chloroxuron, chlorpropham, chlorsulfuron,chlorthal-dimethyl, chlorthiamide, cinidon, cinidon-ethyl, cinmethylin,cinosulfuron, clethodim, clodinafop, clodinafop-propargyl, clomazone,clomeprop, clopyralid, clopyralid choline salt, clopyralid-methyl,clopyralid-olamine, clopyralid-potassium salt, clopyralid-tris(2-hydroxypropyl)ammonium, cloransulam, cloransulam-methyl, cumyluron,cyanazine, cyclopyranil, cycloate, cyclopyrimorate, cyclosulfamuron,cycloxydim, cyhalofop, cyhalofop-butyl, daimuron, dalapon, dazomet,desmedipham, desmetryn, di-allate, dicamba, dicamba choline salt,dicamba BAPMA salt (dicamba N,N-bis(3-aminopropyl)methylamine salt),dicamba-trolamine salt, dicamba-diglycolamine salt,dicamba-dimethylammonium, dicamba-diolamine salt,dicamba-isopropylammonium, dicamba-methyl, dicamba-olamine salt,dicamba-potassium salt, dicamba-sodium salt, dichlobenil, dichlorprop,dichlorprop choline salt), dichlorprop BAPMA salt (dichlorpropN,N-bis(3-aminopropyl)methylamine salt), dichlorprop-2-ethylhexyl,dichlorprop-butotyl, dichlorprop-dimethylammonium,dichlorprop-ethylammonium, dichlorprop-isoctyl, dichlorprop-methyl,dichlorprop-P, dichlorprop-P choline salt, dichlorprop-P BAPMA salt(dichlorprop-P N,N-bis(3-aminopropyl)methylamine salt),dichlorprop-P-2-ethylhexyl, dichlorprop-P-dimethylammonium,dichlorprop-potassium, dichlorprop-sodium salt, diclofop,diclofop-methyl, diclosulam, difenoxuron, difenzoquat, diflufenican,diflufenzopyr, diflufenzopyr-sodium salt, dimefuron, dimepiperate,dimethachlor, dimethametryn, dimethenamid, dimethenamid-P, dimepiperate,dinitramine, dinoseb, dinoterb, diphenamid, diquat, diquat-dibromide,DSMA (disodium methylarsonate), dithiopyr, diuron, DNOC(2-methyl-4,6-dinitrophenol), esprocarb, ethalfluralin, ethametsulfuron,ethametsulfuron-methyl, ethidimuron, ethofumesate, ethoxyfenethyl,ethoxysulfuron, etobenzanid, fenoxaprop, fenoxaprop-ethyl, fenoxaprop-P,fenoxaprop-P-ethyl, fenoxasulfone, fenquinotrione, fentrazamide,fenuron, flamprop-M, flazasulfuron, florasulam, florpyrauxifen,florpyrauxifen-benzyl, fluazifop, fluazifop-butyl, fluazifop-P,fluazifop-P-butyl, fluazolate, flucarbazone, flucarbazone-sodium salt,flucetosulfuron, flufenacet, flufenpyr, flufenpyr-ethyl, flumetsulam,flumetsulam, flumiclorac, flumiclorac-pentyl, fluometuron,fluoroglycofen-ethyl, flupoxam, flupropanate, flupyrsulfuron,flupyrsulfuron-methyl-sodium, flurenol, fluridone, flurochloridone,fluroxypyr, fluroxypyr-meptyl, flurtamone, fluthiacet,fluthiacet-methyl, fomesafen, fomesafen-sodium, foramsulfuron, fosamine,glufosinate, glufosinate-ammonium salt, glufosinate-P,glufosinate-P-ammonium salt, glufosinate-P-sodium salt, glyphosate,glyphosate choline salt, glyphosate guanidine derivative salts,glyphosate isopropylamine salt, glyphosate BAPMA salt (glyphosateN,N-bis(3-aminopropyl)methylamine salt), glyphosate-ammonium salt,glyphosate-diammonium salt, glyphosate-potassium salt, glyphosate-sodiumsalt, glyphosate-trimethylsulfonium salt, halauxifen, halauxifen-methyl,halosafen, halosulfuron, halosulfuron-methyl, haloxyfop,haloxyfop-etotyl, haloxyfop-methyl, haloxyfop-P, haloxyfop-P-etotyl,haloxyfop-P-methyl, hexazinone, imazamethabenz, imazamethabenz-methyl,imazamox, imazamox-ammonium salt, imazapic, imazapic-ammonium salt,imazapyr, imazapyr-isopropyl ammonium salt, imazaquin,imazaquin-ammonium salt, imazethapyr, imazethapyr-ammonium salt,imazosulfuron, indanofan, indaziflam, iodosulfuron,iodosulfuron-methyl-sodium, iofensulfuron, iofensulfuron-sodium,ioxynil, ioxynil-octanoate, ipfencarbazone, isoproturon, isouron,isoxaben, isoxachlortole, isoxaflutole, lactofen, lenacil, linuron,maleic acid hydrazide, MCPA (2-(4-chloro-2-methylphenoxy)acetic acid),MCPA choline salt, MCPA BAPMA salt (MCPAN,N-bis(3-aminopropyl)methylamine salt), MCPA 2-ethylhexyl, MCPA butotyl(MCPA-butotyl), MCPA butyl, MCPA-dimethylammonium, MCPA diolamine salt,MCPA-ethyl, MCPA isobutyl, MCPA-isoctyl, MCPA-isopropyl, MCPA-methyl,MCPA-olamine salt, MCPA-sodium salt, MCPA-trolamine salt, MCPB(4-(4-chloro-2-methylphenoxy)butanoic acid), MCPB choline salt, MCPBBAPMA salt (MCPB N,N-bis(3-aminopropyl)methylamine salt), MCPB-ethyl,MCPB-methyl, MCPB-sodium salt, mecoprop, mecoprop choline salt, mecopropBAPMA salt (mecoprop N,N-bis(3-aminopropyl)methylamine salt),mecoprop-2-ethylhexyl, mecoprop-dimethylammonium, mecoprop-diolaminesalt, mecoprop-ethadyl, mecoprop-isoctyl, mecoprop-methyl,mecoprop-potassium salt, mecoprop-sodium salt, mecoprop-trolamine salt,mecoprop-P, mecoprop-P choline salt, mecoprop-P-2-ethylhexyl,mecoprop-P-dimethylammonium, mecoprop-P-isobutyl, mecoprop-P-potassiumsalt, mefenacet, mesosulfuron, mesosulfuron-methyl, mesotrione, metam,metamifop, metamitron, metazachlor, metazosulfuron, methabenzthiazuron,methiozolin, methyl-daymuron, metobromuron, metolachlor, metosulam,metoxuron, metribuzin, metsulfuron, metsulfuron-methyl, molinate,monolinuron, naproanilide, napropamide, napropamide-M, naptalam,neburon, nicosulfuron, norflurazon, oleic acid, orbencarb,orthosulfamuron, oryzalin, oxadiargyl, oxadiazon, oxasulfuron,oxaziclomefone, oxyfluorfen, paraquat, paraquat-dichloride, pebulate,pelargonic acid, pendimethalin, penoxsulam, pentanochlor, pentoxazone,pethoxamid, phenisopham, phenmedipham, picolinafen, pinoxaden,piperophos, pretilachlor, primisulfuron, primisulfuron-methyl,prodiamine, profluazol, profoxydim, prometon, prometryn, propachlor,propanil, propaquizafop, propazine, propham, propisochlor,propoxycarbazone, propoxycarbazone-sodium salt, propyrisulfuron,propyzamide, prosulfocarb, prosulfuron, pyraclonil, pyraflufen-ethyl,pyrasulfotole, pyrazolynate, pyrazosulfuron, pyrazosulfuron-ethyl,pyrazoxyfen, pyribenzoxim, pyributicarb, pyridafol, pyridate,pyriftalid, pyriminobac, pyriminobac-methyl, pyrimisulfan, pyrithiobac,pyrithiobac-sodium salt, pyroxasulfone, pyroxsulam, quinclorac,quinmerac, quizalofop, quizalofop-ethyl, quizalofop-tefuryl,quizalofop-P, quizalofop-P-ethyl, quizalofop-P-tefuryl, rimsulfuron,sethoxydim, EPTC (S-ethyl N,N-dipropylcarbamothioate), siduron,simazine, simetryn, S-metolachlor, MSMA (sodium hydrogenmethylarsonate), sulcotrione, sulfentrazone, sulfometuron,sulfometuron-methyl, sulfosulfuron, swep, TCA (2,2,2-trichloroaceticacid), tebutam, tebuthiuron, tefuryltrione, tembotrione, tepraloxydim,terbacil, terbumeton, terbuthylazine, terbutryn, thaxtomin A,thenylchlor, thiazopyr, thidiazimin, thiencarbazone,thiencarbazone-methyl, thifensulfuron, thifensulfuron-methyl,tiafenacil, tiocarbazil, tolpyralate, topramezone, tralkoxydim,triafamone, tri-allate, triasulfuron, triaziflam, tribenuron,tribenuron-methyl, triclopyr, triclopyr-butotyl, triclopyr-ethyl,triclopyr-triethylammonium, tridiphane, trietazine, trifloxysulfuron,trifloxysulfuron-sodium salt, trifluralin, triflusulfuron,triflusulfuron-methyl, tritosulfuron, vernolate, and ethyl[(3-{2-chloro-4-fluoro-5-[3-methyl-4-(trifluoromethyl)-2,6-dioxo-1,2,3,6-tetrahydropyrimidin-1-yl]phenoxy}pyridin-2-yl)oxy]acetate(353292-31-6).

Safener: allidochlor, benoxacor, cloquintocet, cloquintocet-mexyl,cyometrinil, cyprosulfamide, dichlormid, dicyclonone, dimepiperate,disulfoton, daiymuron, fenchlorazole, fenchlorazole-ethyl, fenclorim,flurazole, furilazole, fluxofenim, hexim, isoxadifen, isoxadifen-ethyl,mecoprop, mefenpyr, mefenpyr-ethyl, mefenpyr-diethyl, mephenate,metcamifen, oxabetrinil, 1,8-naphthalic anhydride, 1,8-octamethylenediamine, AD-67 (4-(dichloroacetyl)-1-oxa-4-azaspiro [4.5] decane), MCPA(2-(4-chloro-2-methylphenoxy)acetic acid), CL-304415(4-carboxy-3,4-dihydro-2H-1-benzopyran-4-acetic acid), CSB(1-bromo-4-[(chloromethyl)sulfonyl]benzene), DKA-24(2,2-dichloro-N-[2-oxo-2-(2-propenylamino)ethyl]-N-(2-propenyl)acetamide),MG191 (2-(dichloromethyl)-2-methyl-1,3-dioxolane), MG-838 (2-propenyl1-oxa-4-azaspiro[4.5]decane-4-carbodithioate), PPG-1292(2,2-dichloro-N-(1,3-dioxan-2-ylmethyl)-N-(2-propenyl)acetamide),R-28725 (3-(dichloroacetyl)-2,2-dimethyl-1,3-oxazolidine), R-29148(3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine), and TI-35(1-(dichloroacetyl)azepane).

Plant growth regulator: hymexazol, paclobutrazol, uniconazole,uniconazole-P, inabenfide, prohexadione-calcium, 1-methylcyclopropene,and trinexapac.

As the herbicide which may be used in combination with saflufenacil,trifludimoxazin, flumioxazin, or compound X in the present invention,glyphosate potassium salt, glyphosate guanidine salt, glyphosatedimethylamine salt, glyphosate monoethanolamine salt, glufosinateammonium salt, glyphosate isopropylammonium salt, flumiclorac-pentyl,clethodim, lactofen, S-metolachlor, metribuzin, flufenacet,nicosulfuron, rimsulfuron, acetochlor, mesotrione, isoxaflutole,chlorimuron-ethyl, thifensulfuron-methyl, cloransulam-methyl,imazethapyr-ammonium salt, and metribuzin are particularly preferable.

As the safener which may be used in combination with saflufenacil,trifludimoxazin, flumioxazin, or compound X in the present invention,cyprosulfamide, benoxacor, dichlormid, and furilazole are particularlypreferable.

Examples of the combinations of saflufenacil, trifludimoxazin,flumioxazin, or compound X and herbicide and/or safener are given below,but not limited to the following combinations. The ratio by weight ofeach compound to saflufenacil, trifludimoxazin, flumioxazin, or compoundX to be combined is usually 0.01 to 1000 times, preferably 0.1 to 100times, and more preferably 1 to 10 times.

Specific combinations are disclosed below. Also, those in which thecompound X is replaced with trifludimoxazin are disclosed at the sametime. Also, those in which the compound X is replaced with saflufenacilare disclosed at the same time. Also, those in which the compound X isreplaced with flumioxazin are disclosed at the same time. Also, those inwhich the compound X is replaced with the compound X +flumioxazin aredisclosed at the same time. Also, those in which the compound X isreplaced with saflufenacil+trifludimoxazin are disclosed at the sametime:

-   Combination of the compound X and quinclorac;-   Combination of the compound X and quinmerac;-   Combination of the compound X and bromoxynil;-   Combination of the compound X and bromoxynil-octanoate;-   Combination of the compound X and dichlobenil;-   Combination of the compound X and methiozolin;-   Combination of the compound X and ioxynil;-   Combination of the compound X and ioxynil-octanoate;-   Combination of the compound X and di-allate;-   Combination of the compound X and butylate;-   Combination of the compound X and tri-allate;-   Combination of the compound X and phenmedipham;-   Combination of the compound X and chlorpropham;-   Combination of the compound X and desmedipham;-   Combination of the compound X and asulam;-   Combination of the compound X and phenisopham;-   Combination of the compound X and benthiocarb;-   Combination of the compound X and molinate;-   Combination of the compound X and esprocarb;-   Combination of the compound X and pyributicarb;-   Combination of the compound X and prosulfocarb;-   Combination of the compound X and orbencarb;-   Combination of the compound X and EPTC;-   Combination of the compound X and dimepiperate;-   Combination of the compound X and swep;-   Combination of the compound X and propachlor;-   Combination of the compound X and metazachlor;-   Combination of the compound X and alachlor;-   Combination of the compound X and acetochlor;-   Combination of the compound X and metolachlor;-   Combination of the compound X and S-metolachlor;-   Combination of the compound X and butachlor;-   Combination of the compound X and pretilachlor;-   Combination of the compound X and thenylchlor;-   Combination of the compound X and aminocyclopyrachlor;-   Combination of the compound X and aminocyclopyrachlor-methyl;-   Combination of the compound X and aminocyclopyrachlor-potassium;-   Combination of the compound X and trifluralin;-   Combination of the compound X and pendimethalin;-   Combination of the compound X and ethalfluralin;-   Combination of the compound X and benfluralin;-   Combination of the compound X and prodiamine;-   Combination of the compound X and simazine;-   Combination of the compound X and atrazine;-   Combination of the compound X and propazine;-   Combination of the compound X and cyanazine;-   Combination of the compound X and ametryn;-   Combination of the compound X and simetryn;-   Combination of the compound X and dimethametryn;-   Combination of the compound X and indaziflam;-   Combination of the compound X and triaziflam;-   Combination of the compound X and metribuzin;-   Combination of the compound X and hexazinone;-   Combination of the compound X and terbumeton;-   Combination of the compound X and terbuthylazine;-   Combination of the compound X and terbutryn;-   Combination of the compound X and trietazine;-   Combination of the compound X and isoxaben;-   Combination of the compound X and diflufenican;-   Combination of the compound X and diuron;-   Combination of the compound X and linuron;-   Combination of the compound X and metobromuron;-   Combination of the compound X and metoxuron;-   Combination of the compound X and monolinuron;-   Combination of the compound X and siduron;-   Combination of the compound X and fluometuron;-   Combination of the compound X and difenoxuron;-   Combination of the compound X and methyl-daymuron;-   Combination of the compound X and isoproturon;-   Combination of the compound X and isouron;-   Combination of the compound X and tebuthiuron;-   Combination of the compound X and benzthiazuron;-   Combination of the compound X and methabenzthiazuron;-   Combination of the compound X and propanil;-   Combination of the compound X and mefenacet;-   Combination of the compound X and clomeprop;-   Combination of the compound X and naproanilide;-   Combination of the compound X and bromobutide;-   Combination of the compound X and daimuron;-   Combination of the compound X and cumyluron;-   Combination of the compound X and diflufenzopyr;-   Combination of the compound X and etobenzanid;-   Combination of the compound X and bentazon;-   Combination of the compound X and tridiphane;-   Combination of the compound X and indanofan;-   Combination of the compound X and amitrole;-   Combination of the compound X and fenchlorazole-ethyl;-   Combination of the compound X and mefenpyr-diethyl;-   Combination of the compound X and benoxacor;-   Combination of the compound X and dichlormid;-   Combination of the compound X and cloquintocet-mexyl;-   Combination of the compound X and cyprosulfamide;-   Combination of the compound X and isoxadifen-ethyl;-   Combination of the compound X and clomazone;-   Combination of the compound X and maleic acid hydrazide;-   Combination of the compound X and pyridate;-   Combination of the compound X and chloridazon;-   Combination of the compound X and bromacil;-   Combination of the compound X and terbacil;-   Combination of the compound X and lenacil;-   Combination of the compound X and oxaziclomefone;-   Combination of the compound X and cinmethylin;-   Combination of the compound X and benfuresate;-   Combination of the compound X and cafenstrole;-   Combination of the compound X and flufenacet;-   Combination of the compound X and pyrithiobac;-   Combination of the compound X and pyrithiobac-sodium salt;-   Combination of the compound X and pyriminobac;-   Combination of the compound X and pyriminobac-methyl;-   Combination of the compound X and bispyribac;-   Combination of the compound X and bispyribac-sodium salt;-   Combination of the compound X and pyribenzoxim;-   Combination of the compound X and pyrimisulfan;-   Combination of the compound X and pyriftalid;-   Combination of the compound X and triafamone;-   Combination of the compound X and fentrazamide;-   Combination of the compound X and dimethenamid;-   Combination of the compound X and dimethenamid-P;-   Combination of the compound X and ACN;-   Combination of the compound X and dithiopyr;-   Combination of the compound X and triclopyr;-   Combination of the compound X and triclopyr-butotyl;-   Combination of the compound X and triclopyr-ammonium salt;-   Combination of the compound X and fluroxypyr;-   Combination of the compound X and fluroxypyr-meptyl;-   Combination of the compound X and thiazopyr;-   Combination of the compound X and aminopyralid;-   Combination of the compound X and aminopyralid-potassium salt;-   Combination of the compound X and aminopyralid-triisopanol-ammonium    salt;-   Combination of the compound X and clopyralid-olamine salt;-   Combination of the compound X and clopyralid-potassium salt;-   Combination of the compound X and clopyralid-triethyl-ammonium salt;-   Combination of the compound X and picloram-pottasium salt;-   Combination of the compound X and picloram-triisopanol-ammonium    salt;-   Combination of the compound X and dalapon;-   Combination of the compound X and chlorthiamid;-   Combination of the compound X and amidosulfuron;-   Combination of the compound X and azimsulfuron;-   Combination of the compound X and bensulfuron-methyl;-   Combination of the compound X and chlorimuron-ethyl;-   Combination of the compound X and cyclosulfamuron;-   Combination of the compound X and ethoxysulfuron;-   Combination of the compound X and flazasulfuron;-   Combination of the compound X and flucetosulfuron;-   Combination of the compound X and flupyrsulfuron-methyl-sodium;-   Combination of the compound X and foramsulfuron;-   Combination of the compound X and halosulfuron-methyl;-   Combination of the compound X and imazosulfuron;-   Combination of the compound X and mesosulfuron-methyl;-   Combination of the compound X and metazosulfuron;-   Combination of the compound X and nicosulfuron;-   Combination of the compound X and orthosulfamuron;-   Combination of the compound X and oxasulfuron;-   Combination of the compound X and primisulfuron-methyl;-   Combination of the compound X and propyrisulfuron;-   Combination of the compound X and pyrazosulfuron-ethyl;-   Combination of the compound X and rimsulfuron;-   Combination of the compound X and sulfometuron-methyl;-   Combination of the compound X and sulfosulfuron;-   Combination of the compound X and trifloxysulfuron-sodium salt;-   Combination of the compound X and chlorsulfuron;-   Combination of the compound X and cinosulfuron;-   Combination of the compound X and ethametsulfuron-methyl;

Combination of the compound X and iodosulfuron-methyl-sodium;

-   Combination of the compound X and iofensulfuron-sodium;-   Combination of the compound X and metsulfuron-methyl;-   Combination of the compound X and prosulfuron;-   Combination of the compound X and thifensulfuron-methyl;-   Combination of the compound X and triasulfuron;-   Combination of the compound X and tribenuron-methyl;-   Combination of the compound X and triflusulfuron-methyl;-   Combination of the compound X and tritosulfuron;-   Combination of the compound X and picolinafen;-   Combination of the compound X and beflubutamid;-   Combination of the compound X and norflurazon;-   Combination of the compound X and fluridone;-   Combination of the compound X and flurochloridone;-   Combination of the compound X and flurtamone;-   Combination of the compound X and benzobicyclon;-   Combination of the compound X and bicyclopyrone;-   Combination of the compound X and mesotrion;-   Combination of the compound X and sulcotrione;-   Combination of the compound X and tefuryltrione;-   Combination of the compound X and tembotrione;-   Combination of the compound X and isoxachlortole;-   Combination of the compound X and isoxaflutole;-   Combination of the compound X and benzofenap;-   Combination of the compound X and pyrasulfotole;-   Combination of the compound X and pyrazolynate;-   Combination of the compound X and pyrazoxyfen;-   Combination of the compound X and topramezone;-   Combination of the compound X and tolpyralate;-   Combination of the compound X and lancotrion-sodium salt;-   Combination of the compound X and flupoxam;-   Combination of the compound X and amicarbazone;-   Combination of the compound X and bencarbazone;-   Combination of the compound X and flucarbazone-sodium salt;-   Combination of the compound X and ipfencarbazone;-   Combination of the compound X and propoxycarbazone-sodium salt;-   Combination of the compound X and thiencarbazone-methyl;-   Combination of the compound X and cloransulam-methyl;-   Combination of the compound X and diclosulam;-   Combination of the compound X and florasulam;-   Combination of the compound X and flumetsulam;-   Combination of the compound X and metosulam;-   Combination of the compound X and penoxsulam;-   Combination of the compound X and pyroxsulam;-   Combination of the compound X and imazamethabenz-methyl;-   Combination of the compound X and imazamox-ammonium salt;-   Combination of the compound X and imazapic-ammonium salt;-   Combination of the compound X and imazapyr-isopropylammonium salt;-   Combination of the compound X and imazaquin-ammonium salt;-   Combination of the compound X and imazethapyr-ammonium salt;-   Combination of the compound X and clodinafop-propargyl;-   Combination of the compound X and cyhalofop-butyl;-   Combination of the compound X and diclofop-methyl;-   Combination of the compound X and fenoxaprop-ethyl;-   Combination of the compound X and fenoxaprop-P-ethyl;-   Combination of the compound X and fluazifop-butyl;-   Combination of the compound X and fluazifop-P-butyl;-   Combination of the compound X and haloxyfop-methyl;-   Combination of the compound X and haloxyfop-P-methyl;-   Combination of the compound X and propaquizafop;-   Combination of the compound X and quizalofop-ethyl;-   Combination of the compound X and quizalofop-P-ethyl;-   Combination of the compound X and alloxydim;-   Combination of the compound X and clethodim;-   Combination of the compound X and sethoxydim;-   Combination of the compound X and tepraloxydim;-   Combination of the compound X and tralkoxydim;-   Combination of the compound X and pinoxaden;-   Combination of the compound X and fenoxasulfone;-   Combination of the compound X and glufosinate;-   Combination of the compound X and glufosinate-ammonium salt;-   Combination of the compound X and glufosinate-P;-   Combination of the compound X and glufosinate-P-sodium salt;-   Combination of the compound X and bialafos;-   Combination of the compound X and anilofos;-   Combination of the compound X and bensulide;-   Combination of the compound X and butamifos;-   Combination of the compound X and paraquat;-   Combination of the compound X and paraquat-dichloride;-   Combination of the compound X and diquat;-   Combination of the compound X and diquat-dibromide;-   Combination of the compound X and halauxifen;-   Combination of the compound X and halauxifen-methyl;-   Combination of the compound X and florpyrauxifen;-   Combination of the compound X and florpyrauxifen-benzyl;-   Combination of the compound X and flumiclorac-pentyl;-   Combination of the compound X and fomesafen-sodium salt;-   Combination of the compound X and lactofen;-   Combination of the compound X and tiafenacil;-   Combination of the compound X and acifluorfen-sodium salt;-   Combination of the compound X and aclonifen;-   Combination of the compound X and bifenox;-   Combination of the compound X and chlomethoxyfen;-   Combination of the compound X and chlornitrofen;-   Combination of the compound X and ethoxyfen-ethyl;-   Combination of the compound X and fluorodifen;-   Combination of the compound X and fluoroglycofen-ethyl;-   Combination of the compound X and fluoronitrofen;-   Combination of the compound X and halosafen;-   Combination of the compound X and nitrofen;-   Combination of the compound X and nitrofluorfen;-   Combination of the compound X and oxyfluorfen;-   Combination of the compound X and cinidon-ethyl;-   Combination of the compound X and profluazol;-   Combination of the compound X and pyraclonil;-   Combination of the compound X and oxadiargyl;-   Combination of the compound X and oxadiazon;-   Combination of the compound X and pentoxazone;-   Combination of the compound X and fluazolate;-   Combination of the compound X and pyraflufen-ethyl;-   Combination of the compound X and benzfendizone;-   Combination of the compound X and butafenacil;-   Combination of the compound X and fluthiacet-methyl;-   Combination of the compound X and thidiazimin;-   Combination of the compound X and azafenidin;-   Combination of the compound X and carfentrazone-ethyl;-   Combination of the compound X and sulfentrazone;-   Combination of the compound X and flufenpyr-ethyl;-   Combination of the compound X and glyphosate;-   Combination of the compound X and glyphosate isopropylamine salt;-   Combination of the compound X and glyphosate-ammonium salt;-   Combination of the compound X and glyphosate-potassium salt;-   Combination of the compound X and glyphosate guanidine salt;-   Combination of the compound X and glyphosate dimethylamine salt;-   Combination of the compound X and glyphosate monoethanolamine salt;-   Combination of the compound X and MCPA;-   Combination of the compound X and MCPA-dimethylammonium salt;-   Combination of the compound X and MCPA-2-ethylhexyl ester;-   Combination of the compound X and MCPA-isoctyl ester;-   Combination of the compound X and MCPA-sodium salt;-   Combination of the compound X and MCPB;-   Combination of the compound X and mecoprop;-   Combination of the compound X and mecoprop-dimethylammonium salt;-   Combination of the compound X and mecoprop-diolamine salt;-   Combination of the compound X and mecoprop-ethadyl ester;-   Combination of the compound X and mecoprop-2-ethylhexyl ester;-   Combination of the compound X and mecoprop-isoctyl ester;-   Combination of the compound X and mecoprop-methyl ester;-   Combination of the compound X and mecoprop-potassium salt;-   Combination of the compound X and mecoprop-sodium salt;-   Combination of the compound X and mecoprop-trolamine salt;-   Combination of the compound X and mecoprop-P;-   Combination of the compound X and mecoprop-P-dimethylammonium salt;-   Combination of the compound X and mecoprop-P-2-ethylhexyl ester;-   Combination of the compound X and mecoprop-P-isobutyl ester;-   Combination of the compound X and mecoprop-P-potassium salt;-   Combination of the compound X and dichlorprop;-   Combination of the compound X and dichlorprop-butotyl ester;-   Combination of the compound X and dichlorprop-dimethylammonium salt;-   Combination of the compound X and dichlorprop-2-ethylhexyl ester;-   Combination of the compound X and dichlorprop-isoctyl ester;-   Combination of the compound X and dichlorprop-methyl ester;-   Combination of the compound X and dichlorprop-potassium salt;-   Combination of the compound X and dichlorprop-sodium salt;-   Combination of the compound X and dichlorprop-P;-   Combination of the compound X and dichlorprop-P-dimethylammonium    salt;-   Combination of the compound X and pyroxasulfone;-   Combination of the compound X and dicamba;-   Combination of the compound X and dicamba choline salt;-   Combination of the compound X and dicamba-BAPMA salt;-   Combination of the compound X and dicamba-trolamine salt;-   Combination of the compound X and dicamba-diglycolamine salt;-   Combination of the compound X and dicamba-dimethylammonium;-   Combination of the compound X and dicamba-diolamine salt;-   Combination of the compound X and dicamba-isopropylammonium;-   Combination of the compound X and dicamba-methyl;-   Combination of the compound X and dicamba-olamine salt;-   Combination of the compound X and dicamba-potassium salt;-   Combination of the compound X and dicamba-sodium salt;-   Combination of the compound X and pyroxasulfone;-   Combination of the compound X and 2,4-D;-   Combination of the compound X and 2,4-D choline salt;-   Combination of the compound X and 2,4-D BAPMA salt;-   Combination of the compound X and 2,4-D-2-butoxypropyl;-   Combination of the compound X and 2,4-D-2-ethylhexyl;-   Combination of the compound X and 2,4-D-ammonium;-   Combination of the compound X and 2,4-D-butotyl;-   Combination of the compound X and 2,4-D-butyl;-   Combination of the compound X and 2,4-D-diethylammonium;-   Combination of the compound X and 2,4-D-dimethylammonium;-   Combination of the compound X and 2,4-D-diolamine salt;-   Combination of the compound X and 2,4-D-dodecylammonium;-   Combination of the compound X and 2,4-D-ethyl;-   Combination of the compound X and 2,4-D-heptylammonium;-   Combination of the compound X and 2,4-D-isobutyl;-   Combination of the compound X and 2,4-D-isooctyl;-   Combination of the compound X and 2,4-D-isopropyl;-   Combination of the compound X and 2,4-D-isopropylammonium;-   Combination of the compound X and 2,4-D-lithium salt;-   Combination of the compound X and 2,4-D-meptyl;-   Combination of the compound X and 2,4-D-methyl;-   Combination of the compound X and 2,4-D-octyl;-   Combination of the compound X and 2,4-D-pentyl;-   Combination of the compound X and 2,4-D-propyl;-   Combination of the compound X and 2,4-D-sodium salt;-   Combination of the compound X and 2,4-D-tefuryl;-   Combination of the compound X and 2,4-D-tetradecylammonium;-   Combination of the compound X and 2,4-D-triethylammonium;-   Combination of the compound X and 2,4-D-tris(2-and-   Combination of the compound X and 2,4-D-trolamine salt.

In crop cultivation in the present invention, plant nutritional controlmade in usual crop cultivation can be applied. The fertilizing systemmay be either one based on Precision Agriculture or traditionallyuniform one. Also, nitrogen fixation bacteria or mycorrhizal fungi maybe inoculated in combination with seed treatment with the compound groupA.

EXAMPLES

The present invention will be explained by way of examples, which arehowever not mended to be limiting of the present invention.

Firstly, the standard of evaluation of herbicidal effects and phytotoxiceffects on crops which are described in Examples are shown below.

[Herbicidal Effects and phytotoxic Effects on Crops]

In the evaluation of herbicidal effects, the conditions of emergence andgrowing of a treated test weed upon measurement are rated between 0 to100 in which a measured test weed having no or almost no difference inthe conditions from an untreated plant is rated as “0” and a measuredtest weed that is completely killed or completely suppressed inemergence or growth is rated as “100”.

In the evaluation of the phytotoxic effects on crops, the case wherealmost no phytotoxic effect is observed is rated as “no phytotoxicity”,the case where a light level of phytotoxicity is observed is rated as“small”, the case where a mild level of phytotoxicity is observed israted as “middle”, and the case where a strong level of phtotoxicity isobserved is rated as “large”.

Example 1

Amaranthus palmeri having an Arg128Met mutation in PPO2 is sown in aplastic pot. On the same day, a compound X is applied to the surface ofsoil in an amount of 10, 20, 40, or 80 g/ha by spraying 200 L/ha of anaqueous dilution containing the compound K. Then, the weeds arecultivated in a greenhouse and soybean seeds are sown after 7 days, toexamine the effects on the weeds and phytotoxic effects on soybean after14 days. A valuable effect on the relevant Amaranthus palmeri isconfirmed.

Example 2

Amaranthus palmeri having an Arg128Met mutation in PPO2 and soybean aresown in a plastic pot. On the same day, a compound X is applied to thesurface of soil in an amount of 10, 20, 40, or 80 g/ha by spraying 200L/ha of an aqueous dilution containing the compound X. Then, the weedsand soybean are cultivated in a greenhouse to examine the effects on theweeds and phytotoxic effects on soybean after 21 days. A valuable effecton the relevant Amaranthus palmeri is confirmed.

Example 3

Amaranthus palmeri having an Arg128Met mutation in PPO2 and soybean aresown in a plastic pot. Then, the weeds and soybean are cultivated in agreenhouse. A compound X is applied in an amount of 10, 20, 40, or 80g/ha by spraying 200 L/ha of an aqueous dilution containing the compoundX to perform foliar treatment 21 days after the sowing. Then, the weedsand soybean are cultivated in a greenhouse to examine the effects on theweeds and phytotoxic effects on soybean 14 days after the treatment. Avaluable effect on the relevant Amaranthus palmeri is confirmed.

Examples 4 to 6

The same procedures as in Examples 1 to 3 are performed except that theabove Amaranthus palmeri used in Examples 1 to 3 is altered toAmaranthus palmeri having an Arg128Gly mutation in PPO2.

Examples 7 to 9

The same procedures as in Examples 1 to 3 are performed except that theabove Amaranthus palmeri used in Examples 1 to 3 is altered toAmaranthus palmeri having an Arg128His mutation in PPO2.

Examples 10 to 12

The same procedures as in Examples 1 to 3 are performed except that theabove Amaranthus palmeri used in Examples 1 to 3 is altered toAmaranthus palmeri having a Gly399Ala mutation in PPO2.

Examples 13 to 24

The same procedures as in Examples 1 to 12 are performed except that theabove Amaranthus palmeri used in Examples 1 to 12 is altered towaterhemp.

Examples 25 to 36

The same procedures as in Examples 1 to 12 are performed except that theabove Amaranthus palmeri used in Examples 1 to 12 is altered to ragweed.

Examples 37 to 48

The same procedures as in Examples 1 to 12 are performed except that theabove Amaranthus palmeri used in Examples 1 to 12 is altered to Loliumrigidum or Lolium multiflorum.

Examples 49 to 96

The same procedures as in Examples 1 to 48 are performed except that thecompound X used in Examples 1 to 48 is altered to Saflufenacil.

Examples 97 to 144

The same procedures as in Examples 1 to 48 are performed except that thetreatment using 10, 20, 40, or 80 g/ha of the compound X used inExamples 1 to 48 is altered to treatment using 20, 40, 80, or 160 g/haof trifludimoxazin.

Examples 145 to 192

The same procedures as in Examples 97 to 144 are performed except thatthe trifludimoxazin used in Examples 97 to 144 is altered toflumioxazin.

Examples 193 to 240

The same procedures as in Examples 1 to 48 are performed except that thetreatment using 10, 20, 40, or 80 g/ha of the compound X used inExamples 1 to 48 is altered to treatment using a combination ofsaflufenacil +trifludimoxazin in amounts of 10 +160 g/ha, 20 +160, 40+160, 80 +160 g/ha, 10 +80 g/ha, 20 +80, 40 +80, 80 +80 g/ha, 10 +40g/ha, 20 +40, 40 +40, 80 +40/ha, 10 +20 g/ha, 20 +20, 40 +20, or 80 +20g/ha.

Examples 241 to 288

The same procedures as in Examples 1 to 48 are performed except that thetreatment using 10, 20, 40, or 80 g/ha of the compound X used inExamples 1 to 48 is altered to treatment using a combination of thecompound X+flumioxazin in amounts of 10+160, 20+160, 40+160, 80+160,10+80, 20+80, 40+80, 80+80, 10+40, 20+40, 40+40, 80+40, 10+20, 20+20,40+20, or 80+2020 g/ha.

Examples 289 to 576

The same procedures as in Examples 1 to 288 are performed except thatRoundupPowerMax (glyphosate-potassium salt, 660 g/L) is further added inan amount of 32 fluid ounce/acre (1543 g/ha as a glyphosate-potassiumsalt) in each treatment of Examples 1 to 288.

Examples 577 to 864

The same procedures as in Examples 1 to 288 are performed except thatXtendiMax (dicamba diglycol amine salt, 480 g/L) is further added in anamount of 1 pint/acre (560 g/ha as a dicamba glycol amine salt) in eachtreatment of Examples 1 to 288.

Examples 865 to 1152

The same procedures as in Examples 1 to 288 are performed except that 32fluid ounce/acre of RoundupPowerMax and 1 pint/acre of XtendiMax arefurther added in each treatment of Examples 1 to 288.

Examples 1153 to 2304

The same procedures as in Examples 1 to 1152 are performed except thatsoybean used in Examples 1 to 1152 is altered to corn or cotton.

Examples 2305 to 4608

The same procedures as in Examples 1 to 2304 are performed except thatthe crop used in Examples 1 to 2304 is altered to a crop modified bygene recombination to have both a Roundup Ready 2 Xtend trait and a PPOinhibitor tolerant trait imparted by the presence of an exotic PPOdeteriorated in compatibility with an applied PPO inhibitor to a PPOexisting in the crop.

Example 4609

Plastic pots each having a diameter of 8 cm and a height of 7 cm werefilled with the soil and seedlings of Amaranthus palmeri having aGly399Ala mutation in PPO2 (originated from Mississippi, USA) wereplanted. After cultivation in a greenhouse for 31 days, an aqueous sprayliquid prepared by diluting a predetermined amount of a commercialsaflufenacil formulation (suspension concentrate containing 29.74% ofsaflufenacil, manufactured by BASF Corp. under the trade name ofSharpen) with water was sprayed uniformly over the weed using a sprayerin an amount of 216 L/ha. Then, the weeds were cultivated in agreenhouse for 17 days, followed by examining the herbicidal effects.For comparison, Amaranthus palmeri having Gly210 deletion in PPO2(originated from Illinois, USA) and commercially available wild-typeAmaranthus palmeri were tested. The results are shown in Tables 1 and 2.

TABLE 1 Amaranthus palmeri Application Gly399Ala Wild- Effect Compoundrate (g/ha) mutation type reduction Saflufenacil 25 100 100 0 point

TABLE 2 (Known art) Amaranthus palmeri Amount Gly210 Wild- EffectCompound (g/ha) deletion type reduction Saflufenacil 25 65 100 35 points

Example 4610

Plastic pots each having a diameter of 8 cm and a height of 7 cm werefilled with the soil and seedlings of Amaranthus palmeri havingArg128Gly mutation in PPO2 (originated from Arkansas, USA) were planted.After cultivaited in a greenhouse for 42 days, an aqueous spray liquidprepared by diluting a predetermined amount of trifludimoxazinformulation (emulsifiable concentrate obtained by mixing 20 parts oftrifludimoxazin with 80 parts liquid cocktail (5:1 mixture ofcyclohexanone and Sorpol 2680X (TOHO Chemical Industry Co., Ltd.))) withwater, and its mixture with saflufenacil made by additionally diluting apredetermined amount of commercial saflufenacil formulation (suspensionconcentrate containing 29.74% of saflufenacil, manufactured by BASFCorp. under the trade name of Sharpen) were separately sprayed over theweed using a sprayer in an amount of 200 L/ha uniformly. Then, the weedswere cultivated in a greenhouse for 4 days, followed by examininig theherbicidal effects on the weeds. For comparison, waterhemp having Gly210deletion in PPO2 (originated from Illinois, USA), commercially availablewild-type waterhemp, and commercially available wild-type Amaranthuspalmeri were tested. The results are shown in Tables 3 and 4.

TABLE 3 Amaranthus palmeri Application Arg128Gly Wild- Effect Compoundrate (g/ha) mutation type reduction Trifludimoxazin 100 88 90 2 pointsTrifludimoxazin + 50 + 85 88 3 points saflufenacil 100

TABLE 4 (Known art) waterhemp Application Gly210 Wild- Effect Compoundrate (g/ha) deletion type reduction Trifludimoxazin 100 50 95 45 pointsTrifludimoxazin + 50 + 35 95 60 points saflufenacil 100

INDUSTRIAL APPLICABILITY

Herbicide resistant weeds in a crop cultivation area can be efficientlycontrolled by the method of controlling herbicide resistant weedsaccording to the present invention.

1. A method of controlling herbicide resistant weeds, the methodcomprising: treating herbicide resistant weeds with one or more ofherbicides selected from the group consisting of saflufenacil,trifludimoxazin, flumioxazin, and ethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetate,wherein the herbicide resistant weeds are PPO-inhibitor resistant weedshaving one or more mutations selected from the group consisting of anArg128Met mutation, an Arg128Gly mutation, an Arg128His mutation, and aGly399Ala mutation in PPO.
 2. The control method of claim 1, wherein theherbicide resistant weed is Amaranthus palmeri, waterhemp, or ragweed.3. The control method of claim 1, wherein the method is performed incrop cultivation.
 4. The control method of claim 3, wherein the crop isa crop selected from the group consisting of a soybean, corn, cotton,rape seed, rice, wheat, barley, sugarcane, sugar beet, sorghum, andsunflower.
 5. The control method of claim 3, wherein the crop is a cropto which is tolerant to saflufenacil, trifludimoxazin, flumioxazin, orethyl[3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-3-yl)phenoxy]-2-pyridyloxy]acetate.6. The control method of claim 1, wherein the herbicide resistant weedis a lactofen resistant weed or a fomesafen resistant weed.